Construct: ORF TRCN0000491353
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013562.1_s317c1
- Derived from:
- ccsbBroadEn_09657
- DNA Barcode:
- TTACTTGGGCACTCGCTGAATCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHF13 (148479)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491353
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 148479 | PHF13 | PHD finger protein 13 | NM_153812.3 | 99.8% | 99.6% | 58A>G |
| 2 | human | 148479 | PHF13 | PHD finger protein 13 | XM_011540762.1 | 94.3% | 92.8% | (many diffs) |
| 3 | mouse | 230936 | Phf13 | PHD finger protein 13 | NM_172705.2 | 87.2% | 92% | (many diffs) |
| 4 | mouse | 230936 | Phf13 | PHD finger protein 13 | XM_011250239.2 | 43.5% | 46.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 966
- ORF length:
- 900
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ctctgactct tgcgccgccg ccttccaccc ggaggaatac tcccccagtt 121 gcgagaggcg caggaccgtg gaagacttca acaaattctg cacctttgtc ttggcctatg 181 ctggctacat cccttatccg aaggaggaac tccctttaag gagcagcccc agccctgcta 241 acagcactgc tggtaccatt gacagcgacg gctgggacgc gggtttctca gacatcgcgt 301 cctcagtgcc cttgccagtc tctgaccgct gctttagcca cctgcagcct actctcttgc 361 agcgagccaa gcccagtaac ttcctgctgg acagaaagaa aacggacaag ctgaagaaga 421 agaagaagag gaagcgcagg gacagtgatg cgcctgggaa agaggggtac agggggggct 481 tgctgaagct ggaagccgct gacccctacg tggagacccc cacgagtccc accttgcagg 541 atatccccca ggctcccagc gacccctgct cgggctggga ctccgatact ccctcgagtg 601 gatcttgtgc cactgtgtca ccTGATCAGG TCAAAGAAAT AAAAACTGAA GGCAAACGGA 661 CTATCGTCCG GCAGGGAAAG CAGGTGGTGT TCCGAGATGA GGACAGCACT GGCAATGATG 721 AGGACATCAT GGTGGACTCA GATGACGATT CCTGGGACCT CGTGACCTGC TTCTGCATGA 781 AGCCATTTGC CGGCCGCCCC ATGATCGAGT GTAATGAGTG CCACACCTGG ATTCACCTGT 841 CCTGTGCGAA AATCCGGAAA TCCAATGTTC CAGAAGTGTT TGTCTGCCAA AAGTGCCGGG 901 ACTCCAAGTT TGACATCCGC CGTTCCAACC GCTCGCGGAC GGGCTCCCGG AAGCTGTTCC 961 TGGACTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1021 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCTGTA ACTTGAAAGT ATTTCGATTT 1081 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTACTTGGG CACTCGCTGA ATCCCACGCG 1141 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt