Construct: ORF TRCN0000489726
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021800.1_s317c1
- DNA Barcode:
- ATTTAATATAAGCCTCAACCCCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC5A11 (115584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489726
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001352242.2 | 100% | 100% | |
| 2 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001352248.2 | 100% | 100% | |
| 3 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_052944.4 | 100% | 100% | |
| 4 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | XM_017022903.1 | 100% | 100% | |
| 5 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001352250.2 | 96% | 96% | 583_584ins81 |
| 6 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001352235.2 | 95.5% | 94.2% | (many diffs) |
| 7 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001258411.2 | 94.8% | 94.8% | 371_372ins105 |
| 8 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001352238.2 | 94.8% | 94.8% | 371_372ins105 |
| 9 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001258413.3 | 90.5% | 90.5% | 0_1ins192 |
| 10 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001352236.2 | 90.5% | 90.5% | 0_1ins192 |
| 11 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001352249.2 | 90.5% | 90.5% | 0_1ins192 |
| 12 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001352259.2 | 90.5% | 90.5% | 0_1ins192 |
| 13 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | XM_011545725.2 | 90.3% | 89.1% | (many diffs) |
| 14 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001258412.2 | 89.6% | 89.6% | 207_208ins105;266_267ins105 |
| 15 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001352245.2 | 86.3% | 77.6% | 664_665ins206;904_905ins70 |
| 16 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001352246.2 | 86.3% | 77.6% | 664_665ins206;904_905ins70 |
| 17 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001352237.2 | 85.3% | 85.3% | 0_1ins192;179_180ins105 |
| 18 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NR_147936.2 | 85.2% | (many diffs) | |
| 19 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NR_147940.2 | 81.6% | (many diffs) | |
| 20 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NR_147934.2 | 81.4% | 1_281del;1391_1392ins70;2237_2329del | |
| 21 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001352247.2 | 81.1% | 72.4% | 371_372ins105;559_560ins206;799_800ins70 |
| 22 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NR_147935.2 | 79.1% | 1_353del;1463_1464ins70;2309_2401del | |
| 23 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NR_147933.2 | 77.1% | (many diffs) | |
| 24 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NM_001258414.1 | 76.8% | 68.1% | 0_1ins192;472_473ins206;712_713ins70 |
| 25 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | XM_005255082.4 | 76.1% | 76.1% | 0_1ins483 |
| 26 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | XM_024450145.1 | 76.1% | 76.1% | 0_1ins483 |
| 27 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NR_147938.2 | 74.7% | (many diffs) | |
| 28 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | NR_147939.2 | 74.2% | (many diffs) | |
| 29 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | XM_024450146.1 | 62.5% | 53.7% | 0_1ins483;181_182ins206;421_422ins70 |
| 30 | human | 115584 | SLC5A11 | solute carrier family 5 mem... | XM_011545728.1 | 54% | 54% | 0_1ins930 |
| 31 | mouse | 233836 | Slc5a11 | solute carrier family 5 (so... | NM_146198.2 | 85.2% | 86.3% | (many diffs) |
| 32 | mouse | 233836 | Slc5a11 | solute carrier family 5 (so... | XM_006507712.3 | 85.2% | 86.3% | (many diffs) |
| 33 | mouse | 233836 | Slc5a11 | solute carrier family 5 (so... | XM_011241766.2 | 85.2% | 86.3% | (many diffs) |
| 34 | mouse | 233836 | Slc5a11 | solute carrier family 5 (so... | XM_011241767.2 | 85.2% | 86.3% | (many diffs) |
| 35 | mouse | 233836 | Slc5a11 | solute carrier family 5 (so... | XM_011241768.2 | 85.2% | 86.3% | (many diffs) |
| 36 | mouse | 233836 | Slc5a11 | solute carrier family 5 (so... | XM_011241769.1 | 71.6% | 72.5% | (many diffs) |
| 37 | mouse | 233836 | Slc5a11 | solute carrier family 5 (so... | XR_001785540.1 | 63.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2094
- ORF length:
- 2025
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggagagcggc accagcagcc ctcagcctcc acagttagat cccctggatg 121 cgtttcccca gaagggcttg gagcctgggg acatcgcggt gctagttctg tacttcctct 181 ttgtcctggc tgttggacta tggtccacag tgaagaccaa aagagacaca gtgaaaggct 241 acttcctggc tggaggggac atggtgtggt ggccagtggg tgcatccttg tttgccagca 301 atgttggaag tggacatttc attggcctgg cagggtcagg tgctgctacg ggcatttctg 361 tatcagctta tgaacttaat ggcttgtttt ctgtgctgat gttggcctgg atcttcctac 421 ccatctacat tgctggtcag gtcaccacga tgccagaata cctacggaag cgcttcggtg 481 gcatcagaat ccccatcatc ctggctgtac tctacctatt tatctacatc ttcaccaaga 541 tctcggtaga catgtatgca ggtgccatct tcatccagca gtctttgcac ctggatctgt 601 acctggccat agttgggcta ctggccatca ctgctgtata cacggttgct ggtggcctgg 661 ctgctgtgat ctacacggat gccctgcaga cgctgatcat gcttatagga gcgctcacct 721 tgatgggcta cagtttcgcc gcggttggtg ggatggaagg actgaaggag aagtacttct 781 tggccctggc tagcaaccgg agtgagaaca gcagctgcgg gctgccccgg gaagatgcct 841 tccatatttt ccgagatccg ctgacatctg atctcccgtg gccgggggtc ctatttggaa 901 tgtccatccc atccctctgg tactggtgca cggatcaggt gattgtccag cggactctgg 961 ctgccaagaa cctgtcccat gccaaaggag gtgctctgat ggctgcatac ctgaaggtgc 1021 tgcccctctt cataatggtg ttccctggga tggtcagccg catcctcttc ccagatcaag 1081 tggcctgtgc agatccagag atctgccaga agatctgcag caacccctca ggctgttcgg 1141 acatcgcgta tcccaaactc gtgctggaac tcctgcccac agggctccgt gggctgatga 1201 tggctgtgat ggtggcggct ctcatgtcct ccctcacctc catctttaac agtgccagca 1261 ccatcttcac catggacctc tggaatcacc tccggcctcg ggcatctgag aaggagctca 1321 tgattgtggg cagggtgttt gtgctgctgc tggtcctggt ctccatcctc tggatccctg 1381 tggtccaggc cagccagggc ggccagctct tcatctatat ccagtccatc agctcctacc 1441 tgcagccgcc tgtggcggtg gtcttcatca tgggatgttt ctggaagagg accaatgaaa 1501 agggtgcctt ctggggcctg atctcgggcc tgctcctggg cttggttagg ctggtcctgg 1561 actttattta cgtgcagcct cgatgcgacc agccagatga gcgcccggtc ctggtgaaga 1621 gcattcacta cctctacttc tccatgatcc tgtccacggt caccctcatc actgtctcca 1681 ccgtgagctg gttcacagag ccaccctcca aggagatggT CAGCCACCTG ACCTGGTTTA 1741 CTCGTCACGA CCCCGTGGTC CAGAAGGAAC AAGCACCACC AGCAGCTCCC TTGTCTCTTA 1801 CCCTCTCTCA GAACGGGATG CCAGAGGCCA GCAGCAGCAG CAGCGTCCAG TTCGAGATGG 1861 TTCAAGAAAA CACGTCTAAA ACCCACAGCT GTGACATGAC CCCAAAGCAG TCCAAAGTGG 1921 TGAAGGCCAT CCTGTGGCTC TGTGGAATAC AGGAGAAGGG CAAGGAAGAG CTCCCGGCCA 1981 GAGCAGAAGC CATCATAGTT TCCCTGGAAG AAAACCCCTT GGTGAAGACC CTCCTGGACG 2041 TCAACCTCAT TTTCTGCGTG AGCTGCGCCA TCTTTATCTG GGGCTATTTT GCTTAGAACC 2101 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2161 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2221 ATATATCTTG TGGAAAGGAC GAATTTAATA TAAGCCTCAA CCCCCGACGC GTTAAGTCga 2281 caatcaacct ctggattaca aaatttgtga aagatt