Construct: ORF TRCN0000489957
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021314.1_s317c1
- DNA Barcode:
- ACTCGTGTGTTCACGGGGATTATG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RFC1 (5981)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489957
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5981 | RFC1 | replication factor C subunit 1 | NM_002913.5 | 99.9% | 100% | 2541A>G;3132T>C |
| 2 | human | 5981 | RFC1 | replication factor C subunit 1 | NM_001204747.2 | 99.8% | 99.9% | 1885_1887delGCA;2544A>G;3135T>C |
| 3 | human | 5981 | RFC1 | replication factor C subunit 1 | NM_001363495.2 | 97.6% | 97.7% | 640_641ins78;2463A>G;3054T>C |
| 4 | human | 5981 | RFC1 | replication factor C subunit 1 | NM_001363496.2 | 97.5% | 97.6% | (many diffs) |
| 5 | human | 5981 | RFC1 | replication factor C subunit 1 | XM_011513731.1 | 85.2% | 85.2% | 1486_1487ins507;2034A>G;2625T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 59
- ORF end:
- 3500
- ORF length:
- 3441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaccat 61 ggacattcgg aaattctttg gagtaatacc aagtggaaag aaacttgtaa gtgaaacagt 121 aaagaagaat gagaaaacaa agtctgatga agaaacttta aaagcaaaga aaggaataaa 181 ggaaatcaag gtaaatagct cccgtaaaga ggatgacttc aaacaaaagc aaccaagcaa 241 gaaaaagagg atcatctatg attcagattc agagtcagag gagacgttgc aggtaaaaaa 301 tgccaaaaag ccaccagaaa aactgccagt atcttctaaa cctggtaaaa tttcacggca 361 ggatcctgtt acatacattt cagaaacaga tgaagaagat gactttatgt gtaagaaggc 421 ggcctctaaa tcaaaagaga atggaagatc tacaaatagt catcttggaa catcaaacat 481 gaaaaagaat gaagaaaaca ctaagaccaa gaataagcct ttatcaccaa taaaacttac 541 acccacatca gtacttgatt attttggaac tggaagtgtc caaagatcta ataagaagat 601 ggtggcaagc aaaagaaaag agctttcaca aaatacagat gagtctggat taaatgatga 661 agccatcgcc aagcaattac agcttgatga agatgcggag ctggagaggc agttgcatga 721 agatgaagag tttgccagaa cattagccat gttggatgaa gaacccaaga ccaaaaaggc 781 tcgaaaggac acagaagcgg gagaaacgtt ttcatctgtc caagccaatt taagtaaagc 841 agaaaaacat aaatatcctc ataaagtaaa aacagcacaa gtttcagatg aaagaaagag 901 ctacagtcct aggaagcaaa gtaaatatga aagttcaaaa gaatctcagc aacattccaa 961 gtcatcagct gacaaaatag gagaagtctc ttctcccaag gccagttcta agctggcaat 1021 tatgaaaaga aaagaagaga gctcttataa agaaatagag cctgtggcct caaaaagaaa 1081 agaaaatgcc attaaattga aaggagagac aaaaactcct aagaaaacca aaagttctcc 1141 agctaaaaaa gagtctgtaa gtcctgaaga ttctgaaaag aaacgcacta attatcaagc 1201 ttatcgaagc tacttaaatc gagaaggtcc caaggctctg ggctccaaag aaataccaaa 1261 gggagctgaa aattgcttgg aaggccttat atttgtaatc acaggcgtgc tggagtctat 1321 tgaacgagat gaggccaagt ctctaattga acgttatggg ggaaaagtaa caggaaatgt 1381 cagcaagaaa acaaattatc ttgtcatggg tcgtgatagt ggacagtcca agagtgataa 1441 ggccgcagcc ttggggacaa aaattattga tgaagatggc ctgttgaatc tgattcggac 1501 tatgccaggc aagaaatcca agtatgaaat agcagttgaa actgagatga agaaagagtc 1561 caaactggag agaacacccc aaaaaaatgt ccaaggaaaa agaaaaatta gtccatctaa 1621 aaaggaatca gaatctaaaa agagcaggcc gacttccaaa agggacagtt tggcaaagac 1681 aataaaaaag gaaacagatg tgttttggaa aagcctggat ttcaaggagc aggtggctga 1741 ggagacaagt ggtgacagca aggctaggaa tttggctgat gacagcagtg aaaacaaagt 1801 ggaaaatttg ctctgggtgg ataaatataa gccaacctcg ctcaagacca taattggaca 1861 gcaaggtgac cagagctgtg ccaacaaact cctacgctgg ctccgaaact ggcaaaagag 1921 ttcttccgaa gataaaaaac acgcaaagtt tggtaaattt tccggcaaag atgatggctc 1981 tagttttaaa gcagcgttgc tgtcaggccc tcctggtgtt ggcaaaacca ccacagcttc 2041 cctggtgtgt caggagttgg gatacagcta cgtggaactg aatgcaagtg acacccggag 2101 taagagcagt ttgaaggcga ttgttgctga gtcactgaac aataccagca tcaaaggctt 2161 ttattcaaat ggagcagcct cttcagtaag cacgaaacat gctctcatca tggatgaagt 2221 agatggcatg gcaggcaatg aggatagggg aggaattcag gaattaattg gcctgataaa 2281 acatactaaa attcccatta tttgtatgtg caatgataga aatcatccca agattcgctc 2341 tctggttcat tattgttttg atcttcgttt tcaaagacct cgggttgaac agattaaggg 2401 tgctatgatg tctattgcat ttaaagaagg tttaaagatt ccccctccag ctatgaatga 2461 aataattttg ggagccaatc aagatatcag acaggtttta cataatctga gtatgtggtg 2521 tgcacgaagt aaagcattaa cctatgacca ggccaaagct gattctcaca gagccaaaaa 2581 ggatatcaaa atgggcccgt ttgatgttgc ccggaaagtg tttgcagctg gagaggagac 2641 tgctcacatg tcacttgtgg acaagtcaga tctctttttt catgattatt caatagcacc 2701 cctcttcgtc caggaaaatt acatacacgt gaagcctgta gcagcagggg gtgacatgaa 2761 aaagcacctg atgcttttaa gcagagcagc agacagcata tgcgatggtg acctagtgga 2821 cagccagatc cggagtaagc aaaactggag tcttctgcct gcgcaggcca tttatgccag 2881 tgttcttcct ggagagttga tgagggggta catgacccag tttcccacct tcccaagctg 2941 gctggggaag cactcgtcta caggcaaaca tgatcgtatt gttcaggacc tggccttgca 3001 tatgagtctc agaacttact ccagcaaaag gactgtaaac atggattatc tgtcgcttct 3061 aagggatgca cttgtacagc ccttgacctc acaaggagta gacggagtac aggatgttgt 3121 tgcacttatg gacacatatt atttgatgaa agaagacttt gagaatatca tggaaatcag 3181 cagctggggc ggcaaaccta gtcccttttc aaagctggat cccaaggtga aagcagcctt 3241 cacaagagct tacaataagg aagcccacct tactccatac tcacttcaag ctataaaggc 3301 atctagacac agcacaagcc catccctgga ttcggaatac aatgaagaat taaatgAAGA 3361 TGACTCTCAA TCTGATGAGA AAGACCAAGA TGCTATAGAA ACTGATGCCA TGATCAAGAA 3421 AAAGACAAAA TCTTCAAAGC CTTCAAAACC AGAAAAAGAT AAGGAGCCCA GAAAAGGAAA 3481 AGGAAAAAGT TCGAAGAAAT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 3541 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 3601 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC TCGTGTGTTC 3661 ACGGGGATTA TGACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 3721 tt