Construct: ORF TRCN0000489839
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021804.1_s317c1
- DNA Barcode:
- TTCACACCGTTTACACATAAATGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC22A4 (6583)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489839
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6583 | SLC22A4 | solute carrier family 22 me... | NM_003059.3 | 99.9% | 100% | 1233G>C |
| 2 | human | 6583 | SLC22A4 | solute carrier family 22 me... | XM_011543589.2 | 83.2% | 75.7% | 391_392ins104;547_548ins172;957G>C |
| 3 | human | 6583 | SLC22A4 | solute carrier family 22 me... | XM_006714675.4 | 67.9% | 68% | 0_1ins528;705G>C |
| 4 | human | 6583 | SLC22A4 | solute carrier family 22 me... | XM_017009776.1 | 67.9% | 68% | 0_1ins528;705G>C |
| 5 | mouse | 30805 | Slc22a4 | solute carrier family 22 (o... | NM_019687.4 | 86.5% | 84.8% | (many diffs) |
| 6 | mouse | 30805 | Slc22a4 | solute carrier family 22 (o... | NM_001330304.1 | 58.3% | 57.5% | (many diffs) |
| 7 | mouse | 30805 | Slc22a4 | solute carrier family 22 (o... | XM_006533512.2 | 58.3% | 57.5% | (many diffs) |
| 8 | mouse | 30805 | Slc22a4 | solute carrier family 22 (o... | XM_006533513.1 | 58.3% | 57.5% | (many diffs) |
| 9 | mouse | 30805 | Slc22a4 | solute carrier family 22 (o... | XM_011249065.1 | 58.3% | 57.5% | (many diffs) |
| 10 | mouse | 30805 | Slc22a4 | solute carrier family 22 (o... | XM_017314588.1 | 58.3% | 57.5% | (many diffs) |
| 11 | mouse | 30805 | Slc22a4 | solute carrier family 22 (o... | XM_006533514.3 | 55% | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1722
- ORF length:
- 1653
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcgggactac gacgaggtga tcgccttcct gggcgagtgg gggcccttcc 121 agcgcctcat cttcttcctg ctcagcgcca gcatcatccc caatggcttc aatggtatgt 181 cagtcgtgtt cctggcgggg accccggagc accgctgtcg agtgccggac gccgcgaacc 241 tgagcagcgc ctggcgcaac aacagtgtcc cgctgcggct gcgggacggc cgcgaggtgc 301 cccacagctg cagccgctac cggctcgcca ccatcgccaa cttctcggcg ctcgggctgg 361 agccggggcg cgacgtggac ctggggcagc tggagcagga gagctgcctg gatggctggg 421 agttcagcca ggacgtctac ctgtccaccg tcgtgaccga gtggaatctg gtgtgtgagg 481 acaactggaa ggtgcccctc accacctccc tgttcttcgt aggcgtgctc ctcggctcct 541 tcgtgtccgg gcagctgtca gacaggtttg gcaggaagaa cgttctcttc gcaaccatgg 601 ctgtacagac tggcttcagc ttcctgcaga ttttctccat cagctgggag atgttcactg 661 tgttatttgt catcgtgggc atgggccaga tctccaacta tgtggtagcc ttcatactag 721 gaacagaaat tcttggcaag tcagttcgta ttatattctc tacattagga gtgtgcacat 781 tttttgcagt tggctatatg ctgctgccac tgtttgctta cttcatcaga gactggcgga 841 tgctgctgct ggcgctgacg gtgccgggag tgctgtgtgt cccgctgtgg tggttcattc 901 ctgaatctcc ccgatggctg atatcccaga gaagatttag agaggctgaa gatatcatcc 961 aaaaagctgc aaaaatgaac aacatagctg taccagcagt gatatttgat tctgtggagg 1021 agctaaatcc cctgaagcag cagaaagctt tcattctgga cctgttcagg actcggaata 1081 ttgccataat gaccattatg tctttgctgc tatggatgct gacctcagtg ggttactttg 1141 ctctgtctct ggatgctcct aatttacatg gagatgccta cctgaactgt ttcctctctg 1201 ccttgattga aattccagct tacattacag cctggctgct attgcgaacc ctgcccaggc 1261 gttatatcat agctgcagta ctgttctggg gaggaggtgt ccttctcttc attcaactgg 1321 tacctgtgga ttattacttc ttatccattg GTCTGGTCAT GCTGGGAAAA TTTGGGATCA 1381 CCTCTGCTTT CTCCATGCTG TATGTCTTCA CTGCTGAGCT CTACCCAACC CTGGTCAGGA 1441 ACATGGCGGT GGGGGTCACA TCCACGGCCT CCAGAGTGGG CAGCATCATT GCCCCCTACT 1501 TTGTTTACCT CGGTGCTTAC AACAGAATGC TGCCCTACAT CGTCATGGGT AGTCTGACTG 1561 TCCTGATTGG AATCCTCACC CTTTTTTTCC CTGAAAGTTT GGGAATGACT CTTCCAGAAA 1621 CCTTAGAGCA GATGCAGAAA GTGAAATGGT TCAGATCTGG GAAAAAAACA AGAGACTCAA 1681 TGGAGACAGA AGAAAATCCC AAGGTTCTAA TAACTGCATT CTGAGACCCA GCTTTCTTGT 1741 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1801 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1861 GAAAGGACGA TTCACACCGT TTACACATAA ATGCACGCGT TAAGTCgaca atcaacctct 1921 ggattacaaa atttgtgaaa gatt