Construct: ORF TRCN0000491290
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016664.1_s317c1
- Derived from:
- ccsbBroadEn_08375
- DNA Barcode:
- CATCCCTAGGTAATGCTTTCTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SSH1 (54434)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491290
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54434 | SSH1 | slingshot protein phosphata... | NM_018984.4 | 99.9% | 99.9% | 5C>T;2805C>T;2865A>G |
| 2 | human | 54434 | SSH1 | slingshot protein phosphata... | XM_005268984.4 | 98.3% | 97.9% | (many diffs) |
| 3 | human | 54434 | SSH1 | slingshot protein phosphata... | XM_017019491.2 | 97% | 96.6% | (many diffs) |
| 4 | human | 54434 | SSH1 | slingshot protein phosphata... | XM_011538497.1 | 96.2% | 94.6% | (many diffs) |
| 5 | human | 54434 | SSH1 | slingshot protein phosphata... | XM_005268985.2 | 92.3% | 92.3% | 0_1ins240;2565C>T;2625A>G |
| 6 | human | 54434 | SSH1 | slingshot protein phosphata... | XM_011538500.3 | 92.3% | 92.3% | 0_1ins240;2565C>T;2625A>G |
| 7 | human | 54434 | SSH1 | slingshot protein phosphata... | XM_011538501.3 | 92.3% | 92.3% | 0_1ins240;2565C>T;2625A>G |
| 8 | human | 54434 | SSH1 | slingshot protein phosphata... | XM_011538499.1 | 89.9% | 88.3% | (many diffs) |
| 9 | human | 54434 | SSH1 | slingshot protein phosphata... | XM_017019492.1 | 87.6% | 87.2% | (many diffs) |
| 10 | human | 54434 | SSH1 | slingshot protein phosphata... | XM_017019493.1 | 70.1% | 68.4% | (many diffs) |
| 11 | human | 54434 | SSH1 | slingshot protein phosphata... | XM_017019494.1 | 70.1% | 68.4% | (many diffs) |
| 12 | human | 54434 | SSH1 | slingshot protein phosphata... | NM_001161330.2 | 64.3% | 61.1% | (many diffs) |
| 13 | human | 54434 | SSH1 | slingshot protein phosphata... | NM_001161331.1 | 61.3% | 56.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3213
- ORF length:
- 3147
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt cctggtgacc ctgcagcgct cgcccacgcc cagcgccgcc tcctcctcgg 121 ccagcaacag cgagttggag gctggcagcg aagaagatcg aaaattaaac ctcagcttaa 181 gtgagagctt tttcatggtg aaaggcgcag ccctcttctt acaacaggga agcagccctc 241 aaggccagcg gagtcttcag cacccccaca agcatgcagg tgatctgcct caacatcttc 301 aggtgatgat caaccttctg cgttgcgaag acagaatcaa gctggcagtg cgcctggaga 361 gcgcctgggc ggaccgggtc cggtacatgg tggtggtgta cagcagcggg cgccaggaca 421 ccgaggagaa tatcttgctg ggagtggact tttccagtaa ggaaagtaaa agctgcacca 481 ttgggatggt tctccgactg tggagcgaca cgaaaatcca ccttgatgga gatggtgggt 541 tcagcgtgag cacagcagga aggatgcaca tatttaagcc tgtgtctgtc caggccatgt 601 ggtctgccct gcaggtgctt cacaaggcct gcgaagtggc ccggaggcac aactacttcc 661 ccgggggtgt agctctcatc tgggctacct actatgagag ctgcatcagc tccgagcaga 721 gctgcatcaa cgagtggaac gccatgcagg acctggagtc tacgcggccc gactcccccg 781 cgctatttgt ggacaagccc actgaagggg aaaggaccga gcgcctcatc aaagccaagc 841 tccgaagcat catgatgagc caggatctag aaaatgtgac ttccaaagag attcgtaatg 901 aattagagaa acagatgaat tgtaacttga aggaactcaa ggaatttata gacaatgaga 961 tgctacttat cttgggacag atggacaagc cctcccttat cttcgatcat ctttatctcg 1021 gctctgaatg gaatgcatcc aatctggagg aactgcaggg ctcaggggtt gattacattt 1081 taaatgttac cagagaaatc gataattttt ttcctggctt atttgcatat cataacatcc 1141 gagtctacga tgaagagacc acagacctcc tcgcccactg gaatgaagcg tatcatttta 1201 taaacaaagc gaagaggaac cattccaagt gcctggtgca ttgcaaaatg ggcgtgagtc 1261 gctcggcctc cacagtcata gcctatgcaa tgaaggaatt cggctggcct ctggaaaaag 1321 catataacta tgtaaagcag aagcgcagca tcacgcgccc caacgcgggc tttatgaggc 1381 agctgtctga gtatgaaggc atcttggatg caagcaaaca gcggcacaac aagctgtggc 1441 gtcagcagac agacagcagc ctccagcagc ctgtggatga ccctgcagga cctggcgact 1501 tcttgccaga gaccccagat ggcaccccgg aaagccagct gcccttcttg gatgatgccg 1561 cccagcccgg cttagggccc cccctcccct gctgtttccg gcgactctca gacccccttc 1621 tgccttcccc tgaggatgaa actggcagct tggtccacct ggaggatccg gagagggagg 1681 ctctgttgga ggaagctgct ccacctgcag aggtgcacag gccggccaga cagccccagc 1741 aaggttccgg actctgtgag aaggatgtga agaagaaact agagtttggg agtcccaaag 1801 gtcggagcgg ctccttgctg caggtggagg agacggaaag ggaggagggc ctgggagcag 1861 ggaggtgggg gcagcttcca acccagctcg atcaaaacct gctcaactcg gagaacctaa 1921 acaacaacag caagaggagc tgtcccaacg gcatggagga tgatgctata tttgggatcc 1981 ttaacaaagt gaagccttcc tataaatcct gtgccgactg catgtaccct acagccagcg 2041 gggctcctga ggcctccagg gagcgatgtg aggaccccaa tgctcccgcc atctgcaccc 2101 agccagcctt cctaccccac atcacgtcct cccctgtggc ccacttggcc agcaggtccc 2161 gtgttccgga gaagccagcc tctggcccaa ccgaacctcc cccgttccta ccaccagcag 2221 gctccaggag ggcagacacc agtggccctg gggctggagc tgccctagag ccaccagcca 2281 gccttttgga accttccaga gagaccccaa aagtcctgcc aaagtccctc cttttgaaga 2341 attctcactg tgataagaac cctcccagca cagaagtggt aataaaggaa gaatcgtcac 2401 ccaagaaaga tatgaagcca gccaaggacc tgaggcttct gttcagtaat gaatctgaga 2461 agccgacaac caacagctac ctgatgcagc accaggagtc catcattcag ctgcagaagg 2521 caggcttggt ccgcaagcac accaaagagc tagagcggct gaagagcgtg cctgcagacc 2581 cagcacctcc ctccagggat ggccctgcca gcaggctgga ggccagcatc cccgaggaga 2641 gccaggatcc agccgcgctc cacgagctgg gccccctggt tatgcccagc caggccggga 2701 gtgatgagaa gtcagaggcc gcccccgctt cattggaagg aggctcactg aagagccccc 2761 ctcctttctt ctaccgcctg gaccacacca gtagtttctc aaaagacttt ctgaagacca 2821 tctgctacac ccccacctcc tcttccatga gctccaacct gacccggagt tccagcagcg 2881 atagcatcca cagtgtccgt gggaagcccg ggctggtgaa gcagcggacg caggagattg 2941 agacccggcT CCGGCTGGCG GGCCTCACCG TCTCTTCCCC ACTGAAGCGC TCACACTCTC 3001 TTGCCAAGCT GGGGAGTCTC ACCTTCTCAA CGGAAGACCT GTCCAGTGAG GCTGACCCGT 3061 CCACCGTCGC TGACTCCCAG GACACCACTT TGAGTGAATC TTCCTTCTTG CATGAGCCCC 3121 AGGGAACCCC GAGGGACCCA GCTGCAACCT CCAAACCATC AGGGAAACCC GCCCCAGAAA 3181 ACTTAAAGAG CCCTTCGTGG ATGAGCAAAA GCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 3241 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 3301 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 3361 TCCCTAGGTA ATGCTTTCTC GAACGCGTTA AGTCgacaat caacctctgg attacaaaat 3421 ttgtgaaaga tt