Construct: ORF TRCN0000489954
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021174.1_s317c1
- DNA Barcode:
- CTCTACCCACCGTTAGCTGGATCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RXFP2 (122042)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489954
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 122042 | RXFP2 | relaxin family peptide rece... | NM_130806.5 | 100% | 100% | |
| 2 | human | 122042 | RXFP2 | relaxin family peptide rece... | NM_001166058.1 | 96.8% | 96.8% | 855_856ins72 |
| 3 | human | 122042 | RXFP2 | relaxin family peptide rece... | XM_017020389.1 | 50.6% | 50.5% | 1146G>T;1148_1149delGCins1115 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2334
- ORF length:
- 2262
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgattgtt tttctggttt ttaaacatct cttcagcctc agattgatta 121 caatgttctt tctacttcat ttcatcgttc tgatcaatgt caaagatttt gcactgactc 181 aaggtagcat gatcactcct tcatgccaaa aaggatattt tccctgtggg aatcttacca 241 agtgcttacc ccgagctttt cactgtgatg gcaaggatga ctgtgggaac ggggcggacg 301 aagagaactg tggtgacact agtggatggg cgaccatatt tggcacagtg catggaaatg 361 ctaacagcgt ggccttaaca caggagtgct ttctaaaaca gtatccacaa tgctgtgact 421 gcaaagaaac tgaattggaa tgtgtaaatg gtgacttaaa gtctgtgccg atgatttcta 481 acaatgtgac attactgtct cttaagaaaa acaaaatcca cagtcttcca gataaagttt 541 tcatcaaata cacaaaactt aaaaagatat ttcttcagca taattgcatt agacacatat 601 ccaggaaagc attttttgga ttatgtaatc tgcaaatatt atatctcaac cacaactgca 661 tcacaaccct cagacctgga atattcaaag acttacatca gctaacttgg ctaattctag 721 atgacaatcc aataaccaga atttcacagc gcttgtttac gggattaaat tccttgtttt 781 tcctgtctat ggttaataac tacttagaag ctcttcccaa gcagatgtgt gcccaaatgc 841 ctcaactcaa ctgggtggat ttggaaggca atagaataaa gtatctcaca aattctacgt 901 ttctgtcgtg cgattcgctc acagtgctgt ttctgcctag aaatcaaatt ggttttgttc 961 cagagaagac attttcttca ttaaaaaatt taggagaact ggatctgtct agcaatacga 1021 taacggaact atcacctcac ctttttaaag acttgaagct tctacaaaag ctgaacctgt 1081 catccaatcc tcttatgtat cttcacaaga accagtttga aagtcttaaa caacttcagt 1141 ctctagacct ggaaaggata gagattccaa atataaacac acgaatgttt caacccatga 1201 agaatctttc tcacatttat ttcaaaaact ttcgatactg ctcctatgct ccccatgtcc 1261 gaatatgtat gcccttgacg gacggcattt cttcatttga ggacctcttg gctaacaata 1321 tcctcagaat atttgtctgg gttatagctt tcattacctg ctttggaaat ctttttgtca 1381 ttggcatgag atctttcatt aaagctgaaa atacaactca cgctatgtcc atcaaaatcc 1441 tttgttgtgc tgattgcctg atgggtgttt acttgttctt tgttggcatt ttcgatataa 1501 aataccgagg gcagtatcag aagtatgcct tgctgtggat ggagagcgtg cagtgccgcc 1561 tcatggggtt cctggccatg ctgtccaccg aagtctctgt tctgctactg acctacttga 1621 ctttggagaa gttcctggtc attgtcttcc ccttcagtaa cattcgacct ggaaaacggc 1681 agacctcagt catcctcatt tgcatctgga tggcgggatt tttaatagct gtaattccat 1741 tttggaataa ggattatttt ggaaactttt atgggaaaaa tggagtatgt ttcccacttt 1801 attatgacca aacagaagat attggaagca aagggtattc tcttggaatt ttcctaggtg 1861 tgaacttgct ggcttttctc atcattgtgt tttcctatat tactatgttc tgttccattc 1921 aaaaaaccgc cttgcagacc acagaagtaa GGAATTGTTT TGGAAGAGAG GTGGCTGTTG 1981 CAAATCGTTT CTTTTTTATA GTGTTCTCTG ATGCCATCTG CTGGATTCCT GTATTTGTAG 2041 TTAAAATCCT TTCCCTCTTC CGGGTGGAAA TACCAGACAC AATGACTTCC TGGATAGTGA 2101 TTTTTTTCCT TCCAGTTAAC AGTGCTTTGA ATCCAATCCT CTATACTCTC ACAACCAACT 2161 TTTTTAAGGA CAAGTTGAAA CAGCTGCTGC ACAAACATCA GAGGAAATCA ATTTTCAAAA 2221 TTAAAAAAAA AAGTTTATCT ACATCCATTG TGTGGATAGA GGACTCCTCT TCCCTGAAAC 2281 TTGGGGTTTT GAACAAAATA ACACTTGGAG ACAGTATAAT GAAACCAGTT TCCTAAGACC 2341 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2401 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2461 ATATATCTTG TGGAAAGGAC GACTCTACCC ACCGTTAGCT GGATCCACGC GTTAAGTCga 2521 caatcaacct ctggattaca aaatttgtga aagatt