Construct: ORF TRCN0000489814
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019552.1_s317c1
- DNA Barcode:
- GCGCCGAGATCCTTTGTAATTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADGRE5 (976)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489814
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 976 | ADGRE5 | adhesion G protein-coupled ... | NM_001784.5 | 99.9% | 100% | 2225_2226delTA |
| 2 | human | 976 | ADGRE5 | adhesion G protein-coupled ... | NM_001025160.2 | 94.3% | 94.4% | 345_476del;2357_2358delTA |
| 3 | human | 976 | ADGRE5 | adhesion G protein-coupled ... | NM_078481.4 | 88.7% | 88.8% | 344_622del;2504_2505delTA |
| 4 | human | 976 | ADGRE5 | adhesion G protein-coupled ... | XM_011528451.3 | 87.8% | 87.7% | 344_622del;2479_2480delAAinsGC;2481_2482ins22 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 2301
- ORF length:
- 2226
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggga ggccgcgtct ttctcgcatt ctgtgtctgg ctgactctgc 121 cgggagctga aacccaggac tccaggggct gtgcccggtg gtgccctcag aactcctcgt 181 gtgtcaatgc caccgcctgt cgctgcaatc cagggttcag ctctttttct gagatcatca 241 ccaccccgac ggagacttgt gacgacatca acgagtgtgc aacaccgtcg aaagtgtcat 301 gcggaaaatt ctcggactgc tggaacacag aggggagcta cgactgcgtg tgcagcccgg 361 gatatgagcc tgtttctggg gcaaaaacat tcaagaatga gagcgagaac acctgtcaag 421 atgtggacga gtgcagctcc gggcagcatc agtgtgacag ctccaccgtc tgcttcaaca 481 ccgtgggttc atacagctgc cgctgccgcc caggctggaa gcccagacac ggaatcccga 541 ataaccaaaa ggacactgtc tgtgaagata tgactttctc cacctggacc ccgccccctg 601 gagtccacag ccagacgctt tcccgattct tcgacaaagt ccaggacctg ggcagagact 661 ccaagacaag ctcagccgag gtcaccatcc agaatgtcat caaattggtg gatgaactga 721 tggaagctcc tggagacgta gaggccctgg cgccacctgt ccggcacctc atagccaccc 781 agctgctctc aaaccttgaa gatatcatga ggatcctggc caagagcctg cctaaaggcc 841 ccttcaccta catttcccct tcgaacacag agctgaccct gatgatccag gagcgggggg 901 acaagaacgt cactatgggt cagagcagcg cacgcatgaa gctgaattgg gctgtggcag 961 ctggagccga ggatccaggc cccgccgtgg cgggcatcct ctccatccag aacatgacga 1021 cattgctggc caatgcctcc ttgaacctgc attccaagaa gcaagccgaa ctggaggaga 1081 tatatgaaag cagcatccgt ggtgtccaac tcagacgcct ctctgccgtc aactccatct 1141 ttctgagcca caacaacacc aaggaactca actcccccat ccttttcgcc ttctcccacc 1201 ttgagtcctc cgatggggag gcgggaagag accctcctgc caaggacgtg atgcctgggc 1261 cacggcagga gctgctctgt gccttctgga agagtgacag cgacagggga gggcactggg 1321 ccaccgaggg ctgccaggtg ctgggcagca agaacggcag caccacctgc caatgcagcc 1381 acctgagcag ctttgcgatc cttatggctc attatgacgt ggaggactgg aagctgaccc 1441 tgatcaccag ggtgggactg gcgctgtcac tcttctgcct gctgctgtgc atcctcactt 1501 tcctgctggt gcggcccatc cagggctcgc gcaccaccat acacctgcac ctctgcatct 1561 gcctcttcgt gggctccacc atcttcctgg ccggcatcga gaacgaaggc ggccaggtgg 1621 ggctgcgctg ccgcctggtg gccgggctgc tgcactactg tttcctggcc gccttctgct 1681 ggatgagcct cgaaggcctg gagctctact ttcttgtggt gcgcgtgttc caaggccagg 1741 gcctgagtac gcgctggctc tgcctgatcg gctatggcgt gcccctgctc atcgtgggcg 1801 tctcggctgc catctacagc aagggctacg gccgccccag atactgctgg ttggactttg 1861 agcagggctt cctctggagc ttcttgggac ctgtgacctt CATCATTTTG TGCAATGCTG 1921 TCATTTTCGT GACTACCGTC TGGAAGCTCA CTCAGAAGTT TTCTGAAATC AATCCAGACA 1981 TGAAGAAATT AAAGAAGGCG AGGGCGCTGA CCATCACGGC CATCGCGCAG CTCTTCCTGT 2041 TGGGCTGCAC CTGGGTCTTT GGCCTGTTCA TCTTCGACGA TCGGAGCTTG GTGCTGACCT 2101 ATGTGTTTAC CATCCTCAAC TGCCTGCAGG GCGCCTTCCT CTACCTGCTG CACTGCCTGC 2161 TCAACAAGAA GGTTCGGGAA GAATACCGGA AGTGGGCCTG CCTAGTTGCT GGGGGGAGCA 2221 AGTACTCAGA ATTCACCTCC ACCACGTCTG GCACTGGCCA CAATCAGACC CGGGCCCTCA 2281 GGGCATCAGA GTCCGGCATA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2341 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2401 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCGC CGAGATCCTT 2461 TGTAATTGGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt