Construct: ORF TRCN0000489758
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020544.1_s317c1
- DNA Barcode:
- TCAGGGTCTGCACCATACTCATCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAPK6 (5597)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489758
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5597 | MAPK6 | mitogen-activated protein k... | NM_002748.4 | 99.9% | 99.8% | 868C>G |
| 2 | human | 5597 | MAPK6 | mitogen-activated protein k... | XM_005254537.3 | 99.9% | 99.8% | 868C>G |
| 3 | human | 5597 | MAPK6 | mitogen-activated protein k... | XM_005254538.2 | 99.9% | 99.8% | 868C>G |
| 4 | human | 5597 | MAPK6 | mitogen-activated protein k... | XM_011521782.1 | 99.9% | 99.8% | 868C>G |
| 5 | mouse | 50772 | Mapk6 | mitogen-activated protein k... | NM_015806.5 | 87.7% | 94.4% | (many diffs) |
| 6 | mouse | 50772 | Mapk6 | mitogen-activated protein k... | NM_027418.2 | 87.7% | 94.4% | (many diffs) |
| 7 | mouse | 50772 | Mapk6 | mitogen-activated protein k... | XM_011242767.2 | 87.7% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2235
- ORF length:
- 2163
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcagag aaatttgaaa gtctcatgaa cattcatggt tttgatctgg 121 gttctaggta tatggactta aaaccattgg gttgtggagg caatggcttg gttttttctg 181 ctgtagacaa tgactgtgac aaaagagtag ccatcaagaa aattgtcctt actgatcccc 241 agagtgtcaa acatgctcta cgtgaaatca aaattattag aagacttgac catgataaca 301 ttgtgaaagt gtttgagatt cttggtccca gtggaagcca attaacagac gatgtgggct 361 ctcttacgga actgaacagt gtttacattg ttcaggagta catggagaca gacttggcta 421 atgtgctgga gcagggccct ttactggaag agcatgccag gcttttcatg tatcagctgc 481 tacgggggct caagtatatt cactctgcaa atgtactgca cagagatctc aaaccagcta 541 atcttttcat taatacggaa gacttggtgc tgaagatagg tgactttggt cttgcacgga 601 tcatggatcc tcattattcc cataagggtc atctttctga aggattggtt actaaatggt 661 acagatctcc acgtctttta ctttctccta ataattatac taaagccatt gacatgtggg 721 ctgcaggctg catctttgct gaaatgctga ctggtaaaac cctttttgca ggtgcacatg 781 aacttgaaca gatgcagctg attttagaat ctattcctgt tgtacatgag gaagatcgtc 841 aggagcttct cagcgtaatt ccagtttaca ttagaaatga catgactgag ccacacaaac 901 ctttaactca gctgcttcca ggaattagtc gagaagcagt ggatttcctg gaacaaattt 961 tgacatttag ccccatggat cggttaacag cagaagaagc actctcccat ccttacatga 1021 gcatatattc ttttccaatg gatgagccaa tttcaagcca tccttttcat attgaagatg 1081 aagttgatga tattttgctt atggatgaaa ctcacagtca catttataac tgggaaaggt 1141 atcatgattg tcagttttca gagcatgatt ggcctgtaca taacaacttt gatattgatg 1201 aagttcagct tgatccaaga gctctgtccg atgtcactga tgaagaagaa gtacaagttg 1261 atccccgaaa atatttggat ggagatcggg aaaagtatct ggaggatcct gcttttgata 1321 ccaattactc tactgagcct tgttggcaat actcagatca tcatgaaaac aaatattgtg 1381 atctggagtg tagccatact tgtaactaca aaacgaggtc atcatcatat ttagataact 1441 tagtttggag agagagtgaa gttaaccatt actatgaacc caagcttatt atagatcttt 1501 ccaattggaa agaacaaagc aaagaaaaat ctgataagaa aggcaaatca aaatgtgaaa 1561 ggaatggatt ggttaaagcc cagatagcgc tagaggaagc atcacagcaa ctggctggaa 1621 aagaaaggga aaagaatcag ggatttgatt ttgattcctt tattgcagga actattcagc 1681 ttagttccca gcatgagcct actgatgttg ttgataaatt aaatgacttg aatagctcag 1741 tgtcccaact agaattgaaa agtttgatat caaagtcagt aagccaagaa aaacaggaaa 1801 aaggaatggc aaaTCTGGCT CAATTAGAAG CCTTGTACCA GTCTTCTTGG GACAGCCAGT 1861 TTGTGAGTGG TGGGGAGGAC TGTTTTTTCA TAAATCAGTT TTGTGAGGTA AGGAAGGATG 1921 AACAAGTTGA GAAGGAAAAC ACTTACACTA GTTACTTGGA CAAGTTCTTT AGCAGGAAAG 1981 AAGATACTGA AATGCTAGAA ACTGAGCCAG TAGAGGATGG GAAGCTTGGG GAGAGAGGAC 2041 ATGAGGAAGG ATTTCTGAAC AACAGTGGGG AGTTCCTCTT TAACAAGCAG CTCGAGTCCA 2101 TAGGCATCCC ACAGTTTCAC AGTCCAGTTG GGTCACCACT TAAGTCAATA CAGGCCACAT 2161 TAACACCTTC TGCTATGAAA TCTTCCCCTC AAATTCCTCA TCAAACATAC AGCAGCATTC 2221 TGAAACATCT GAACTAGGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2281 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2341 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCAGGGT CTGCACCATA 2401 CTCATCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt