Construct: ORF TRCN0000489778
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020577.1_s317c1
- DNA Barcode:
- TGGGGATCTGCTTCATCGTGAGCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PAK6 (56924)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489778
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 106821730 | BUB1B-PAK6 | BUB1B-PAK6 readthrough | NM_001128628.2 | 99.9% | 100% | 249G>A |
| 2 | human | 106821730 | BUB1B-PAK6 | BUB1B-PAK6 readthrough | NM_001128629.3 | 99.9% | 100% | 249G>A |
| 3 | human | 56924 | PAK6 | p21 (RAC1) activated kinase 6 | NM_001276717.1 | 99.9% | 100% | 249G>A |
| 4 | human | 56924 | PAK6 | p21 (RAC1) activated kinase 6 | NM_020168.5 | 99.9% | 100% | 249G>A |
| 5 | human | 56924 | PAK6 | p21 (RAC1) activated kinase 6 | NM_001276718.2 | 93.3% | 93.3% | 249G>A;1741_1742ins135 |
| 6 | mouse | 214230 | Pak6 | p21 protein (Cdc42/Rac)-act... | NM_001033254.3 | 88.7% | 92.5% | (many diffs) |
| 7 | mouse | 214230 | Pak6 | p21 protein (Cdc42/Rac)-act... | NM_001145854.1 | 88.7% | 92.5% | (many diffs) |
| 8 | mouse | 214230 | Pak6 | p21 protein (Cdc42/Rac)-act... | XM_011239419.1 | 88.7% | 92.5% | (many diffs) |
| 9 | mouse | 214230 | Pak6 | p21 protein (Cdc42/Rac)-act... | XM_011239420.2 | 88.7% | 92.5% | (many diffs) |
| 10 | mouse | 214230 | Pak6 | p21 protein (Cdc42/Rac)-act... | XR_866265.2 | 51.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2115
- ORF length:
- 2043
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgttccgc aagaaaaaga agaaacgccc tgagatctca gcgccacaga 121 acttccagca ccgtgtccac acctccttcg accccaaaga aggcaagttt gtgggcctcc 181 ccccacaatg gcagaacatc ctggacacac tgcggcgccc caagcccgtg gtggaccctt 241 cgcgaatcac acgggtgcag ctccagccca tgaagacagt ggtgcggggc agcgcgatgc 301 ctgtggatgg ctacatctca gggctgctca acgacatcca gaagttgtca gtcatcagct 361 ccaacaccct gcgtggccgc agccccacca gccggcggcg ggcacagtcc ctggggctgc 421 tgggggatga gcactgggcc accgacccag acatgtacct ccagagcccc cagtctgagc 481 gcactgaccc ccacggcctc tacctcagct gcaacggggg cacaccagca ggccacaagc 541 agatgccgtg gcccgagcca cagagcccac gggtcctgcc caatgggctg gctgcaaagg 601 cacagtccct gggccccgcc gagtttcagg gtgcctcgca gcgctgtctg cagctgggtg 661 cctgcctgca gagctcccca ccaggagcct cgccccccac gggcaccaat aggcatggaa 721 tgaaggctgc caagcatggc tctgaggagg cccggccaca gtcctgcctg gtgggctcag 781 ccacaggcag gccaggtggg gaaggcagcc ctagccctaa gacccgggag agcagcctga 841 agcgcaggct attccgaagc atgttcctgt ccactgctgc cacagcccct ccaagcagca 901 gcaagccagg ccctccacca cagagcaagc ccaactcctc tttccgaccg ccgcagaaag 961 acaacccccc aagcctggtg gccaaggccc agtccttgcc ctcggaccag ccggtgggga 1021 ccttcagccc tctgaccact tcggatacca gcagccccca gaagtccctc cgcacagccc 1081 cggccacagg ccagcttcca ggccggtctt ccccagcggg atccccccgc acctggcacg 1141 cccagatcag caccagcaac ctgtacctgc cccaggaccc cacggttgcc aagggtgccc 1201 tggctggtga ggacacaggt gttgtgacac atgagcagtt caaggctgcg ctcaggatgg 1261 tggtggacca gggtgacccc cggctgctgc tggacagcta cgtgaagatt ggcgagggct 1321 ccaccggcat cgtctgcttg gcccgggaga agcactcggg ccgccaggtg gccgtcaaga 1381 tgatggacct caggaagcag cagcgcaggg agctgctctt caacgaggtg gtgatcatgc 1441 gggactacca gcacttcaac gtggtggaga tgtacaagag ctacctggtg ggcgaggagc 1501 tgtgggtgct catggagttc ctgcagggag gagccctcac agacatcgtc tcccaagtca 1561 ggctgaatga ggagcagatt gccactgtgt gtgaggctgt gctgcaggcc ctggcctacc 1621 tgcatgctca gggtgtcatc caccgggaca tcaagagtga ctccatcctg ctgaccctcg 1681 atggcagggt gaagctctcg gacttcggat tctgtgctca gatcagcaaa gacgtcccta 1741 agaggaagtc cctggtggga accccctACT GGATGGCTCC TGAAGTGATC TCCAGGTCTT 1801 TGTATGCCAC TGAGGTGGAT ATCTGGTCTC TGGGCATCAT GGTGATTGAG ATGGTAGATG 1861 GGGAGCCACC GTACTTCAGT GACTCCCCAG TGCAAGCCAT GAAGAGGCTC CGGGACAGCC 1921 CCCCACCCAA GCTGAAAAAC TCTCACAAGG TCTCCCCAGT GCTGCGAGAC TTCCTGGAGC 1981 GGATGCTGGT GCGGGACCCC CAAGAGAGAG CCACAGCCCA GGAGCTCCTA GACCACCCCT 2041 TCCTGCTGCA GACAGGGCTA CCTGAGTGCC TGGTGCCCCT GATCCAGCTC TACCGAAAGC 2101 AGACCTCCAC CTGCTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2161 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2221 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGGGGAT CTGCTTCATC 2281 GTGAGCGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt