Construct: ORF TRCN0000489846
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020609.1_s317c1
- DNA Barcode:
- CACTGTCACTTCCGTACACGCGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PTK2B (2185)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489846
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | NM_004103.4 | 99.8% | 100% | (many diffs) |
| 2 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | NM_173174.3 | 99.8% | 100% | (many diffs) |
| 3 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | NM_173176.3 | 99.8% | 100% | (many diffs) |
| 4 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | XM_005273447.4 | 99.8% | 100% | (many diffs) |
| 5 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | XM_011544441.2 | 99.8% | 100% | (many diffs) |
| 6 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | XM_017013214.1 | 99.8% | 100% | (many diffs) |
| 7 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | XM_005273448.5 | 98.6% | 98.8% | (many diffs) |
| 8 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | XM_011544442.3 | 98.5% | 98.7% | (many diffs) |
| 9 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | XM_017013215.1 | 98.5% | 98.7% | (many diffs) |
| 10 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | XM_017013216.2 | 97.3% | 97.5% | (many diffs) |
| 11 | human | 2185 | PTK2B | protein tyrosine kinase 2 beta | NM_173175.2 | 95.6% | 95.8% | (many diffs) |
| 12 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | NM_001162366.1 | 89.2% | 95.4% | (many diffs) |
| 13 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | XM_006518726.3 | 89.2% | 95.4% | (many diffs) |
| 14 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | XM_011244994.2 | 89.2% | 95.4% | (many diffs) |
| 15 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | NM_001162365.1 | 88.9% | 95% | (many diffs) |
| 16 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | XM_017315926.1 | 88.4% | 94.4% | (many diffs) |
| 17 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | XM_017315927.1 | 88.4% | 94.4% | (many diffs) |
| 18 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | XM_017315928.1 | 88.4% | 94.4% | (many diffs) |
| 19 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | XM_017315929.1 | 88.4% | 94.4% | (many diffs) |
| 20 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | NM_172498.3 | 85.3% | 91.2% | (many diffs) |
| 21 | mouse | 19229 | Ptk2b | PTK2 protein tyrosine kinas... | XM_017315930.1 | 84.4% | 90.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3099
- ORF length:
- 3027
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtctggg gtgtccgagc ccctgagtcg agtaaagttg ggcacgttac 121 gccggcctga aggccctgca gagcccatgg tggtggtacc agtagatgtg gaaaaggagg 181 acgtgcgtat cctcaaggtc tgcttctata gcaacagctt caatcctggg aaaaacttca 241 aactggtcaa atgcactgtc cagacggaga tccgggagat catcacctcc atcctgctga 301 gcgggcggat cgggcccaac atccggttgg ctgagtgcta tgggctgagg ctgaagcaca 361 tgaagtccga tgagatccac tggctgcacc cacagatgac agtgggtgag gtgcaggaca 421 agtatgagtg tctgcacgtg gaagccgagt ggaggtatga ccttcaaatc cgctacttgc 481 cagaagactt catggagagc ctgaaggagg acaggaccac gctgctctat ttttaccaac 541 agctccggaa cgactacatg cagcgctacg ccagcaaggt cagcgagggc atggccctgc 601 agctgggctg cctggagctc aggcggttct tcaaggatat gccccacaat gcacttgaca 661 agaagtccaa cttcgagctc ctagaaaagg aagtggggct ggacttgttt ttcccaaagc 721 agatgcagga gaacttaaag cccaaacagt tccggaagat gatccagcag accttccagc 781 agtacgcctc gctcagggag gaggagtgcg tcatgaagtt cttcaacact ctcgccggct 841 tcgccaacat cgaccaggag acctaccgct gtgaactcat tcaaggatgg aacattactg 901 tggacctggt cattggccct aaagggatcc gccagctgac tagtcaggac gcaaagccca 961 cctgcctggc cgagttcaag cagatcaggt ccatcaggtg cctcccgctg gaggagggcc 1021 aggcagtact tcagctgggc attgaaggtg ccccccaggc cttgtccatc aaaacctcat 1081 ccctagcaga ggctgagaac atggctgacc tcatagacgg ctactgccgg ctgcagggtg 1141 agcaccaagg ctctctcatc atccatccta ggaaagatgg tgagaagcgg aacagcctgc 1201 cccagatccc catgctaaac ctggaggccc ggcggtccca cctctcagag agctgcagca 1261 tagagtcaga catctacgca gagattcccg acgaaaccct gcgaaggccc ggaggtccac 1321 agtatggcat tgcccgtgaa gatgtggtcc tgaatcgtat tcttggggaa ggcttttttg 1381 gggaggtcta tgaaggtgtc tacacaaatc ataaagggga gaaaatcaat gtagctgtca 1441 agacctgcaa gaaagactgc actctggaca acaaggagaa gttcatgagc gaggcagtga 1501 tcatgaagaa cctcgaccac ccgcacatcg tgaagctgat cggcatcatt gaagaggagc 1561 ccacctggat catcatggaa ttgtatccct atggggagct gggccactac ctggagcgga 1621 acaagaactc cctgaaggtg ctcaccctcg tgctgtactc actgcagata tgcaaagcca 1681 tggcctacct ggagagcatc aactgcgtgc acagggacat tgctgtccgg aacatcctgg 1741 tggcctcccc tgagtgtgtg aagctggggg actttggtct ttcccggtac attgaggacg 1801 aggactatta caaagcctct gtgactcgtc tccccatcaa atggatgtcc ccagagtcca 1861 ttaacttccg acgcttcacg acagccagtg acgtctggat gttcgccgtg tgcatgtggg 1921 agatcctgag ctttgggaag cagcccttct tctggctgga gaacaaggat gtcatcgggg 1981 tgctggagaa aggagaccgg ctgcccaagc ctgatctctg tccaccggtc ctttataccc 2041 tcatgacccg ctgctgggac tacgacccca gtgaccggcc ccgcttcacc gagctggtgt 2101 gcagcctcag tgacgtttat cagatggaga aggacattgc catggagcaa gagaggaatg 2161 ctcgctaccg aacccccaaa atcttggagc ccacagcctt ccaggaaccc ccacccaagc 2221 ccagccgacc taagtacaga ccccctccgc aaaccaacct cctggctcca aagctgcagt 2281 tccaggttcc tgagggtctg tgtgccagct ctcctacgct caccagccct atggagtatc 2341 catctcccgt taactcactg cacaccccac ctctccaccg gcacaatgtc ttcaaacgcc 2401 acagcatgcg ggaggaggac ttcatccaac ccagcagccg agaagaggcc cagcagctgt 2461 gggaggctga aaaggtcaaa atgcggcaaa tcctggacaa acagcagaag cagatggtgg 2521 aggactacca gtggctcagg caggaggaga agtccctgga ccccatggtt tatatgaatg 2581 ataagtcccc attgacgcca gagaaggagg tcggctacct ggagttcaca gggcccccac 2641 agaagccccc gaggctgggc gcacagtcca tccagcccac agctaacctg gaccggaccg 2701 atgacctggt gtaccTCAAT GTCATGGAGC TGGTGCGGGC CGTGCTGGAG CTCAAGAATG 2761 AGCTCTGTCA GCTGCCCCCC GAGGGCTACG TGGTGGTGGT GAAGAATGTG GGGCTGACCC 2821 TGCGGAAGCT CATCGGGAGC GTGGATGATC TCCTGCCTTC CTTGCCGTCA TCTTCACGGA 2881 CAGAGATCGA GGGCACCCAG AAACTGCTCA ACAAAGACCT GGCAGAGCTC ATCAACAAGA 2941 TGCGGCTGGC GCAGCAGAAC GCCGTGACCT CCCTGAGTGA GGAGTGCAAG AGGCAGATGC 3001 TGACGGCTTC ACACACCCTG GCTGTGGACG CCAAGAACCT GCTCGACGCT GTGGACCAGG 3061 CCAAGGTTCT GGCCAATCTG GCCCACCCAC CTGCAGAGTG AGACCCAGCT TTCTTGTACA 3121 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 3181 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 3241 AGGACGACAC TGTCACTTCC GTACACGCGC CACGCGTTAA GTCgacaatc aacctctgga 3301 ttacaaaatt tgtgaaagat t