Construct: ORF TRCN0000491334
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020805.2_s317c1
- DNA Barcode:
- AGGCGCCGAGAGCAATCGATATGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CXCR4 (7852)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491334
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_003467.3 | 100% | 100% | |
| 2 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_001008540.2 | 97% | 97.4% | 1_22delinsA;25C>G;27_28insGGGATCAGT |
| 3 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_001348060.2 | 95.7% | 95.7% | 0_1ins45 |
| 4 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_001348059.2 | 91.4% | 90.3% | 16_114del |
| 5 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_001348056.2 | 81.4% | 82% | 1_225del;228_229insGAGGGGATCAGT |
| 6 | mouse | 12767 | Cxcr4 | chemokine (C-X-C motif) rec... | XM_006529113.3 | 85.6% | 89.9% | (many diffs) |
| 7 | mouse | 12767 | Cxcr4 | chemokine (C-X-C motif) rec... | NM_009911.3 | 85.1% | 89.1% | (many diffs) |
| 8 | mouse | 12767 | Cxcr4 | chemokine (C-X-C motif) rec... | XM_006529114.2 | 84.8% | 88.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1128
- ORF length:
- 1056
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagggg atcagtatat acacttcaga taactacacc gaggaaatgg 121 gctcagggga ctatgactcc atgaaggaac cctgtttccg tgaagaaaat gctaatttca 181 ataaaatctt cctgcccacc atctactcca tcatcttctt aactggcatt gtgggcaatg 241 gattggtcat cctggtcatg ggttaccaga agaaactgag aagcatgacg gacaagtaca 301 ggctgcacct gtcagtggcc gacctcctct ttgtcatcac gcttcccttc tgggcagttg 361 atgccgtggc aaactggtac tttgggaact tcctatgcaa ggcagtccat gtcatctaca 421 cagtcaacct ctacagcagt gtcctcatcc tggccttcat cagtctggac cgctacctgg 481 ccatcgtcca cgccaccaac agtcagaggc caaggaagct gttggctgaa aaggtggtct 541 atgttggcgt ctggatccct gccctcctgc tgactattcc cgacttcatc tttgccaacg 601 tcagtgaggc agatgacaga tatatctgtg accgcttcta ccccaatgac ttgtgggtgg 661 ttgtgttcca gtttcagcac atcatggttg gccttatcct gcctggtatt gtcatcctgt 721 cctgctattg cattatcatc tccaagctgt cacactccaa gggccaccag aagcgcaagg 781 ccctcaagac cacagtcatc ctcatcctgg ctttcttcgc ctgttggctg ccttactaca 841 ttgggatcag catcgactcc ttcatccTCC TGGAAATCAT CAAGCAAGGG TGTGAGTTTG 901 AGAACACTGT GCACAAGTGG ATTTCCATCA CCGAGGCCCT AGCTTTCTTC CACTGTTGTC 961 TGAACCCCAT CCTCTATGCT TTCCTTGGAG CCAAATTTAA AACCTCTGCC CAGCACGCAC 1021 TCACCTCTGT GAGCAGAGGG TCCAGCCTCA AGATCCTCTC CAAAGGAAAG CGAGGTGGAC 1081 ATTCATCTGT TTCCACTGAG TCTGAGTCTT CAAGTTTTCA CTCCAGCTAG GACCCAGCTT 1141 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1201 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1261 CTTGTGGAAA GGACGAAGGC GCCGAGAGCA ATCGATATGT ACGCGTTAAG TCgacaatca 1321 acctctggat tacaaaattt gtgaaagatt