Construct: ORF TRCN0000489839

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021804.1_s317c1
DNA Barcode:
TTCACACCGTTTACACATAAATGC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
SLC22A4 (6583)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489839
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6583 SLC22A4 solute carrier family 22 me... NM_003059.3 99.9% 100% 1233G>C
2 human 6583 SLC22A4 solute carrier family 22 me... XM_011543589.2 83.2% 75.7% 391_392ins104;547_548ins172;957G>C
3 human 6583 SLC22A4 solute carrier family 22 me... XM_006714675.4 67.9% 68% 0_1ins528;705G>C
4 human 6583 SLC22A4 solute carrier family 22 me... XM_017009776.1 67.9% 68% 0_1ins528;705G>C
5 mouse 30805 Slc22a4 solute carrier family 22 (o... NM_019687.4 86.5% 84.8% (many diffs)
6 mouse 30805 Slc22a4 solute carrier family 22 (o... NM_001330304.1 58.3% 57.5% (many diffs)
7 mouse 30805 Slc22a4 solute carrier family 22 (o... XM_006533512.2 58.3% 57.5% (many diffs)
8 mouse 30805 Slc22a4 solute carrier family 22 (o... XM_006533513.1 58.3% 57.5% (many diffs)
9 mouse 30805 Slc22a4 solute carrier family 22 (o... XM_011249065.1 58.3% 57.5% (many diffs)
10 mouse 30805 Slc22a4 solute carrier family 22 (o... XM_017314588.1 58.3% 57.5% (many diffs)
11 mouse 30805 Slc22a4 solute carrier family 22 (o... XM_006533514.3 55% 50.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1722
ORF length:
1653
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gcgggactac gacgaggtga tcgccttcct gggcgagtgg gggcccttcc
121agcgcctcat cttcttcctg ctcagcgcca gcatcatccc caatggcttc aatggtatgt
181cagtcgtgtt cctggcgggg accccggagc accgctgtcg agtgccggac gccgcgaacc
241tgagcagcgc ctggcgcaac aacagtgtcc cgctgcggct gcgggacggc cgcgaggtgc
301cccacagctg cagccgctac cggctcgcca ccatcgccaa cttctcggcg ctcgggctgg
361agccggggcg cgacgtggac ctggggcagc tggagcagga gagctgcctg gatggctggg
421agttcagcca ggacgtctac ctgtccaccg tcgtgaccga gtggaatctg gtgtgtgagg
481acaactggaa ggtgcccctc accacctccc tgttcttcgt aggcgtgctc ctcggctcct
541tcgtgtccgg gcagctgtca gacaggtttg gcaggaagaa cgttctcttc gcaaccatgg
601ctgtacagac tggcttcagc ttcctgcaga ttttctccat cagctgggag atgttcactg
661tgttatttgt catcgtgggc atgggccaga tctccaacta tgtggtagcc ttcatactag
721gaacagaaat tcttggcaag tcagttcgta ttatattctc tacattagga gtgtgcacat
781tttttgcagt tggctatatg ctgctgccac tgtttgctta cttcatcaga gactggcgga
841tgctgctgct ggcgctgacg gtgccgggag tgctgtgtgt cccgctgtgg tggttcattc
901ctgaatctcc ccgatggctg atatcccaga gaagatttag agaggctgaa gatatcatcc
961aaaaagctgc aaaaatgaac aacatagctg taccagcagt gatatttgat tctgtggagg
1021agctaaatcc cctgaagcag cagaaagctt tcattctgga cctgttcagg actcggaata
1081ttgccataat gaccattatg tctttgctgc tatggatgct gacctcagtg ggttactttg
1141ctctgtctct ggatgctcct aatttacatg gagatgccta cctgaactgt ttcctctctg
1201ccttgattga aattccagct tacattacag cctggctgct attgcgaacc ctgcccaggc
1261gttatatcat agctgcagta ctgttctggg gaggaggtgt ccttctcttc attcaactgg
1321tacctgtgga ttattacttc ttatccattg GTCTGGTCAT GCTGGGAAAA TTTGGGATCA
1381CCTCTGCTTT CTCCATGCTG TATGTCTTCA CTGCTGAGCT CTACCCAACC CTGGTCAGGA
1441ACATGGCGGT GGGGGTCACA TCCACGGCCT CCAGAGTGGG CAGCATCATT GCCCCCTACT
1501TTGTTTACCT CGGTGCTTAC AACAGAATGC TGCCCTACAT CGTCATGGGT AGTCTGACTG
1561TCCTGATTGG AATCCTCACC CTTTTTTTCC CTGAAAGTTT GGGAATGACT CTTCCAGAAA
1621CCTTAGAGCA GATGCAGAAA GTGAAATGGT TCAGATCTGG GAAAAAAACA AGAGACTCAA
1681TGGAGACAGA AGAAAATCCC AAGGTTCTAA TAACTGCATT CTGAGACCCA GCTTTCTTGT
1741ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1801AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1861GAAAGGACGA TTCACACCGT TTACACATAA ATGCACGCGT TAAGTCgaca atcaacctct
1921ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)

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