Construct: ORF TRCN0000491263
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020764.2_s317c1
- DNA Barcode:
- TGATGCCCTCAATCCTATAAGTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GPR152 (390212)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491263
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 390212 | GPR152 | G protein-coupled receptor 152 | NM_206997.1 | 99% | 99.1% | 1_12del;237G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1470
- ORF length:
- 1398
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaagct gacctgggtg ccactggcca caggccccgc acagagcttg 121 atgatgagga ctcctacccc caaggtggct gggacacggt cttcctggtg gccctgctgc 181 tccttgggct gccagccaat gggttgatgg cgtggctggc cggctcccag gcccggcatg 241 gagctggcac gcgtctggcg ctgctcctgc tcagcctggc cctctctgac ttcttattcc 301 tggcagcagc ggccttccag atcctagaga tccggcatgg gggacactgg ccgctgggga 361 cagctgcctg ccgcttctac tacttcctat ggggcgtgtc ctactcctcc ggcctcttcc 421 tgctggccgc cctcagcctc gaccgctgcc tgctggcgct gtgcccacac tggtaccctg 481 ggcaccgccc agtccgcctg cccctctggg tctgcgccgg tgtctgggtg ctggccacac 541 tcttcagcgt gccctggctg gtcttccccg aggctgccgt ctggtggtac gacctggtca 601 tctgcctgga cttctgggac agcgaggagc tgtcgctgag gatgctggag gtcctggggg 661 gcttcctgcc tttcctcctg ctgctcgtct gccacgtgct cacccaggcc acagcctgtc 721 gcacctgcca ccgccaacag cagcccgcag cctgccgggg cttcgcccgt gtggccagga 781 ccattctgtc agcctatgtg gtcctgaggc tgccctacca gctggcccag ctgctctacc 841 tggccttcct gtgggacgtc tactctggct acctgctctg ggaggccctg gtctactccg 901 actacctgat cctactcaac agctgcctca gccccttcct ctgcctcatg gccagtgccg 961 acctccggac cctgctgcgc tccgtgctct cgtccttcgc ggcagctctc tgcgaggagc 1021 ggccgggcag cttcacgccc actgagccac agacccagct agattctgag ggtccaactc 1081 tgccagagcc gatggcagag gcccagtcac agatggatcc tgtggcccag cctcaggtga 1141 accccacact ccagccacga tcggatccca cagctcagcc acagctgaac ccTACGGCCC 1201 AGCCACAGTC GGATCCCACA GCCCAGCCAC AGCTGAACCT CATGGCCCAG CCACAGTCAG 1261 ATTCTGTGGC CCAGCCACAG GCAGACACTA ACGTCCAGAC CCCTGCACCT GCTGCCAGTT 1321 CTGTGCCCAG TCCCTGTGAT GAAGCTTCCC CAACCCCATC CTCGCATCCT ACCCCAGGGG 1381 CCCTTGAGGA CCCAGCCACA CCTCCTGCCT CTGAAGGAGA AAGCCCCAGC AGCACCCCGC 1441 CAGAGGCGGC CCCGGGCGCA GGCCCCACGT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG 1501 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1561 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 1621 ATGCCCTCAA TCCTATAAGT TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1681 ttgtgaaaga tt