Construct: ORF TRCN0000489881
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021351.1_s317c1
- DNA Barcode:
- TCTTCAAACTGACCCGGTAAGATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ACYP2 (98)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489881
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 98 | ACYP2 | acylphosphatase 2 | NM_138448.4 | 100% | 100% | |
| 2 | human | 98 | ACYP2 | acylphosphatase 2 | NM_001320588.2 | 75.7% | 75.7% | 0_1ins72 |
| 3 | human | 98 | ACYP2 | acylphosphatase 2 | NM_001320589.2 | 67.4% | 64.6% | (many diffs) |
| 4 | human | 98 | ACYP2 | acylphosphatase 2 | NM_001320590.2 | 65.3% | 62.6% | (many diffs) |
| 5 | human | 98 | ACYP2 | acylphosphatase 2 | NM_001320587.2 | 64.9% | 60.2% | (many diffs) |
| 6 | human | 98 | ACYP2 | acylphosphatase 2 | NM_001320586.2 | 49.3% | 46% | (many diffs) |
| 7 | human | 98 | ACYP2 | acylphosphatase 2 | XM_017005411.1 | 42.9% | 40% | (many diffs) |
| 8 | human | 98 | ACYP2 | acylphosphatase 2 | XM_017005410.1 | 40.9% | 28.7% | (many diffs) |
| 9 | human | 98 | ACYP2 | acylphosphatase 2 | XM_017005412.1 | 27.3% | 22.9% | (many diffs) |
| 10 | human | 98 | ACYP2 | acylphosphatase 2 | XM_017005413.1 | 25.2% | 22.1% | (many diffs) |
| 11 | human | 98 | ACYP2 | acylphosphatase 2 | XR_001739083.1 | 16.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 390
- ORF length:
- 297
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGCAGGCA CCATGTCTAC CGCCCAGTCA CTCAAATCCG 121 TGGACTACGA GGTGTTCGGA AGAGTGCAGG GTGTTTGCTT CAGAATGTAT ACAGAAGATG 181 AAGCTAGGAA AATAGGAGTG GTTGGCTGGG TGAAGAATAC CAGCAAAGGC ACCGTGACAG 241 GCCAAGTGCA GGGGCCAGAA GACAAAGTCA ATTCCATGAA GTCCTGGCTG AGCAAGGTTG 301 GAAGCCCTAG TTCTCGCATT GACCGCACAA ACTTTTCTAA TGAAAAAACC ATCTCTAAGC 361 TTGAATACTC TAATTTTAGT ATTAGATACT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 421 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 481 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 541 TTCAAACTGA CCCGGTAAGA TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 601 ttgtgaaaga tt