Construct: ORF TRCN0000489936
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020457.1_s317c1
- DNA Barcode:
- ATCGTACGAATTATGCCTGTGTGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ERBB3 (2065)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489936
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2065 | ERBB3 | erb-b2 receptor tyrosine ki... | NM_001982.3 | 99.9% | 100% | 3348G>A |
| 2 | human | 2065 | ERBB3 | erb-b2 receptor tyrosine ki... | NM_001005915.1 | 12.9% | 10.9% | (many diffs) |
| 3 | mouse | 13867 | Erbb3 | erb-b2 receptor tyrosine ki... | NM_010153.1 | 86.6% | 90.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 4098
- ORF length:
- 4026
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagggcg aacgacgctc tgcaggtgct gggcttgctt ttcagcctgg 121 cccggggctc cgaggtgggc aactctcagg cagtgtgtcc tgggactctg aatggcctga 181 gtgtgaccgg cgatgctgag aaccaatacc agacactgta caagctctac gagaggtgtg 241 aggtggtgat ggggaacctt gagattgtgc tcacgggaca caatgccgac ctctccttcc 301 tgcagtggat tcgagaagtg acaggctatg tcctcgtggc catgaatgaa ttctctactc 361 taccattgcc caacctccgc gtggtgcgag ggacccaggt ctacgatggg aagtttgcca 421 tcttcgtcat gttgaactat aacaccaact ccagccacgc tctgcgccag ctccgcttga 481 ctcagctcac cgagattctg tcagggggtg tttatattga gaagaacgat aagctttgtc 541 acatggacac aattgactgg agggacatcg tgagggaccg agatgctgag atagtggtga 601 aggacaatgg cagaagctgt cccccctgtc atgaggtttg caaggggcga tgctggggtc 661 ctggatcaga agactgccag acattgacca agaccatctg tgctcctcag tgtaatggtc 721 actgctttgg gcccaacccc aaccagtgct gccatgatga gtgtgccggg ggctgctcag 781 gccctcagga cacagactgc tttgcctgcc ggcacttcaa tgacagtgga gcctgtgtac 841 ctcgctgtcc acagcctctt gtctacaaca agctaacttt ccagctggaa cccaatcccc 901 acaccaagta tcagtatgga ggagtttgtg tagccagctg tccccataac tttgtggtgg 961 atcaaacatc ctgtgtcagg gcctgtcctc ctgacaagat ggaagtagat aaaaatgggc 1021 tcaagatgtg tgagccttgt gggggactat gtcccaaagc ctgtgaggga acaggctctg 1081 ggagccgctt ccagactgtg gactcgagca acattgatgg atttgtgaac tgcaccaaga 1141 tcctgggcaa cctggacttt ctgatcaccg gcctcaatgg agacccctgg cacaagatcc 1201 ctgccctgga cccagagaag ctcaatgtct tccggacagt acgggagatc acaggttacc 1261 tgaacatcca gtcctggccg ccccacatgc acaacttcag tgttttttcc aatttgacaa 1321 ccattggagg cagaagcctc tacaaccggg gcttctcatt gttgatcatg aagaacttga 1381 atgtcacatc tctgggcttc cgatccctga aggaaattag tgctgggcgt atctatataa 1441 gtgccaatag gcagctctgc taccaccact ctttgaactg gaccaaggtg cttcgggggc 1501 ctacggaaga gcgactagac atcaagcata atcggccgcg cagagactgc gtggcagagg 1561 gcaaagtgtg tgacccactg tgctcctctg ggggatgctg gggcccaggc cctggtcagt 1621 gcttgtcctg tcgaaattat agccgaggag gtgtctgtgt gacccactgc aactttctga 1681 atggggagcc tcgagaattt gcccatgagg ccgaatgctt ctcctgccac ccggaatgcc 1741 aacccatgga gggcactgcc acatgcaatg gctcgggctc tgatacttgt gctcaatgtg 1801 cccattttcg agatgggccc cactgtgtga gcagctgccc ccatggagtc ctaggtgcca 1861 agggcccaat ctacaagtac ccagatgttc agaatgaatg tcggccctgc catgagaact 1921 gcacccaggg gtgtaaagga ccagagcttc aagactgttt aggacaaaca ctggtgctga 1981 tcggcaaaac ccatctgaca atggctttga cagtgatagc aggattggta gtgattttca 2041 tgatgctggg cggcactttt ctctactggc gtgggcgccg gattcagaat aaaagggcta 2101 tgaggcgata cttggaacgg ggtgagagca tagagcctct ggaccccagt gagaaggcta 2161 acaaagtctt ggccagaatc ttcaaagaga cagagctaag gaagcttaaa gtgcttggct 2221 cgggtgtctt tggaactgtg cacaaaggag tgtggatccc tgagggtgaa tcaatcaaga 2281 ttccagtctg cattaaagtc attgaggaca agagtggacg gcagagtttt caagctgtga 2341 cagatcatat gctggccatt ggcagcctgg accatgccca cattgtaagg ctgctgggac 2401 tatgcccagg gtcatctctg cagcttgtca ctcaatattt gcctctgggt tctctgctgg 2461 atcatgtgag acaacaccgg ggggcactgg ggccacagct gctgctcaac tggggagtac 2521 aaattgccaa gggaatgtac taccttgagg aacatggtat ggtgcataga aacctggctg 2581 cccgaaacgt gctactcaag tcacccagtc aggttcaggt ggcagatttt ggtgtggctg 2641 acctgctgcc tcctgatgat aagcagctgc tatacagtga ggccaagact ccaattaagt 2701 ggatggccct tgagagtatc cactttggga aatacacaca ccagagtgat gtctggagct 2761 atggtgtgac agtttgggag ttgatgacct tcggggcaga gccctatgca gggctacgat 2821 tggctgaagt accagacctg ctagagaagg gggagcggtt ggcacagccc cagatctgca 2881 caattgatgt ctacatggtg atggtcaagt gttggatgat tgatgagaac attcgcccaa 2941 cctttaaaga actagccaat gagttcacca ggatggcccg agacccacca cggtatctgg 3001 tcataaagag agagagtggg cctggaatag cccctgggcc agagccccat ggtctgacaa 3061 acaagaagct agaggaagta gagctggagc cagaactaga cctagaccta gacttggaag 3121 cagaggagga caacctggca accaccacac tgggctccgc cctcagccta ccagttggaa 3181 cacttaatcg gccacgtggg agccagagcc ttttaagtcc atcatctgga tacatgccca 3241 tgaaccaggg taatcttggg gagtcttgcc aggagtctgc agtttctggg agcagtgaac 3301 ggtgcccccg tccagtctct ctacacccaa tgccacgggg atgcctggca tcagagtcat 3361 cagaggggca tgtaacaggc tctgaggctg agctccagga gaaagtgtca atgtgtagaa 3421 gccggagcag gagccggagc ccacggccac gcggagatag cgcctaccat tcccagcgcc 3481 acagtctgct gactcctgtt accccactct ccccacccgg gttagaggaa gaggatgtca 3541 acggttatgt catgccagat acacacctca aaggtactcc ctcctcccgg gaaggcaccc 3601 tttcttcagt gggtctcagt tctgtcctgg gtactgaaga agaagatgaa gatgaggagt 3661 atgaatacat gaaCCGGAGG AGAAGGCACA GTCCACCTCA TCCCCCTAGG CCAAGTTCCC 3721 TTGAGGAGCT GGGTTATGAG TACATGGATG TGGGGTCAGA CCTCAGTGCC TCTCTGGGCA 3781 GCACACAGAG TTGCCCACTC CACCCTGTAC CCATCATGCC CACTGCAGGC ACAACTCCAG 3841 ATGAAGACTA TGAATATATG AATCGGCAAC GAGATGGAGG TGGTCCTGGG GGTGATTATG 3901 CAGCCATGGG GGCCTGCCCA GCATCTGAGC AAGGGTATGA AGAGATGAGA GCTTTTCAGG 3961 GGCCTGGACA TCAGGCCCCC CATGTCCATT ATGCCCGCCT AAAAACTCTA CGTAGCTTAG 4021 AGGCTACAGA CTCTGCCTTT GATAACCCTG ATTACTGGCA TAGCAGGCTT TTCCCCAAGG 4081 CTAATGCCCA GAGAACGTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 4141 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 4201 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATCG TACGAATTAT 4261 GCCTGTGTGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt