Construct: ORF TRCN0000489784
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019207.1_s317c1
- DNA Barcode:
- AAGCATTTGTCTGCCTTACTAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GHSR (2693)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489784
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2693 | GHSR | growth hormone secretagogue... | NM_198407.2 | 99.8% | 100% | 171C>T;477G>A |
| 2 | human | 2693 | GHSR | growth hormone secretagogue... | NM_004122.2 | 77% | 71.7% | (many diffs) |
| 3 | mouse | 208188 | Ghsr | growth hormone secretagogue... | NM_177330.4 | 89.5% | 95.3% | (many diffs) |
| 4 | mouse | 208188 | Ghsr | growth hormone secretagogue... | XM_017319513.1 | 76.3% | 81.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1173
- ORF length:
- 1098
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgtgg aacgcgacgc ccagcgaaga gccggggttc aacctcacac 121 tggccgacct ggactgggat gcttcccccg gcaacgactc gctgggcgac gagctgctgc 181 agctcttccc cgcgccgctg ctggcgggcg tcacagccac ctgcgtggca ctcttcgtgg 241 tgggtatcgc tggcaacctg ctcaccatgc tggtggtgtc gcgcttccgc gagctgcgca 301 ccaccaccaa cctctacctg tccagcatgg ccttctccga tctgctcatc ttcctctgca 361 tgcccctgga cctcgttcgc ctctggcagt accggccctg gaacttcggc gacctcctct 421 gcaaactctt ccaattcgtc agtgagagct gcacctacgc cacggtgctc accatcacag 481 cgctgagcgt cgagcgctac ttcgccatct gcttcccact ccgggccaag gtggtggtca 541 ccaaggggcg agtgaagctg gtcatcttcg tcatctgggc cgtggccttc tgcagcgccg 601 ggcccatctt cgtgctagtc ggggtggagc acgagaacgg caccgaccct tgggacacca 661 acgagtgccg ccccaccgag tttgcggtgc gctctggact gctcacggtc atggtgtggg 721 tgtccagcat cttcttcttc cttcctgtct tctgtctcac ggtcctctac agTCTCATCG 781 GCAGGAAGCT GTGGCGGAGG AGGCGCGGCG ATGCTGTCGT GGGTGCCTCG CTCAGGGACC 841 AGAACCACAA GCAAACCGTG AAAATGCTGG CTGTAGTGGT GTTTGCCTTC ATCCTCTGCT 901 GGCTCCCCTT CCACGTAGGG CGATATTTAT TTTCCAAATC CTTTGAGCCT GGCTCCTTGG 961 AGATTGCTCA GATCAGCCAG TACTGCAACC TCGTGTCCTT TGTCCTCTTC TACCTCAGTG 1021 CTGCCATCAA CCCCATTCTG TACAACATCA TGTCCAAGAA GTACCGGGTG GCAGTGTTCA 1081 GACTTCTGGG ATTCGAACCC TTCTCCCAGA GAAAGCTCTC CACTCTGAAA GATGAAAGTT 1141 CTCGGGCCTG GACAGAATCT AGTATTAATA CAGACCCAGC TTTCTTGTAC AAAGTGGTTG 1201 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1261 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 1321 GCATTTGTCT GCCTTACTAA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1381 ttgtgaaaga tt