Construct: ORF TRCN0000489858
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021668.1_s317c1
- DNA Barcode:
- ATACCTGAGCCCACGATTAAGAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GYS1 (2997)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489858
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2997 | GYS1 | glycogen synthase 1 | NM_002103.5 | 100% | 100% | |
| 2 | human | 2997 | GYS1 | glycogen synthase 1 | NM_001161587.2 | 91.3% | 91.3% | 298_299ins192 |
| 3 | human | 2997 | GYS1 | glycogen synthase 1 | NR_027763.2 | 56.8% | 1_197del;314_315ins182;2227_3387del | |
| 4 | mouse | 14936 | Gys1 | glycogen synthase 1, muscle | NM_030678.3 | 87.3% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2280
- ORF length:
- 2211
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcctttaaac cgcactttgt ccatgtcctc actgccagga ctggaggact 121 gggaggatga attcgacctg gagaacgcag tgctcttcga agtggcctgg gaggtggcta 181 acaaggtggg tggcatctac acggtgctgc agacgaaggc gaaggtgaca ggggacgaat 241 ggggcgacaa ctacttcctg gtggggccgt acacggagca gggcgtgagg acccaggtgg 301 aactgctgga ggcccccacc ccggccctga agaggacact ggattccatg aacagcaagg 361 gctgcaaggt gtatttcggg cgctggctga tcgagggagg ccctctggtg gtgctcctgg 421 acgtgggtgc ctcagcttgg gccctggagc gctggaaggg agagctctgg gatacctgca 481 acatcggagt gccgtggtac gaccgcgagg ccaacgacgc tgtcctcttt ggctttctga 541 ccacctggtt cctgggtgag ttcctggcac agagtgagga gaagccacat gtggttgctc 601 acttccatga gtggttggca ggcgttggac tctgcctgtg tcgtgcccgg cgactgcctg 661 tagcaaccat cttcaccacc catgccacgc tgctggggcg ctacctgtgt gccggtgccg 721 tggacttcta caacaacctg gagaacttca acgtggacaa ggaagcaggg gagaggcaga 781 tctaccaccg atactgcatg gaaagggcgg cagcccactg cgctcacgtc ttcactactg 841 tgtcccagat caccgccatc gaggcacagc acttgctcaa gaggaaacca gatattgtga 901 cccccaatgg gctgaatgtg aagaagtttt ctgccatgca tgagttccag aacctccatg 961 ctcagagcaa ggctcgaatc caggagtttg tgcggggcca tttttatggg catctggact 1021 tcaacttgga caagacctta tacttcttta tcgccggccg ctatgagttc tccaacaagg 1081 gtgctgacgt cttcctggag gcattggctc ggctcaacta tctgctcaga gtgaacggca 1141 gcgagcagac agtggttgcc ttcttcatca tgccagcgcg gaccaacaat ttcaacgtgg 1201 aaaccctcaa aggccaagct gtgcgcaaac agctttggga cacggccaac acggtgaagg 1261 aaaagttcgg gaggaagctt tatgaatcct tactggttgg gagccttccc gacatgaaca 1321 agatgctgga taaggaagac ttcactatga tgaagagagc catctttgca acgcagcggc 1381 agtctttccc ccctgtgtgc acccacaata tgctggatga ctcctcagac cccatcctga 1441 ccaccatccg ccgaatcggc ctcttcaata gcagtgccga cagggtgaag gtgattttcc 1501 acccggagtt cctctcctcc acaagccccc tgctccctgt ggactatgag gagtttgtcc 1561 gtggctgtca ccttggagtc ttcccctcct actatgagcc ttggggctac acaccggctg 1621 agtgcacggt tatgggaatc cccagtatct ccaccaatct ctccggcttc ggctgcttca 1681 tggaggaaca catcgcagac ccctcagctt acggtatcta cattcttgac cggcggttcc 1741 gcagcctgga tgattcctgc tcgcagctca cctccttcct ctacagtttc tgtcagcaga 1801 gccggcggca gcgtatcatc cagcggaacc gcacggagcg cctctccgac cttctggact 1861 ggaaatacct aggccggtac tatatgtctg cgcgccacat ggcgctgtcc aaggcctttc 1921 cagagcactt caccTACGAG CCCAACGAGG CGGATGCGGC CCAGGGGTAC CGCTACCCAC 1981 GGCCAGCCTC GGTGCCACCG TCGCCCTCGC TGTCACGACA CTCCAGCCCG CACCAGAGTG 2041 AGGACGAGGA GGATCCCCGG AACGGGCCGC TGGAGGAAGA CGGCGAGCGC TACGATGAGG 2101 ACGAGGAGGC CGCCAAGGAC CGGCGCAACA TCCGTGCACC AGAGTGGCCG CGCCGAGCGT 2161 CCTGCACCTC CTCCACCAGC GGCAGCAAGC GCAACTCTGT GGACACGGCC ACCTCCAGCT 2221 CACTCAGCAC CCCGAGCGAG CCCCTCAGCC CCACCAGCTC CCTGGGCGAG GAGCGTAACT 2281 AGAACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2341 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2401 GGCTTTATAT ATCTTGTGGA AAGGACGAAT ACCTGAGCCC ACGATTAAGA ACACGCGTTA 2461 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt