Construct: ORF TRCN0000489714
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020222.1_s317c1
- DNA Barcode:
- TTCAATTAGCACCCTCCCTTCAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DGKG (1608)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489714
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1608 | DGKG | diacylglycerol kinase gamma | NM_001080744.2 | 99.8% | 99.7% | 425C>G;477A>G;947G>A |
| 2 | human | 1608 | DGKG | diacylglycerol kinase gamma | NM_001346.3 | 96.7% | 96.5% | (many diffs) |
| 3 | human | 1608 | DGKG | diacylglycerol kinase gamma | NM_001080745.2 | 91.7% | 91.6% | (many diffs) |
| 4 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | NM_138650.2 | 84.7% | 86.7% | (many diffs) |
| 5 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | XM_017316850.1 | 80% | 81.9% | (many diffs) |
| 6 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | NM_001346673.1 | 79.9% | 81.7% | (many diffs) |
| 7 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | XM_017316851.1 | 75.5% | 77.2% | (many diffs) |
| 8 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | XM_017316852.1 | 70.5% | 71% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2370
- ORF length:
- 2298
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggtgaa gaacggtggg tctccctcac tccagaagaa tttgaccaac 121 tccagaaata ttcagaatat tcctccaaga agataaaaga tgccttgact gaatttaatg 181 agggtgggag cctcaaacaa tatgacccac atgagccgat tagctatgat gtcttcaagc 241 tgttcatgag ggcgtacctg gaggtggacc ttccccagcc actgagcact cacctcttcc 301 tggccttcag ccagaagccc agacacgaga cctctgacca cccgacggag ggagccagca 361 acagtgaggc caacagcgca gatactaata tacagaatgc agataatgcc accaaagcag 421 acgaggcctg tgcccctgat actgaatcaa atatggctga gaagcaagca ccagctgaag 481 accaagtggc tgcgagcccc ctggaacccc ccgtccctcg gtcttcaagc tcggaatccc 541 cagtggtgta cctgaaggat gttgtgtgct acctgtccct gctggagacg gggaggcctc 601 aggataagct ggagttcatg tttcgcctct atgattcaga tgagaacggt ctcctggacc 661 aagcggagat ggattgcatt gtcaaccaaa tgctgcatat tgcccagtac ctggagtggg 721 atcccacaga gctgaggcct atattgaagg agatgctgca agggatggac tacgaccggg 781 acggctttgt gtctctacag gaatgggtcc atggagggat gaccaccatc ccattgctgg 841 tcctcctggg gatggatgac tctggctcca agggggatgg gcggcacgcc tggaccatga 901 agcacttcaa gaaaccaacc tactgcaact tctgccatat catgctcatg ggcgtccgca 961 agcaaggcct gtgctgcact tactgtaaat acactgtcca cgaacgctgt gtgtccaaaa 1021 acattcctgg ttgtgtcaaa acgtactcaa aagccaaaag gagtggtgag gtgatgcagc 1081 acgcatgggt ggaagggaac tcctccgtca agtgtgaccg gtgccacaaa agtatcaagt 1141 gctaccagag tgtcaccgcg cggcactgcg tgtggtgccg gatgacgttt caccgcaaat 1201 gtgaattatc aacgttgtgt gacggtgggg aactcagaga ccacatctta ctgcccacct 1261 ccatatgccc catcacccgg gacaggccag gtgagaagtc tgatggctgc gtgtccgcca 1321 agggcgaact tgtcatgcag tataagatca tccccacccc gggtacccac cccctgctgg 1381 tcttggtgaa ccccaagagt ggagggagac aaggagaaag gttgaacttt ttccgtgata 1441 ctccagactt ccgtgttttg gcctgtggtg gagatgggac agttggctgg attttggatt 1501 gcattgataa ggccaacttt gcaaagcatc caccagtggc tgtcctgcct cttggaacag 1561 gaaatgacct tgcccgttgt ctccgctggg gaggaggtta tgaagggggc agcttgacaa 1621 aaatcctgaa agacattgag cagagcccct tggtgatgct ggaccgctgg catctggaag 1681 tcatccccag agaggaagtg gaaaacgggg accaggtccc atacagcatc atgaacaact 1741 atttctccat tggtgtggac gcttccattg cacacagatt ccatgtgatg agagagaaac 1801 atcctgaaaa attcaacagc aggatgaaga acaagctgtg gtactttgaa tttggcacct 1861 cggagacttt tgcagcgacc tgcaagaaac tccacgacca cattgagttg gagtgtgatg 1921 gggttggggt ggacctgagc aacaTCTTCC TGGAAGGCAT TGCCATTCTC AACATTCCCA 1981 GCATGTACGG AGGCACCAAT CTCTGGGGAG AAAACAAGAA GAACCGGGCT GTGATCCGGG 2041 AAAGCAGGAA GGGTGTCACT GACCCCAAAG AACTGAAATT CTGCGTTCAA GACCTCAGTG 2101 ACCAGCTCCT TGAAGTGGTG GGGCTAGAAG GAGCCATGGA GATGGGGCAG ATCTACACCG 2161 GCCTGAAGAG TGCAGGCAGG AGGCTGGCCC AGTGCGCCTC TGTCACCATC AGGACAAACA 2221 AGCTGCTGCC AATGCAAGTG GATGGAGAAC CCTGGATGCA GCCATGTTGC ACGATTAAAA 2281 TTACTCACAA GAACCAAGCG CCCATGATGA TGGGGCCTCC CCAGAAGAGC AGCTTCTTCT 2341 CGTTGAGAAG GAAGAGCCGT TCAAAAGACT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG 2401 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2461 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 2521 CAATTAGCAC CCTCCCTTCA GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 2581 ttgtgaaaga tt