Construct: ORF TRCN0000489890
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021355.1_s317c1
- DNA Barcode:
- CTGGCACTGACCGGGTAGTTTCTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRSS2 (5645)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489890
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5645 | PRSS2 | serine protease 2 | NM_002770.4 | 96.7% | 96.7% | 718_741del |
| 2 | human | 5645 | PRSS2 | serine protease 2 | NM_001303414.1 | 91.5% | 91.5% | 201_242del;760_783del |
| 3 | human | 5646 | PRSS3 | serine protease 3 | NM_002771.3 | 90.3% | 85.4% | (many diffs) |
| 4 | human | 5644 | PRSS1 | serine protease 1 | NM_002769.5 | 89.8% | 86.6% | (many diffs) |
| 5 | human | 5646 | PRSS3 | serine protease 3 | NM_001197098.1 | 86.1% | 82.1% | (many diffs) |
| 6 | human | 5646 | PRSS3 | serine protease 3 | NM_001197097.2 | 83.9% | 77.7% | (many diffs) |
| 7 | human | 5645 | PRSS2 | serine protease 2 | NR_130149.2 | 79.4% | 1_13del;52_53ins74;657_735del | |
| 8 | human | 154754 | PRSS3P2 | PRSS3 pseudogene 2 | NR_001296.3 | 77.7% | (many diffs) | |
| 9 | human | 5646 | PRSS3 | serine protease 3 | NM_007343.3 | 72.4% | 68% | (many diffs) |
| 10 | human | 5646 | PRSS3 | serine protease 3 | XM_011517965.1 | 65.3% | 60.9% | (many diffs) |
| 11 | human | 5644 | PRSS1 | serine protease 1 | XM_011516411.1 | 47% | 45.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 789
- ORF length:
- 717
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaatcta cttctgatcc ttacctttgt tgcagctgct gttgctgccc 121 cctttgatga tgatgacaag atcgttgggg gctacatctg tgaggagaat tctgtcccct 181 accaggtgtc cttgaattct ggctaccact tctgcggtgg ctccctcatc agcgaacagt 241 gggtggtgtc agcaggtcac tgctacaagt cccgcatcca ggtgagactg ggagagcaca 301 acatcgaagt cctggagggg aatgaaCAGT TCATCAATGC GGCCAAGATC ATCCGCCACC 361 CCAAATACAA CAGCCGGACT CTGGACAATG ACATCCTGCT GATCAAGCTC TCCTCACCTG 421 CCGTCATCAA TTCCCGCGTG TCCGCCATCT CTCTGCCCAC TGCCCCTCCA GCTGCTGGCA 481 CCGAGTCCCT CATCTCCGGC TGGGGCAACA CTCTGAGTTC TGGTGCCGAC TACCCAGACG 541 AGCTGCAGTG CCTGGATGCT CCTGTGCTGA GCCAGGCTGA GTGTGAAGCC TCCTACCCTG 601 GAAAGATTAC CAACAACATG TTCTGTGTGG GCTTCCTCGA GGGAGGCAAG GATTCCTGCC 661 AGGGTGATTC TGGTGGCCCT GTGGTCTCCA ATGGAGAGCT CCAAGGAATT GTCTCCTGGG 721 GCTATGGCTG TGCCCAGAAG AACAGGCCTG GAGTCTACAC CAAGGTCTAC AACTATGTGG 781 ACTGGATTTA GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 841 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 901 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTG GCACTGACCG GGTAGTTTCT 961 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t