Construct: ORF TRCN0000489794
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019423.1_s317c1
- DNA Barcode:
- TGTAGGTGAACCTTGAATTACATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K13 (9175)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489794
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9175 | MAP3K13 | mitogen-activated protein k... | NM_001242314.2 | 99.9% | 99.8% | 2898_2899insG |
| 2 | human | 9175 | MAP3K13 | mitogen-activated protein k... | NM_004721.5 | 99.9% | 99.8% | 2898_2899insG |
| 3 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_011513310.2 | 99.9% | 99.8% | 2898_2899insG |
| 4 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_017007456.1 | 99.9% | 99.8% | 2898_2899insG |
| 5 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_017007457.1 | 84.3% | 83.3% | (many diffs) |
| 6 | human | 9175 | MAP3K13 | mitogen-activated protein k... | NM_001242317.2 | 77.7% | 77.1% | (many diffs) |
| 7 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_017007458.1 | 75.9% | 75.9% | 0_1ins696;2202_2203insG |
| 8 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_017007459.2 | 75.9% | 75.9% | 0_1ins696;2202_2203insG |
| 9 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | NM_172821.3 | 84.5% | 88.9% | (many diffs) |
| 10 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_006522582.3 | 84.5% | 88.9% | (many diffs) |
| 11 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_006522583.3 | 84.5% | 88.9% | (many diffs) |
| 12 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_006522584.3 | 84.5% | 88.9% | (many diffs) |
| 13 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_011246015.2 | 84.5% | 88.9% | (many diffs) |
| 14 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_011246016.2 | 84.5% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2967
- ORF length:
- 2901
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat 61 tgaccatggc caactttcag gagcacctga gctgctcctc ttctccacac ttacccttca 121 gtgaaagcaa aaccttcaat ggactacaag atgagctcac agctatgggg aaccaccctt 181 ctcccaagct gctcgaggac cagcaggaaa aggggatggt acgaacagag ctaatcgaga 241 gcgtgcacag ccccgtcacc acaacagtgt tgacgagcgt aagtgaggat tccagggacc 301 agtttgagaa cagcgttctt cagctaaggg aacacgatga atcagagacg gcggtgtctc 361 aggggaacag caacacggtg gacggagaga gcacaagcgg aactgaagac ataaagattc 421 agttcagcag gtcaggcagt ggcagtggtg ggtttcttga aggactattt ggatgcttaa 481 ggcctgtatg gaatatcatt gggaaggcat attccactga ttacaaattg cagcagcaag 541 atacttggga agtgccattt gaggagatct cagagctgca gtggctgggt agtggagccc 601 aaggagcggt cttcttgggc aagttccggg cggaagaggt ggccatcaag aaagtgagag 661 aacagaatga gacggatatc aagcatttga ggaagttgaa gcaccctaac atcatcgcat 721 tcaagggtgt ttgtactcag gccccatgtt attgtattat catggaatac tgtgcccatg 781 gacaactcta cgaggtctta cgagctggca ggaagatcac acctcgattg ctagtagact 841 ggtccacagg aattgcaagt ggaatgaatt atttgcacct ccataaaatt attcatcgtg 901 atctcaaatc acctaatgtt ttagtgaccc acacagatgc ggtaaaaatt tcagattttg 961 gtacatctaa ggaactcagt gacaaaagta ccaagatgtc atttgctggc acggtcgcat 1021 ggatggcgcc agaggtgata cggaatgaac ctgtctctga aaaagttgat atatggtctt 1081 ttggagtggt gctttgggag ctgctgacag gagagatccc ttacaaagat gtagattctt 1141 cagccattat ctggggtgtt ggaagcaaca gcctccacct tccagttcct tccacttgcc 1201 ctgatggatt caaaatcctt atgaaacaga cgtggcagag taaacctcga aaccgacctt 1261 cttttcggca gacactcatg catttagaca ttgcctctgc agatgtactt gccaccccac 1321 aagaaactta cttcaagtct caggctgaat ggagagaaga agtgaaaaaa cattttgaga 1381 agatcaaaag tgaaggaact tgtatacacc ggttagatga agaactgatt cgaaggcgca 1441 gagaagagct caggcatgcg ctggatattc gtgaacacta tgagcggaag cttgagcggg 1501 cgaataattt atacatggaa ttgagtgcca tcatgctgca gctagaaatg cgggagaagg 1561 agctcattaa gcgtgagcaa gcagtggaaa agaagtatcc tgggacctac aaacgacacc 1621 ctgttcgtcc tatcatccat cccaatgcca tggagaaact catgaaaagg aaaggagtgc 1681 ctcacaaatc tgggatgcag accaaacggc cagacttgtt gagatcagaa gggatcccca 1741 ccacagaagt ggctcccact gcatcccctt tgtccggaag tcccaaaatg tccacttcta 1801 gcagcaagag ccgatatcga agcaaaccac gccaccgccg agggaatagc agaggcagcc 1861 atagtgactt tgccgcaatc ttgaaaaacc agccagccca ggaaaattca ccccatccca 1921 cttacctgca ccaagctcaa tcccaatacc cttctcttca tcaccataat tctctgcagc 1981 agcaatacca gcagccccct cctgccatgt cccagagtca ccatcccaga ctcaatatgc 2041 acggacagga catagcaacc tgcgccaaca acctgaggta tttcggccca gcagcagccc 2101 tgcggagccc actcagcaac catgctcaga gacagctgcc cggctcgagc cctgacctca 2161 tctccacagc catggctgca gactgctgga gaagttctga gcctgacaag ggccaagctg 2221 gtccctgggg ctgttgccag gctgacgctt atgacccctg ccttcagtgc aggccagaac 2281 agtatgggtc cttagacata ccctctgctg agccagtggg gaggagccct gacctttcca 2341 agtcaccagc acataatcct ctcttggaaa acgcccagag ttctgagaaa acggaagaaa 2401 atgaattcag cggctgtagg tctgagtcat ccctcggcac ctctcatctc ggcacccctc 2461 cagcgctacc tcgaaaaaca aggcctctgc agaagagtgg agatgactcc tcagaagagg 2521 aagaagggga agtagatagt gaagttgaat ttccacgaag acagaggccc catcgctgta 2581 tcagcagctg ccagtcatat tcaaccttta gctctgagaa tttctctgtg tctgatggag 2641 aagagggaaa taccagtgac cactcaaaca gtcctgatga gttagctgat aaacttgaag 2701 accgcttggc agagaagcta gacgacctgc tgtcccagac gccagagatt cccattgaca 2761 tatcctcaca ctcggatggg CTCTCTGACA AGGAGTGTGC CGTGCGCCGT GTGAAGACTC 2821 AGATGTCTCT GGGCAAGCTG TGTGTGGAGG AACGTGGCTA TGAGAACCCC ATGCAGTTTG 2881 AAGAATCGGA CTGTGACTCT TCAGATGGGG AGTGTTCTGA TGCCACAGTT AGGACCAATA 2941 AACACTACAG CTCTGCTACC TGGGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 3001 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 3061 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GTAGGTGAAC 3121 CTTGAATTAC ATGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 3181 att