Construct: ORF TRCN0000489918
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020330.1_s317c1
- DNA Barcode:
- CGCTTCGAAGCACGTGGCCACATG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ULK4 (54986)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489918
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54986 | ULK4 | unc-51 like kinase 4 | NM_001322500.2 | 46.4% | 45% | (many diffs) |
| 2 | human | 54986 | ULK4 | unc-51 like kinase 4 | NM_017886.4 | 44.9% | 43.5% | (many diffs) |
| 3 | human | 54986 | ULK4 | unc-51 like kinase 4 | NR_136342.2 | 39.2% | (many diffs) | |
| 4 | human | 54986 | ULK4 | unc-51 like kinase 4 | NM_001322501.2 | 21.2% | 20% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1812
- ORF length:
- 1740
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaaaac tttattctgt atgaggagat cggaagagga agcaagactg 121 ttgtctataa agggcgacgg aagggaacaa tcaattttgt agccattctt tgtactgata 181 agtgcaaaag gcctgaaata accaactggg tccgtctcac ccgtgaaata aaacacaaga 241 atattgtaac ttttcatgaa tggtatgaaa caagcaacca cctctggcta gtggtggaac 301 tctgcacagg tggttcctta aaaacagtta ttgctcaaga tgaaaacctc ccagaagatg 361 ttgtgagaga atttggaatt gacctgatta gtggattaca tcatcttcat aaacttggca 421 ttctcttttg tgacatttct cctaggaaga tactcttgga agggcctggc acactgaagt 481 ttagcaactt ttgcttggca aaagtggaag gtgaaaattt ggaagagttc tttgctttgg 541 tggcagcaga ggaaggagga ggtgataatg gggaaaatgt cctgaagaaa agcatgaaaa 601 gtagagtcaa aggatctcct gtatatacag caccagaagt tgtgaggggt gctgactttt 661 ccatctccag tgacctctgg tctttgggct gtctgcttta tgaaatgttt tcaggaaaac 721 ctccattctt ctcagaaagt atttcagaat taactgaaaa gatcttatgt gaagatcctt 781 tgccacctat tccgaaagat tcttctcgtc ctaaagcttc ttcagatttt attaatttgc 841 ttgatgggtt acttcaaaga gatcctcaga aaagattgac ttggacaagg ctactgcagc 901 attcattttg gaagaaagct tttgctggag cagatcagga atcaagcgtc gaagatctca 961 gtctcagcag aaacactatg gagtgttctg ggccacaaga ttccaaggag cttttgcaga 1021 actctcagag tagacaagca aaagggcaca agagtggtca accactaggt cactctttca 1081 gactagaaaa tccaactgag tttcggccta agagtactct tgagggtcaa ttgaatgaat 1141 ccatgtttct tctcagttct cgtcctactc ccagaactag cactgcagtg gaagtaagtc 1201 ctggtgagga tatgactcac tgttcaccac agaagacttc tcctctgacc aagattacaa 1261 gtggacacct gagtcagcag gacctggaat cccagatgag agagcttatc tacacggact 1321 cagatcttgt tgtcaccccc attatcgaca atccaaagat aatgaaacag ccaccagtta 1381 aatttgatgc aaaaatattg catctaccaa catattcagt ggataagtta ttatttcTGA 1441 AAGATCAAGA TTGGAATGAC TTTTTGCAAC AAGTGTGCTC GCAGATCGAC TCCACTGAGA 1501 AGAGCATGGG GGCCTCCCGA GCCAAGCTGA ATCTCCTTTG CTATTTGTGC GTGGTGGCTG 1561 GTCACCAGGA GGTGGCCACC AGGCTCCTCC ATTCCCCCCT GTTCCAATTG CTAATCCAGC 1621 ATTTGCGGAT AGCTCCAAAC TGGGATATAC GGGCCAAGGT TGCTCACGTA ATTGGTTTAC 1681 TGGCTTCGCA CACAGCTGAG CTCCAGGAAA ATACACCTGT TGTTGAGACT ACAAGCTCCA 1741 TTGGAATCGG GATTTTGAAC TGTCTTGTTC AACACTCCAC TCCAGTGCCT AGACAGTGCC 1801 TTGTGTATGT ATGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1861 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1921 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGCTTCGAAG CACGTGGCCA 1981 CATGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt