Construct: ORF TRCN0000489765
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019777.1_s317c1
- DNA Barcode:
- AGATTGATGGAGATGCTAGTGCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRK3 (157)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489765
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 157 | GRK3 | G protein-coupled receptor ... | NM_005160.4 | 99.9% | 99.8% | 2049A>G;2064_2065insG |
| 2 | human | 157 | GRK3 | G protein-coupled receptor ... | NM_001362778.2 | 83.4% | 83.4% | 0_1ins339;1710A>G;1725_1726insG |
| 3 | human | 157 | GRK3 | G protein-coupled receptor ... | XM_011529975.2 | 75.9% | 75.9% | 0_1ins495;1554A>G;1569_1570insG |
| 4 | human | 157 | GRK3 | G protein-coupled receptor ... | XR_937823.1 | 22.7% | (many diffs) | |
| 5 | human | 157 | GRK3 | G protein-coupled receptor ... | XR_001755175.1 | 22.6% | (many diffs) | |
| 6 | mouse | 320129 | Grk3 | G protein-coupled receptor ... | NM_177078.4 | 83% | 91.2% | (many diffs) |
| 7 | mouse | 320129 | Grk3 | G protein-coupled receptor ... | NM_001285806.1 | 69.6% | 76.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2139
- ORF length:
- 2067
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcggac ctggaggctg tgctggccga tgtcagttac ctgatggcca 121 tggagaagag caaggcgacc ccggccgccc gcgccagcaa gaggatcgtc ctgccggagc 181 ccagtatccg gagtgtgatg cagaagtacc ttgcagagag aaatgaaata acctttgaca 241 agattttcaa tcagaaaatt ggtttcttgc tatttaaaga tttttgtttg aatgaaatta 301 atgaagctgt acctcaggtg aagttttatg aagagataaa ggaatatgaa aaacttgata 361 atgaggaaga ccgcctttgc agaagtcgac aaatttatga tgcctacatc atgaaggaac 421 ttctttcctg ttcacatcct ttctcaaagc aagctgtaga acacgtacaa agtcatttat 481 ccaagaaaca agtgacatca actctttttc agccatacat agaagaaatt tgtgaaagcc 541 ttcgaggtga catttttcaa aaatttatgg aaagtgacaa gttcactaga ttttgtcagt 601 ggaaaaacgt tgaattaaat atccatttga ccatgaatga gttcagtgtg cataggatta 661 ttggacgagg aggattcggg gaagtttatg gttgcaggaa agcagacact ggaaaaatgt 721 atgcaatgaa atgcttagat aagaagagga tcaaaatgaa acaaggagaa acattagcct 781 taaatgaaag aatcatgttg tctcttgtca gcacaggaga ctgtcctttc attgtatgta 841 tgacctatgc cttccatacc ccagataaac tctgcttcat cctggatctg atgaacgggg 901 gcgatttgca ctaccacctt tcacaacacg gtgtgttctc tgagaaggag atgcggtttt 961 atgccactga aatcattctg ggtctggaac acatgcacaa tcggtttgtt gtctacagag 1021 atttgaagcc agcaaatatt ctcttggatg aacatggaca cgcaagaata tcagatcttg 1081 gtcttgcctg cgatttttcc aaaaagaagc ctcatgcgag tgttggcacc catgggtaca 1141 tggctcccga ggtgctgcag aaggggacgg cctatgacag cagtgccgac tggttctccc 1201 tgggctgcat gcttttcaaa cttctgagag gtcacagccc tttcagacaa cataaaacca 1261 aagacaagca tgaaattgac cgaatgacac tcaccgtgaa tgtggaactt ccagacacct 1321 tctctcctga actgaagtcc cttttggagg gcttgcttca gcgagacgtt agcaagcggc 1381 tgggctgtca cggaggcggc tcacaggaag taaaagagca cagctttttc aaaggtgttg 1441 actggcagca tgtctactta caaaagtacc caccaccctt gattcctccc cggggagaag 1501 tcaatgctgc tgatgccttt gatattggct catttgatga agaggatacc aaagggatta 1561 agctacttga ttgcgaccaa gaactctaca agaacttccc tttggtcatc tctgaacgct 1621 ggcagcaaga agtaacggaa acagtttatg aagcagtaaa tgcagacaca gataaaatcg 1681 aggccaggaa gagagctaaa aataagcaac ttggccacga agaagattac gctctgggga 1741 aggactgtat tatgcacggg tacatgctga aactgggaaa cccatttctg actcagtggc 1801 agcgtcgcta tttttacctc tttccaaata gacttgaatg gagaggagag ggagagtccc 1861 ggcaaaattt actgacaatg gaacagaTTC TCTCTGTGGA AGAAACTCAA ATTAAAGACA 1921 AAAAATGCAT TTTGTTCAGA ATAAAAGGAG GGAAACAATT TGTCTTGCAA TGTGAGAGTG 1981 ATCCAGAGTT TGTGCAGTGG AAGAAAGAGT TGAACGAAAC CTTCAAGGAG GCCCAGCGGC 2041 TATTGCGTCG TGCCCCGAAG TTCCTCAACA AACCTCGGTC AGGTACTGTG GAGCTCCCAA 2101 AGCCATCCCT CTGTCACAGG AACAGCAACG GCCTCGACCC AGCTTTCTTG TACAAAGTGG 2161 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2221 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2281 AAGATTGATG GAGATGCTAG TGCATACGCG TTAAGTCgac aatcaacctc tggattacaa 2341 aatttgtgaa agatt