Construct: ORF TRCN0000489874
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019185.1_s317c1
- DNA Barcode:
- TGTGGACACTCCTGTGGGTATCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R14 (50840)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489874
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50840 | TAS2R14 | taste 2 receptor member 14 | NM_023922.1 | 100% | 100% | |
| 2 | human | 106707243 | PRH1-TAS2R14 | PRH1-TAS2R14 readthrough | NM_001316893.2 | 45.2% | 38.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1026
- ORF length:
- 951
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgggt ggtgtcataa agagcatatt tacattcgtt ttaattgtgg 121 aatttataat tggaaattta ggaaatagtt tcatagcact ggtgaactgt attgactggg 181 tcaagggaag aaagatctct tcggttgatc ggatcctcac tgctttggca atctctcgaa 241 ttagcctggt ttggttaata ttcggaagct ggtgtgtgtc tgtgtttttc ccagctttat 301 ttgccactga aaaaatgttc agaatgctta ctaatatctg gacagtgatc aatcatttta 361 gtgtctggtt agctacaggc ctcggtactt tttattttct caagatagcc aatttttcta 421 actctatttt tctctaccta aagtggaggg ttaaaaaggt ggttttggtg ctgcttcttg 481 tgacttcggt cttcttgttt ttaaatattg cactgataaa catccatata aatgccagta 541 tcaatggata cagaagaaac aagacttgca gttctgattc aagtaacttt acacgatttt 601 ccagtcttat tgtattaacc agcactgtgt tcattttcat accctttact ttgtccctgg 661 caatgtttct tctccTCATC TTCTCCATGT GGAAACATCG CAAGAAGATG CAGCACACTG 721 TCAAAATATC CGGAGACGCC AGCACCAAAG CCCACAGAGG AGTTAAAAGT GTGATCACTT 781 TCTTCCTACT CTATGCCATT TTCTCTCTGT CTTTTTTCAT ATCAGTTTGG ACCTCTGAAA 841 GGTTGGAGGA AAATCTAATT ATTCTTTCCC AGGTGATGGG AATGGCTTAT CCTTCATGTC 901 ACTCATGTGT TCTGATTCTT GGAAACAAGA AGCTGAGACA GGCCTCTCTG TCAGTGCTAC 961 TGTGGCTGAG GTACATGTTC AAAGATGGGG AGCCCTCAGG TCACAAAGAA TTTAGAGAAT 1021 CATCTGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1081 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1141 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATGTGGACAC TCCTGTGGGT ATCTTACGCG 1201 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt