Construct: ORF TRCN0000489902
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019995.1_s317c1
- DNA Barcode:
- AAAGTGCGTTAACTGTCTGCGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAQR8 (85315)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489902
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85315 | PAQR8 | progestin and adipoQ recept... | NM_133367.5 | 99.7% | 99.1% | 53A>G;598C>T;1062_1063insG |
| 2 | mouse | 74229 | Paqr8 | progestin and adipoQ recept... | NM_028829.3 | 86.3% | 93.2% | (many diffs) |
| 3 | mouse | 74229 | Paqr8 | progestin and adipoQ recept... | XM_006495577.2 | 86.3% | 93.2% | (many diffs) |
| 4 | mouse | 74229 | Paqr8 | progestin and adipoQ recept... | XM_006495578.3 | 86.3% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1137
- ORF length:
- 1065
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgacgacc gccatcttgg agcgcctgag caccctgtcg gtcagcgggc 121 agcggctgcg ccgcctgccc aagatcctgg aggatgggct tcccaagatg ccttgcactg 181 tcccagaaac ggatgtgccc cagctcttcc gggagcctta catccgcacc ggctaccgcc 241 ccacggggca cgagtggcgc tactacttct tcagcctctt tcagaaacac aacgaggtgg 301 tcaacgtctg gacccattta ctggcagccc tggccgtcct cttgcgattc tgggcctttg 361 ccgaggctga ggccttgcca tgggcgtcta cccactccct gcctctgctc ctcttcatcc 421 tgtcgtcaat cacttacctc acctgcagcc ttctggccca cctgctgcag tccaagtcag 481 agctctccca ctacaccttc tactttgtgg actatgttgg cgtgagcgtt taccaatatg 541 gcagtgcttt ggctcatttc ttctacagct ctgaccaggc ctggtatgac cggttctggc 601 ttttcttctt gccagcagct gccttctgtg gctggttatc ttgtgctggc tgttgctatg 661 ccaaatattg ttaccggagg ccttatccag tcatgaggaa gatctgtcaa gtggtgccag 721 caggtctggc ttttatccta gacatcagcc ctgtggcaca ccgtgtggcg ctctgTCACC 781 TGGCTGGCTG CCAGGAGCAA GCAGCCTGGT ACCACACCCT CCAGATCCTC TTCTTCCTGG 841 TTAGCGCTTA TTTCTTCTCC TGCCCCGTGC CTGAGAAGTA CTTCCCGGGT TCCTGTGACA 901 TCGTGGGCCA TGGGCATCAG ATCTTCCATG CATTTCTGTC CATCTGTACG CTCTCCCAGC 961 TGGAGGCCAT CCTCCTGGAC TACCAGGGGC GGCAGGAGAT CTTCCTGCAG CGCCATGGAC 1021 CCCTATCTGT CCACATGGCC TGCCTCTCCT TCTTCTTCCT GGCTGCCTGC AGTGCTGCCA 1081 CCGCAGCCCT TCTGAGGCAC AAAGTCAAGG CCAGACTGAC CAAGAAAGAT TCCGACCCAG 1141 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1201 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1261 TATCTTGTGG AAAGGACGAA AAGTGCGTTA ACTGTCTGCG ACCACGCGTT AAGTCgacaa 1321 tcaacctctg gattacaaaa tttgtgaaag att