Construct: ORF TRCN0000489829
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020308.1_s317c1
- DNA Barcode:
- GTTAATTGCGCAACGATCCTCGGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GPN2 (54707)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489829
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54707 | GPN2 | GPN-loop GTPase 2 | NM_018066.4 | 99.8% | 99.6% | 790A>G |
| 2 | mouse | 100210 | Gpn2 | GPN-loop GTPase 2 | NM_133884.2 | 91% | 96.4% | (many diffs) |
| 3 | mouse | 100210 | Gpn2 | GPN-loop GTPase 2 | NM_001290742.1 | 84.3% | 89.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1002
- ORF length:
- 930
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcaggg gccgctccga ccacggcctt cgggcaggcg gtgatcggcc 121 cgccgggctc agggaagacc acgtactgcc tgggcatgag tgagttcctg cgcgcgctgg 181 gccggcgcgt ggcggtggtg aacctggacc cggccaacga ggggctgccg tacgagtgtg 241 ccgtggacgt gggcgagctg gtggggctgg gcgacgtgat ggacgcgctg cgcctggggc 301 ccaacggcgg cctgctctac tgcatggagt acctggaagc caacctggac tggctgcgtg 361 ccaagctcga ccccctccgc ggccactact tcctcttcga ctgcccaggc caggtggagc 421 tctgcacgca tcacggcgcc ttgcgcagca tcttctccca aatggcgcag tgggacctca 481 ggctgactgc cgtccacctc gtggattctc actactgcac agaccctgcc aagttcattt 541 cagtactgtg tacctccctg gccaccatgc tgcacgtgga actgccccac atcaacctcc 601 tttccaagat ggacctcatt gagcattatg ggaagctggc cttcaaccTG GACTACTACA 661 CAGAGGTTCT GGACCTCTCC TACCTGCTTG ACCACCTGGC TTCTGACCCT TTCTTCCGCC 721 ACTACCGCCA GCTCAATGAG AAGCTAGTGC AGCTCATCGA AGACTATAGC CTTGTCTCCT 781 TTATCCCTCT CAACATCCAG GACAAGGAGA GCATCCAGCG AGTCCTGCAG GCTGTGGATA 841 AAGCCAATGG ATACTGTTTC GGAGCCCAAG AGCAGCGAAG CTTGGAAGCC ATGATGTCTG 901 CCGCAATGGG AGCCGACTTC CATTTCTCTT CCACACTGGG CATCCAGGAG AAGTACCTGG 961 CACCCTCGAA CCAGTCAGTG GAGCAGGAAG CCATGCAGCT GTGAGACCCA GCTTTCTTGT 1021 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1081 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1141 GAAAGGACGA GTTAATTGCG CAACGATCCT CGGGACGCGT TAAGTCgaca atcaacctct 1201 ggattacaaa atttgtgaaa gatt