Construct: ORF TRCN0000489870
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021242.1_s317c1
- DNA Barcode:
- CTTTACCTAAGATGTTAGGCTCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DYRK3 (8444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489870
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8444 | DYRK3 | dual specificity tyrosine p... | XM_011510061.2 | 100% | 100% | |
| 2 | human | 8444 | DYRK3 | dual specificity tyrosine p... | NM_001004023.2 | 97.3% | 97.3% | 1_45del |
| 3 | human | 8444 | DYRK3 | dual specificity tyrosine p... | XM_005273315.4 | 97.3% | 97.3% | 1_45del |
| 4 | human | 8444 | DYRK3 | dual specificity tyrosine p... | NM_003582.3 | 94% | 94% | 1_105del |
| 5 | mouse | 226419 | Dyrk3 | dual-specificity tyrosine-(... | XM_006529427.2 | 84.8% | 89.8% | (many diffs) |
| 6 | mouse | 226419 | Dyrk3 | dual-specificity tyrosine-(... | NM_145508.2 | 79.8% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 59
- ORF end:
- 1718
- ORF length:
- 1659
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaccat 61 gatgatagat gaaaccaaat gtcccccctg ttcaaatgta ctctgcaatc cttctgaacc 121 acctccaccc agaagactaa atatgaccac tgagcagttt acaggagatc atactcagca 181 ctttttggat ggaggtgaga tgaaggtaga acagctgttt caagaatttg gcaacagaaa 241 atccaatact attcagtcag atggcatcag tgactctgaa aaatgctctc ctactgtttc 301 tcagggtaaa agttcagatt gcttgaatac agtaaaatcc aacagttcat ccaaggcacc 361 caaagtggtg cctctgactc cagaacaagc cctgaagcaa tataaacacc acctcactgc 421 ctatgagaaa ctggaaataa ttaattatcc agaaatttac tttgtaggtc caaatgccaa 481 gaaaagacat ggagttattg gtggtcccaa taatggaggg tatgatgatg cagatggggc 541 ctatattcat gtacctcgag accatctagc ttatcgatat gaggtgctga aaattattgg 601 caaggggagt tttgggcagg tggccagggt ctatgatcac aaacttcgac agtacgtggc 661 cctaaaaatg gtgcgcaatg agaagcgctt tcatcgtcaa gcagctgagg agatccggat 721 tttggagcat cttaagaaac aggataaaac tggtagtatg aacgttatcc acatgctgga 781 aagtttcaca ttccggaacc atgtttgcat ggcctttgaa ttgctgagca tagaccttta 841 tgagctgatt aaaaaaaata agtttcaggg ttttagcgtc cagttggtac gcaagtttgc 901 ccagtccatc ttgcaatctt tggatgccct ccacaaaaat aagattattc actgcgatct 961 gaagccagaa aacattctcc tgaaacacca cgggcgcagt tcaaccaagg tcattgactt 1021 tgggtccagc tgtttcgagt accagaagct ctacacatat atccagtctc ggttctacag 1081 agctccagaa atcatcttag gaagccgcta cagcacacca attgacatat ggagttttgg 1141 ctgcatcctt gcagaacttt taacaggaca gcctctcttc cctggagagg atgaaggaga 1201 ccagttggcc tgcatgatgg agcttctagg gatgccacca ccaaaacttc tggagcaatc 1261 caaacgtgcc aagtacttta ttaattccaa gggcataccc cgctacTGCT CTGTGACTAC 1321 CCAGGCAGAT GGGAGGGTTG TGCTTGTGGG GGGTCGCTCA CGTAGGGGTA AAAAGCGGGG 1381 TCCCCCAGGC AGCAAAGACT GGGGGACAGC ACTGAAAGGG TGTGATGACT ACTTGTTTAT 1441 AGAGTTCTTG AAAAGGTGTC TTCACTGGGA CCCCTCTGCC CGCTTGACCC CAGCTCAAGC 1501 ATTAAGACAC CCTTGGATTA GCAAGTCTGT CCCCAGACCT CTCACCACCA TAGACAAGGT 1561 GTCAGGGAAA CGGGTAGTTA ATCCTGCAAG TGCTTTCCAG GGATTGGGTT CTAAGCTGCC 1621 TCCAGTTGTT GGAATAGCCA ATAAGCTTAA AGCTAACTTA ATGTCAGAAA CCAATGGTAG 1681 TATACCCCTA TGCAGTGTAT TGCCAAAACT GATTAGCTAG GACCCAGCTT TCTTGTACAA 1741 AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA 1801 ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA 1861 GGACGACTTT ACCTAAGATG TTAGGCTCAT ACGCGTTAAG TCgacaatca acctctggat 1921 tacaaaattt gtgaaagatt