Construct: ORF TRCN0000489821
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021215.1_s317c1
- DNA Barcode:
- GGGCGTACGCGACCCTGCACCCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RAMP3 (10268)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489821
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10268 | RAMP3 | receptor activity modifying... | NM_005856.3 | 100% | 100% | |
| 2 | human | 10268 | RAMP3 | receptor activity modifying... | XM_017011666.1 | 78.3% | 78.3% | 0_1ins96 |
| 3 | human | 10268 | RAMP3 | receptor activity modifying... | XM_006715631.3 | 57.1% | 57.1% | 58_390del |
| 4 | mouse | 56089 | Ramp3 | receptor (calcitonin) activ... | XM_006514761.3 | 67.1% | 69.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 516
- ORF length:
- 444
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagact ggagcgctgc ggcgcccgca acttctcccg ttgctgctgc 121 tgctctgcgg tgggtgtccc agagcaggcg gctgcaacga gacaggcatg ttggagaggc 181 tgccccTGTG TGGGAAGGCT TTCGCAGACA TGATGGGCAA GGTGGACGTC TGGAAGTGGT 241 GCAACCTGTC CGAGTTCATC GTGTACTATG AGAGTTTCAC CAACTGCACC GAGATGGAGG 301 CCAATGTCGT GGGCTGCTAC TGGCCCAACC CCCTGGCCCA GGGCTTCATC ACCGGCATCC 361 ACAGGCAGTT CTTCTCCAAC TGCACCGTGG ACAGGGTCCA CTTGGAGGAC CCCCCAGACG 421 AGGTTCTCAT CCCGCTGATC GTTATACCCG TCGTTCTGAC TGTCGCCATG GCTGGCCTGG 481 TGGTGTGGCG CAGCAAACGC ACCGACACGC TGCTGTGAGA CCCAGCTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGAGGGCGT ACGCGACCCT GCACCCTTAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt