Construct: ORF TRCN0000489820
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021608.1_s317c1
- DNA Barcode:
- TGTTTTAACCTCATGTTACAAAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KAT2A (2648)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489820
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2648 | KAT2A | lysine acetyltransferase 2A | NM_021078.3 | 100% | 100% | |
| 2 | human | 2648 | KAT2A | lysine acetyltransferase 2A | XM_006721817.4 | 99.8% | 99.8% | 2019_2021delGGT |
| 3 | human | 2648 | KAT2A | lysine acetyltransferase 2A | XM_017024448.1 | 56.8% | 56.8% | 0_1ins1083 |
| 4 | human | 2648 | KAT2A | lysine acetyltransferase 2A | XM_006721818.4 | 56.8% | 56.8% | 0_1ins1083;936_938delGGT |
| 5 | mouse | 14534 | Kat2a | K(lysine) acetyltransferase 2A | NM_001038010.2 | 89.8% | 97.2% | (many diffs) |
| 6 | mouse | 14534 | Kat2a | K(lysine) acetyltransferase 2A | NM_020004.5 | 89.6% | 97.1% | (many diffs) |
| 7 | mouse | 14534 | Kat2a | K(lysine) acetyltransferase 2A | XM_017314271.1 | 86.7% | 93.9% | (many diffs) |
| 8 | mouse | 14534 | Kat2a | K(lysine) acetyltransferase 2A | XM_017314270.1 | 86.6% | 93.8% | (many diffs) |
| 9 | mouse | 14534 | Kat2a | K(lysine) acetyltransferase 2A | XM_017314273.1 | 49.5% | 54% | (many diffs) |
| 10 | mouse | 14534 | Kat2a | K(lysine) acetyltransferase 2A | XM_017314272.1 | 49.5% | 53.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2580
- ORF length:
- 2511
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcggaacct tcccaggccc cgaccccggc cccggctgcg cagccccggc 121 cccttcagtc cccagcccct gccccaactc cgactcctgc acccagcccg gcttcagccc 181 cgattccgac tcccaccccg gcaccagccc ctgccccagc tgcagcccca gccggcagca 241 cagggactgg ggggcccggg gtaggaagtg ggggggccgg gagcgggggg gatccggctc 301 gacctggcct gagccagcag cagcgcgcca gtcagaggaa ggcgcaagtc cgggggctgc 361 cgcgcgccaa gaagcttgag aagctagggg tcttctcggc ttgcaaggcc aatgaaacct 421 gtaagtgtaa tggctggaaa aaccccaagc cccccactgc accccgcatg gatctgcagc 481 agccagctgc caacctgagt gagctgtgcc gcagttgtga gcaccccttg gctgaccacg 541 tatcccactt ggagaatgtg tcagaggatg agataaaccg actgctgggg atggtggtgg 601 atgtggagaa tctcttcatg tctgttcaca aggaagagga cacagacacc aagcaggtct 661 atttctacct cttcaagcta ctgcggaaat gcatcctgca gatgacccgg cctgtggtgg 721 aggggtccct gggcagccct ccatttgaga aacctaatat tgagcagggt gtgctgaact 781 ttgtgcagta caagtttagt cacctggctc cccgggagcg gcagacgatg ttcgagctct 841 caaagatgtt cttgctctgc cttaactact ggaagcttga gacacctgcc cagtttcggc 901 agaggtctca ggctgaggac gtggctacct acaaggtcaa ttacaccaga tggctctgtt 961 actgccacgt gccccagagc tgtgatagcc tcccccgcta cgaaaccact catgtctttg 1021 ggcgaagcct tctccggtcc attttcaccg ttacccgccg gcagctgctg gaaaagttcc 1081 gagtggagaa ggacaaattg gtgcccgaga agaggaccct catcctcact cacttcccca 1141 aattcctgtc catgctggag gaggagatct atggggcaaa ctctccaatc tgggagtcag 1201 gcttcaccat gccaccctca gaggggacac agctggttcc ccggccagct tcagtcagtg 1261 cagcggttgt tcccagcacc cccatcttca gccccagcat gggtgggggc agcaacagct 1321 ccctgagtct ggattctgca ggggccgagc ctatgccagg cgagaagagg acgctcccag 1381 agaacctgac cctggaggat gccaagcggc tccgtgtgat gggtgacatc cccatggagc 1441 tggtcaatga ggtcatgctg accatcactg accctgctgc catgctgggg cctgagacga 1501 gcctgctttc ggccaatgcg gcccgggatg agacagcccg cctggaggag cgccgcggca 1561 tcatcgagtt ccatgtcatc ggcaactcac tgacgcccaa ggccaaccgg cgggtgttgc 1621 tgtggctcgt ggggctgcag aatgtctttt cccaccagct gccgcgcatg cctaaggagt 1681 atatcgcccg cctcgtcttt gacccgaagc acaagactct ggccttgatc aaggatgggc 1741 gggtcatcgg tggcatctgc ttccgcatgt ttcccaccca gggcttcacg gagattgtct 1801 tctgtgctgt cacctcgaat gagcaggtca agggttatgg gacccacctg atgaaccacc 1861 tgaaggagta tcacatcaag cacaacattc tctacttcct cacctacgcc gacgagtacg 1921 ccatcggcta cttcaaaaag cagggtttct ccaaggacat caaggtgccc aagagccgct 1981 acctgggcta catcaaggac tacgagggag cgacgctgat ggagtgtgag ctgaatcccc 2041 gcatccccta cacggagctg tcccacatca tcaagaagca gaaagagatc atcaagaagc 2101 tgattgagcg caaacaggcc cagatccgca aggtctaccc ggggctcagc tgcttcaagg 2161 agggcgtgag gcagatccct gtggagagcg ttccTGGCAT TCGAGAGACA GGCTGGAAGC 2221 CATTGGGGAA GGAGAAGGGG AAGGAGCTGA AGGACCCCGA CCAGCTCTAC ACAACCCTCA 2281 AAAACCTGCT GGCCCAAATC AAGTCTCACC CCAGTGCCTG GCCCTTCATG GAGCCTGTGA 2341 AGAAGTCGGA GGCCCCTGAC TACTACGAGG TCATCCGCTT CCCCATTGAC CTGAAGACCA 2401 TGACTGAGCG GCTGCGAAGC CGCTACTACG TGACCCGGAA GCTCTTTGTG GCCGACCTGC 2461 AGCGGGTCAT CGCCAACTGT CGCGAGTACA ACCCCCCGGA CAGCGAGTAC TGCCGCTGTG 2521 CCAGCGCCCT GGAGAAGTTC TTCTACTTCA AGCTCAAGGA GGGAGGCCTC ATTGACAAGT 2581 AGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2641 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2701 GGCTTTATAT ATCTTGTGGA AAGGACGATG TTTTAACCTC ATGTTACAAA GTACGCGTTA 2761 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt