Construct: ORF TRCN0000489915
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021615.1_s317c1
- DNA Barcode:
- GCAGCTTCCAAACTCTGAACACTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ALDH1A3 (220)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489915
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 220 | ALDH1A3 | aldehyde dehydrogenase 1 fa... | NM_000693.4 | 99.9% | 100% | 1326C>T |
| 2 | human | 220 | ALDH1A3 | aldehyde dehydrogenase 1 fa... | NM_001293815.2 | 79% | 79.1% | 345_346ins321;1005C>T |
| 3 | mouse | 56847 | Aldh1a3 | aldehyde dehydrogenase fami... | NM_053080.3 | 87.2% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1605
- ORF length:
- 1536
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggccaccgct aacggggccg tggaaaacgg gcagccggac aggaagccgc 121 cggccctgcc gcgccccatc cgcaacctgg aggtcaagtt caccaagata tttatcaaca 181 atgaatggca cgaatccaag agtgggaaaa agtttgctac atgtaaccct tcaactcggg 241 agcaaatatg tgaagtggaa gaaggagata agcccgacgt ggacaaggct gtggaggctg 301 cacaggttgc cttccagagg ggctcgccat ggcgccggct ggatgccctg agtcgtgggc 361 ggctgctgca ccagctggct gacctggtgg agagggaccg cgccaccttg gccgccctgg 421 agacgatgga tacagggaag ccatttcttc atgctttttt catcgacctg gagggctgta 481 ttagaaccct cagatacttt gcagggtggg cagacaaaat ccagggcaag accatcccca 541 cagatgacaa cgtcgtgtgc ttcaccaggc atgagcccat tggtgtctgt ggggccatca 601 ctccatggaa cttccccctg ctgatgctgg tgtggaagct ggcacccgcc ctctgctgtg 661 ggaacaccat ggtcctgaag cctgcggagc agacacctct caccgccctt tatctcggct 721 ctctgatcaa agaggccggg ttccctccag gagtggtgaa cattgtgcca ggattcgggc 781 ccacagtggg agcagcaatt tcttctcacc ctcagatcaa caagatcgcc ttcaccggct 841 ccacagaggt tggaaaactg gttaaagaag ctgcgtcccg gagcaatctg aagcgggtga 901 cgctggagct gggggggaag aacccctgca tcgtgtgtgc ggacgctgac ttggacttgg 961 cagtggagtg tgcccatcag ggagtgttct tcaaccaagg ccagtgttgc acggcagcct 1021 ccagggtgtt cgtggaggag caggtctact ctgagtttgt caggcggagc gtggagtatg 1081 ccaagaaacg gcccgtggga gaccccttcg atgtcaaaac agaacagggg cctcagattg 1141 atcaaaagca gttcgacaaa aTCTTAGAGC TGATCGAGAG TGGGAAGAAG GAAGGGGCCA 1201 AGCTGGAATG CGGGGGCTCA GCCATGGAAG ACAAGGGGCT CTTCATCAAA CCCACTGTCT 1261 TCTCAGAAGT CACAGACAAC ATGCGGATTG CCAAAGAGGA GATTTTCGGG CCAGTGCAAC 1321 CAATACTGAA GTTCAAAAGT ATCGAAGAAG TGATAAAAAG AGCGAATAGC ACCGACTATG 1381 GACTCACAGC AGCTGTGTTC ACAAAAAATC TCGACAAAGC CCTGAAGTTG GCTTCTGCCT 1441 TAGAGTCTGG AACGGTCTGG ATCAACTGCT ACAACGCCCT CTATGCACAG GCTCCATTTG 1501 GTGGCTTTAA AATGTCAGGA AATGGCAGAG AACTAGGTGA ATACGCTTTG GCCGAATACA 1561 CAGAAGTGAA AACTGTCACC ATCAAACTTG GCGACAAGAA CCCCTGAGAC CCAGCTTTCT 1621 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1681 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1741 GTGGAAAGGA CGAGCAGCTT CCAAACTCTG AACACTAACG CGTTAAGTCg acaatcaacc 1801 tctggattac aaaatttgtg aaagatt