Construct: ORF TRCN0000489940
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020794.1_s317c1
- DNA Barcode:
- CGGGTAATCTATGTTAATCCTTGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- P2RY1 (5028)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489940
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5028 | P2RY1 | purinergic receptor P2Y1 | NM_002563.5 | 99.9% | 100% | 786A>G |
| 2 | mouse | 18441 | P2ry1 | purinergic receptor P2Y, G-... | NM_001282016.1 | 87.2% | 94.9% | (many diffs) |
| 3 | mouse | 18441 | P2ry1 | purinergic receptor P2Y, G-... | NM_008772.5 | 87.2% | 94.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1191
- ORF length:
- 1119
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaccgag gtgctgtggc cggctgtccc caacgggacg gacgctgcct 121 tcctggccgg tccgggttcg tcctggggga acagcacggt cgcctccact gccgccgtct 181 cctcgtcgtt caaatgcgcc ttgaccaaga cgggcttcca gttttactac ctgccggctg 241 tctacatctt ggtattcatc atcggcttcc tgggcaacag cgtggccatc tggatgttcg 301 tcttccacat gaagccctgg agcggcatct ccgtgtacat gttcaatttg gctctggccg 361 acttcttgta cgtgctgact ctgccagccc tgatcttcta ctacttcaat aaaacagact 421 ggatcttcgg ggatgccatg tgtaaactgc agaggttcat ctttcatgtg aacctctatg 481 gcagcatctt gtttctgaca tgcatcagtg cccaccggta cagcggtgtg gtgtaccccc 541 tcaagtccct gggccggctc aaaaagaaga atgcgatctg tatcagcgtg ctggtgtggc 601 tcattgtggt ggtggcgatc tcccccatcc tcttctactc aggtaccggg gtccgcaaaa 661 acaaaaccat cacctgttac gacaccacct cagacgagta cctgcgaagt tatttcatct 721 acagcatgtg CACGACCGTG GCCATGTTCT GTGTCCCCTT GGTGCTGATT CTGGGCTGTT 781 ACGGATTAAT TGTGAGAGCT TTGATTTACA AAGATCTGGA CAACTCTCCT CTGAGGAGAA 841 AATCGATTTA CCTGGTGATC ATTGTACTGA CTGTTTTTGC TGTGTCTTAC ATCCCTTTCC 901 ATGTGATGAA AACGATGAAC TTGAGGGCCC GGCTTGATTT TCAGACCCCA GCAATGTGTG 961 CTTTCAATGA CAGGGTTTAT GCCACGTATC AGGTGACAAG AGGTCTAGCA AGTCTCAACA 1021 GTTGTGTGGA CCCCATTCTC TATTTCTTGG CGGGAGATAC TTTCAGAAGG AGACTCTCCC 1081 GAGCCACAAG GAAAGCTTCT AGAAGAAGTG AGGCAAATTT GCAATCCAAG AGTGAAGACA 1141 TGACCCTCAA TATTTTACCT GAGTTCAAGC AGAATGGAGA TACAAGCCTG TAGCAACCAG 1201 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1261 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1321 TATCTTGTGG AAAGGACGAC GGGTAATCTA TGTTAATCCT TGCACGCGTT AAGTCgacaa 1381 tcaacctctg gattacaaaa tttgtgaaag att