Construct: ORF TRCN0000489848
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020239.1_s317c1
- DNA Barcode:
- AAAATTCCTGGGTCCCTCTTAGTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- UCK2 (7371)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489848
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7371 | UCK2 | uridine-cytidine kinase 2 | NM_001363568.2 | 46.2% | 46.2% | 1_387del |
| 2 | human | 7371 | UCK2 | uridine-cytidine kinase 2 | NM_012474.5 | 42.5% | 42.5% | 1_450del |
| 3 | mouse | 80914 | Uck2 | uridine-cytidine kinase 2 | XM_006497040.3 | 43.3% | 47.5% | (many diffs) |
| 4 | mouse | 80914 | Uck2 | uridine-cytidine kinase 2 | NM_030724.3 | 38% | 41.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 96
- ORF end:
- 429
- ORF length:
- 333
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGCAGGCT TCACCATGAA GCTTTTTGTG GATACAGATG 121 CGGACACCCG GCTCTCACGC AGAGTATTAA GGGACATCAG CGAGAGAGGC AGGGATCTTG 181 AGCAGATTTT ATCTCAGTAC ATTACGTTCG TCAAGCCTGC CTTTGAGGAA TTCTGCTTGC 241 CAACAAAGAA GTATGCTGAT GTGATCATCC CTAGAGGTGC AGATAATCTG GTGGCCATCA 301 ACCTCATCGT GCAGCACATC CAGGACATCC TGAATGGAGG GCCCTCCAAA CGGCAGACCA 361 ATGGCTGTCT CAACGGCTAC ACCCCTTCAC GCAAGAGGCA GGCATCGGAG TCCAGCAGCA 421 GGCCGCATTG AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 481 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 541 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAA ATTCCTGGGT CCCTCTTAGT 601 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t