Construct: ORF TRCN0000491310
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019553.2_s317c1
- DNA Barcode:
- TCCTCGGTTTCTGACTGATCCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR20 (2843)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491310
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2843 | GPR20 | G protein-coupled receptor 20 | NM_005293.3 | 99.6% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1152
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgccc tctgtgtctc cagcggggcc ctcggccggg gcagtcccca 121 atgccaccgc agtgacaaca gtgcggacca atgccagcgg gctggaggtg cccctgttcc 181 acctgtttgc ccggctggac gaggagctgc atggcacctt cccaggcctg tggctggcgc 241 tgatggcggt gcacggagcc atcttcctgg cagggctggt gctcaacggg ctggcgctgt 301 acgtcttctg ctgccgcacc cgggccaaga caccctcagt catctacacc atcaacctgg 361 tggtgaccga tctactggta gggctgtccc tgcccacgcg cttcgctgtg tactacggcg 421 ccaggggctg cctgcgctgt gccttcccgc acgtcctcgg ttacttcctc aacatgcact 481 gctccatcct cttcctcacc tgcatctgcg tggaccgcta cctggccatc gtgcggcctg 541 aaggctcccg ccgctgccgc cagcctgcct gtgccagggc cgtgtgcgcc ttcgtgtggc 601 tggccgccgg tgccgtcacc ctgtcggtgc tgggcgtgac aggcagccgg ccctgctgcc 661 gtgtctttgc gctgactgtc ctggagttcc tgctgcctct gctggtcatc agcgtgttta 721 ccggccgcat catgtgtgca ctgtcgcggc cgggtctgct ccgccagggt cgccagcgcc 781 gcgtgcgggc catgcagctc ctgctcacgg tgctcatcat ctttctcgtc tgcttcacgc 841 ccttccacgc ccgccaagtg gccgtggcgc tgtggcccga catgccacac cacacgagcc 901 tcgtggtcta ccacgtggcc gtgaccctca gcagcctcaa cagctgcatg gaccccatcg 961 tcTACTGCTT CGTCACCAGT GGCTTCCAGG CCACCGTCCG AGGCCTCTTC GGCCAGCACG 1021 GAGAGCGTGA GCCCAGCAGC GGTGACGTGG TCAGCATGCA CAGGAGCTCC AAGGGCTCAG 1081 GCCGTCATCA CATCCTCAGT GCCGGCCCTC ACGCCCTCAC CCAGGCCCTG GCTAATGGGC 1141 CCGAGGCTGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCCTCG GTTTCTGACT GATCCCCCAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt