Construct: ORF TRCN0000489882
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021320.1_s317c1
- DNA Barcode:
- GTACGTAGGCTGAATTCCAACGCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RGS13 (6003)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489882
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6003 | RGS13 | regulator of G protein sign... | NM_002927.5 | 100% | 100% | |
| 2 | human | 6003 | RGS13 | regulator of G protein sign... | NM_144766.3 | 100% | 100% | |
| 3 | mouse | 246709 | Rgs13 | regulator of G-protein sign... | NM_153171.4 | 84.9% | 83% | (many diffs) |
| 4 | mouse | 246709 | Rgs13 | regulator of G-protein sign... | XM_006529613.2 | 84.9% | 83% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 549
- ORF length:
- 477
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagcagg cggaattgtt ggatttgtaa gatgtgcaga gatgaatcta 121 agaggccccc ttcaaacctt actttggagg aagtattaca gtgggcccag tcttttgaaa 181 atttaatGGC TACAAAATAT GGTCCAGTAG TCTATGCAGC ATATTTAAAA ATGGAGCACA 241 GTGACGAGAA TATTCAATTC TGGATGGCAT GTGAAACCTA TAAGAAAATT GCCTCACGGT 301 GGAGCAGAAT TTCTAGGGCA AAGAAGCTTT ATAAGATTTA CATCCAGCCA CAGTCCCCTA 361 GAGAGATTAA CATTGACAGT TCGACAAGAG AGACTATCAT CAGGAACATT CAGGAACCCA 421 CTGAAACATG TTTTGAAGAA GCTCAGAAAA TAGTCTATAT GCATATGGAA AGGGATTCCT 481 ACCCCAGATT TCTAAAGTCA GAAATGTACC AAAAACTTTT GAAAACTATG CAGTCCAACA 541 ACAGTTTCTA GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTA CGTAGGCTGA ATTCCAACGC 721 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t