Construct: ORF TRCN0000489926
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020617.1_s317c1
- DNA Barcode:
- TCGGACTTTGTCTTACAAAATCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- JAK1 (3716)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489926
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3716 | JAK1 | Janus kinase 1 | NM_001320923.1 | 100% | 100% | |
| 2 | human | 3716 | JAK1 | Janus kinase 1 | NM_001321852.2 | 100% | 100% | |
| 3 | human | 3716 | JAK1 | Janus kinase 1 | NM_001321853.2 | 100% | 100% | |
| 4 | human | 3716 | JAK1 | Janus kinase 1 | NM_001321854.2 | 100% | 100% | |
| 5 | human | 3716 | JAK1 | Janus kinase 1 | NM_001321855.2 | 100% | 100% | |
| 6 | human | 3716 | JAK1 | Janus kinase 1 | NM_001321856.1 | 100% | 100% | |
| 7 | human | 3716 | JAK1 | Janus kinase 1 | NM_002227.4 | 100% | 100% | |
| 8 | human | 3716 | JAK1 | Janus kinase 1 | NM_001321857.2 | 99.9% | 99.9% | 1456_1457insAGC |
| 9 | mouse | 16451 | Jak1 | Janus kinase 1 | NM_146145.2 | 89.3% | 94.8% | (many diffs) |
| 10 | mouse | 16451 | Jak1 | Janus kinase 1 | XM_006502812.3 | 89.3% | 94.8% | (many diffs) |
| 11 | mouse | 16451 | Jak1 | Janus kinase 1 | XM_011240451.2 | 89.3% | 94.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3534
- ORF length:
- 3462
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcagtat ctaaatataa aagaggactg caatgccatg gctttctgtg 121 ctaaaatgag gagctccaag aagactgagg tgaacctgga ggcccctgag ccaggggtgg 181 aagtgatctt ctatctgtcg gacagggagc ccctccggct gggcagtgga gagtacacag 241 cagaggaact gtgcatcagg gctgcacagg catgccgtat ctctcctctt tgtcacaacc 301 tctttgccct gtatgacgag aacaccaagc tctggtatgc tccaaatcgc accatcaccg 361 ttgatgacaa gatgtccctc cggctccact accggatgag gttctatttc accaattggc 421 atggaaccaa cgacaatgag cagtcagtgt ggcgtcattc tccaaagaag cagaaaaatg 481 gctacgagaa aaaaaagatt ccagatgcaa cccctctcct tgatgccagc tcactggagt 541 atctgtttgc tcagggacag tatgatttgg tgaaatgcct ggctcctatt cgagacccca 601 agaccgagca ggatggacat gatattgaga acgagtgtct agggatggct gtcctggcca 661 tctcacacta tgccatgatg aagaagatgc agttgccaga actgcccaag gacatcagct 721 acaagcgata tattccagaa acattgaata agtccatcag acagaggaac cttctcacca 781 ggatgcggat aaataatgtt ttcaaggatt tcctaaagga atttaacaac aagaccattt 841 gtgacagcag cgtgtccacg catgacctga aggtgaaata cttggctacc ttggaaactt 901 tgacaaaaca ttacggtgct gaaatatttg agacttccat gttactgatt tcatcagaaa 961 atgagatgaa ttggtttcat tcgaatgacg gtggaaacgt tctctactac gaagtgatgg 1021 tgactgggaa tcttggaatc cagtggaggc ataaaccaaa tgttgtttct gttgaaaagg 1081 aaaaaaataa actgaagcgg aaaaaactgg aaaataaaca caagaaggat gaggagaaaa 1141 acaagatccg ggaagagtgg aacaattttt cttacttccc tgaaatcact cacattgtaa 1201 taaaggagtc tgtggtcagc attaacaagc aggacaacaa gaaaatggaa ctgaagctct 1261 cttcccacga ggaggccttg tcctttgtgt ccctggtaga tggctacttc cggctcacag 1321 cagatgccca tcattacctc tgcaccgacg tggccccccc gttgatcgtc cacaacatac 1381 agaatggctg tcatggtcca atctgtacag aatacgccat caataaattg cggcaagaag 1441 gaagcgagga ggggatgtac gtgctgaggt ggagctgcac cgactttgac aacatcctca 1501 tgaccgtcac ctgctttgag aagtctgagc aggtgcaggg tgcccagaag cagttcaaga 1561 actttcagat cgaggtgcag aagggccgct acagtctgca cggttcggac cgcagcttcc 1621 ccagcttggg agacctcatg agccacctca agaagcagat cctgcgcacg gataacatca 1681 gcttcatgct aaaacgctgc tgccagccca agccccgaga aatctccaac ctgctggtgg 1741 ctactaagaa agcccaggag tggcagcccg tctaccccat gagccagctg agtttcgatc 1801 ggatcctcaa gaaggatctg gtgcagggcg agcaccttgg gagaggcacg agaacacaca 1861 tctattctgg gaccctgatg gattacaagg atgacgaagg aacttctgaa gagaagaaga 1921 taaaagtgat cctcaaagtc ttagacccca gccacaggga tatttccctg gccttcttcg 1981 aggcagccag catgatgaga caggtctccc acaaacacat cgtgtacctc tatggcgtct 2041 gtgtccgcga cgtggagaat atcatggtgg aagagtttgt ggaagggggt cctctggatc 2101 tcttcatgca ccggaaaagc gatgtcctta ccacaccatg gaaattcaaa gttgccaaac 2161 agctggccag tgccctgagc tacttggagg ataaagacct ggtccatgga aatgtgtgta 2221 ctaaaaacct cctcctggcc cgtgagggca tcgacagtga gtgtggccca ttcatcaagc 2281 tcagtgaccc cggcatcccc attacggtgc tgtctaggca agaatgcatt gaacgaatcc 2341 catggattgc tcctgagtgt gttgaggact ccaagaacct gagtgtggct gctgacaagt 2401 ggagctttgg aaccacgctc tgggaaatct gctacaatgg cgagatcccc ttgaaagaca 2461 agacgctgat tgagaaagag agattctatg aaagccggtg caggccagtg acaccatcat 2521 gtaaggagct ggctgacctc atgacccgct gcatgaacta tgaccccaat cagaggcctt 2581 tcttccgagc catcatgaga gacattaata agcttgaaga gcagaatcca gatattgttt 2641 cagaaaaaaa accagcaact gaagtggacc ccacacattt tgaaaagcgc ttcctaaaga 2701 ggatccgtga cttgggagag ggccactttg ggaaggttga gctctgcagg tatgaccccg 2761 aaggggacaa tacaggggag caggtggctg ttaaatctct gaagcctgag agtggaggta 2821 accacatagc tgatctgaaa aaggaaatcg agatcttaag gaacctctat catgagaaca 2881 ttgtgaagta caaaggaatc tgcacagaag acggaggaaa tggtattaag ctcatcatgg 2941 aatttctgcc ttcgggaagc cttaaggaat atcttccaaa gaataagaac aaaataaacc 3001 tcaaacagca gctaaaatat gccgttcaga tttgtaaggg gatggactat ttgggttctc 3061 ggcaatacgt tcaccgggac ttggcagcaa gaaatgtcct tgttGAGAGT GAACACCAAG 3121 TGAAAATTGG AGACTTCGGT TTAACCAAAG CAATTGAAAC CGATAAGGAG TATTACACCG 3181 TCAAGGATGA CCGGGACAGC CCTGTGTTTT GGTATGCTCC AGAATGTTTA ATGCAATCTA 3241 AATTTTATAT TGCCTCTGAC GTCTGGTCTT TTGGAGTCAC TCTGCATGAG CTGCTGACTT 3301 ACTGTGATTC AGATTCTAGT CCCATGGCTT TGTTCCTGAA AATGATAGGC CCAACCCATG 3361 GCCAGATGAC AGTCACAAGA CTTGTGAATA CGTTAAAAGA AGGAAAACGC CTGCCGTGCC 3421 CACCTAACTG TCCAGATGAG GTTTATCAAC TTATGAGGAA ATGCTGGGAA TTCCAACCAT 3481 CCAATCGGAC AAGCTTTCAG AACCTTATTG AAGGATTTGA AGCACTTTTA AAATAAGACC 3541 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 3601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 3661 ATATATCTTG TGGAAAGGAC GATCGGACTT TGTCTTACAA AATCACACGC GTTAAGTCga 3721 caatcaacct ctggattaca aaatttgtga aagatt