Construct: ORF TRCN0000489826
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020777.1_s317c1
- DNA Barcode:
- CCAATCAAACTGCCATCTCCTAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GPR34 (2857)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489826
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2857 | GPR34 | G protein-coupled receptor 34 | NM_001097579.2 | 99.9% | 100% | 960T>C |
| 2 | human | 2857 | GPR34 | G protein-coupled receptor 34 | NM_005300.4 | 99.9% | 100% | 960T>C |
| 3 | human | 2857 | GPR34 | G protein-coupled receptor 34 | XM_005272597.4 | 99.9% | 100% | 960T>C |
| 4 | mouse | 23890 | Gpr34 | G protein-coupled receptor 34 | NM_011823.4 | 85.5% | 89.5% | (many diffs) |
| 5 | mouse | 23890 | Gpr34 | G protein-coupled receptor 34 | XM_006527624.3 | 85.5% | 89.5% | (many diffs) |
| 6 | mouse | 23890 | Gpr34 | G protein-coupled receptor 34 | XM_006527625.3 | 85.5% | 89.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1215
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagaagt cataccataa caatgacgac aacttcagtc agcagctggc 121 cttactcctc ccacagaatg cgctttataa ccaatcatag cgaccaaccg ccacaaaact 181 tctcagcaac accaaatgtt actacctgtc ccatggatga aaaattgcta tctactgtgt 241 taaccacatc ctactctgtt attttcatcg tgggactggt tgggaacata atcgccctct 301 atgtatttct gggtattcac cgtaaaagaa attccattca aatttatcta cttaacgtag 361 ccattgcaga cctcctactc atcttctgcc tccctttccg aataatgtat catattaacc 421 aaaacaagtg gacactaggt gtgattctgt gcaaggttgt gggaacactg ttttatatga 481 acatgtacat tagcattatt ttgcttggat tcatcagttt ggatcgctat ataaaaatta 541 atcggtctat acagcaacgg aaggcaataa caaccaaaca aagtatttat gtctgttgta 601 tagtatggat gcttgctctt ggtggattcc taactatgat tattttaaca cttaagaaag 661 gagggcataa ttccacaatg tgtttccatt acagagataa gcataacgca aaaggagaag 721 ccatttttaa cttcattctt gtggtaatgt tctggctaat tttcttacta ataatccttt 781 catatattaa gattgggaag aatctattGA GGATTTCTAA AAGGAGGTCA AAATTTCCTA 841 ATTCTGGTAA ATATGCCACT ACAGCTCGTA ACTCCTTTAT TGTACTTATC ATTTTTACTA 901 TATGTTTTGT TCCCTATCAT GCCTTTCGAT TCATCTACAT TTCTTCACAG CTAAATGTAT 961 CATCTTGCTA CTGGAAAGAA ATTGTTCACA AAACCAATGA GATCATGCTG GTTCTCTCAT 1021 CTTTCAATAG CTGCTTAGAT CCAGTCATGT ATTTCCTGAT GTCCAGTAAC ATTCGCAAAA 1081 TAATGTGCCA ACTTCTTTTT AGACGATTTC AAGGTGAACC AAGTAGGAGT GAAAGCACTT 1141 CAGAATTTAA ACCAGGATAC TCCCTGCATG ATACATCTGT GGCAGTGAAA ATACAGTCTA 1201 GTTCTAAAAG TACTTAGGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1261 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1321 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCAATCA AACTGCCATC 1381 TCCTAGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt