Construct: ORF TRCN0000489869
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022027.1_s317c1
- DNA Barcode:
- AAACGACGTAGCTTTGGCACAGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC29A3 (55315)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489869
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55315 | SLC29A3 | solute carrier family 29 me... | NM_018344.6 | 100% | 100% | |
| 2 | human | 55315 | SLC29A3 | solute carrier family 29 me... | NM_001363518.1 | 83.5% | 83.5% | 0_1ins234 |
| 3 | human | 55315 | SLC29A3 | solute carrier family 29 me... | XM_017016377.2 | 69.2% | 69.2% | 0_1ins438 |
| 4 | human | 55315 | SLC29A3 | solute carrier family 29 me... | NR_033413.1 | 58.9% | 1_57del;356_357ins83;1400_2194del | |
| 5 | human | 55315 | SLC29A3 | solute carrier family 29 me... | XM_017016378.2 | 56.6% | 56.6% | 0_1ins618 |
| 6 | human | 55315 | SLC29A3 | solute carrier family 29 me... | NM_001174098.1 | 54.3% | 54.3% | 774_775ins651 |
| 7 | human | 55315 | SLC29A3 | solute carrier family 29 me... | NR_033414.1 | 48.9% | 1_57del;356_357ins310;1173_1967del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1497
- ORF length:
- 1425
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccgtt gtctcagagg acgactttca gcacagttca aactccacct 121 acagaaccac aagcagcagt ctccgagctg accaggaggc actgcttgag aagctgctgg 181 accgcccgcc ccctggcctg cagaggcccg aggaccgctt ctgtggcaca tacatcatct 241 tcttcagcct gggcattggc agtctactgc catggaactt ctttatcact gccaaggagt 301 actggatgtt caaactccgc aactcctcca gcccagccac cggggaggac cctgagggct 361 cagacatcct gaactacttt gagagctacc ttgccgttgc ctccaccgtg ccctccatgc 421 tgtgcctggt ggccaacttc ctgcttgtca acagggttgc agtccacatc cgtgtcctgg 481 cctcactgac ggtcatcctg gccatcttca tggtgataac tgcactggtg aaggtggaca 541 cttcctcctg gacccgtggc ttttttgcgg tcaccattgt ctgcatggtg atcctcagcg 601 gtgcctccac tgtcttcagc agcagcatct acggcatgac cggctccttt cctatgagga 661 actcccaggc actgatatca ggaggagcca tgggcgggac ggtcagcgcc gtggcctcat 721 tggtggactt ggctgcatcc agtgatgtga ggaacagcgc cctggccttc ttcctgacgg 781 ccactgtctt cctcgtgctc tgcatgggac tctacctgct gctgtccagg ctggagtatg 841 ccaggtacta catgaggcct gttcttgcgg cccatgtgtt ttctggtgaa gaggagcttc 901 cccaggactc cctcagtgcc ccttcggtgg cctccagatt cattgattcc cacacacccc 961 ctctccgccc catcctgaag aagacggcca gcctgggctt ctgtgtcacc tacgtcttct 1021 tcatcaccag cctcatctac cccgccatct gcaccaacat cgagtccctc aacaagggtt 1081 cgggctcact gtggaccacc aagtttttca tccccctcac taccttcctc ctGTACAACT 1141 TTGCTGACCT ATGTGGCCGG CAGCTCACCG CCTGGATCCA GGTGCCAGGG CCCAATAGCA 1201 AGGCGCTCCC AGGGTTCGTG CTCCTCCGGA CCTGCCTCAT CCCCCTCTTC GTGCTCTGTA 1261 ACTACCAGCC CCGCGTCCAC CTGAAGACTG TGGTCTTCCA GTCCGATGTG TACCCCGCAC 1321 TCCTCAGCTC CCTGCTGGGG CTCAGCAACG GCTACCTCAG CACCCTGGCC CTCCTCTACG 1381 GGCCTAAGAT TGTGCCCAGG GAGCTGGCTG AGGCCACGGG AGTGGTGATG TCCTTTTATG 1441 TGTGCTTGGG CTTAACACTG GGCTCAGCCT GCTCTACCCT CCTGGTGCAC CTCATCTAGG 1501 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1561 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1621 TTTATATATC TTGTGGAAAG GACGAAAACG ACGTAGCTTT GGCACAGTGA CGCGTTAAGT 1681 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt