Construct: ORF TRCN0000491378
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020686.2_s317c1
- DNA Barcode:
- ACCAAGCTCTTGTTATCGCAACCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PIK3R4 (30849)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491378
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30849 | PIK3R4 | phosphoinositide-3-kinase r... | NM_014602.3 | 100% | 100% | |
| 2 | mouse | 75669 | Pik3r4 | phosphoinositide-3-kinase r... | NM_001081309.1 | 85.7% | 95.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 4146
- ORF length:
- 4074
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggaaat cagcttgctg gcattgctcc ctcccagatc ctttctgtag 121 agagttattt ttcagatatt catgactttg aatatgataa aagcctgggg agtactcggt 181 tttttaaagt tgctcgagcc aagcaccgag aaggcctggt cgttgtgaag gtttttgcaa 241 ttcaggatcc cacattgcct ttaaccagct ataaacaaga gctggaggaa ctgaaaatca 301 ggcttaattc tgcacagaat tgtctacctt tccagaaagc atcagaaaaa gcatctgaga 361 aagcagctat gctctttagg cagtatgtgc gagacaatct ctatgatcgc atcagtaccc 421 gtccattctt gaataacatt gagaagcgct ggattgcttt ccagatcctg acagctgtgg 481 accaagcaca caaatctgga gttcgtcatg gggacatcaa gactgagaat gtgatggtca 541 ccagttggaa ttgggttctt ctaactgatt ttgccagttt taagcccact tatcttccag 601 aagacaaccc ggcagatttc aattatttct ttgacacatc acggaggaga acttgctata 661 ttgctcctga acgttttgtt gatggtggga tgtttgccac tgagttagaa tatatgagag 721 atccttcaac tccgcttgta gacttaaata gcaatcagag aacaagagga gagttgaaga 781 gagcaatgga catcttttca gcaggttgtg tgatagctga gctttttaca gaaggtgtac 841 cattatttga tctctctcaa cttttggctt atagaaatgg acattttttc cctgaacaag 901 tgctaaataa aattgaagat cacagtatca gagaattggt aactcagatg attcaccgtg 961 agccagataa acgtttagag gcagaagatt acttaaaaca gcagcgtggc aatgcctttc 1021 ctgaaatatt ttacactttt cttcagccct acatggccca gtttgccaag gaaacgtttc 1081 tttctgcaga tgagcgtatt ctggttatac ggaaggattt gggcaacatt attcacaatc 1141 tctgtggaca tgatctgcca gaaaaagccg aaggagagcc taaggaaaat gggctggtta 1201 tcttggtatc tgttataaca tcctgcctac agacccttaa atactgtgat tccaaactag 1261 ctgctttgga actgattctt catttggctc caagattaag tgttgaaatc cttttggatc 1321 gtattactcc atatcttttg catttcagca atgactctgt tcctagggtg agggctgaag 1381 ccttgaggac gttgaccaaa gttcttgctc tcgtcaaaga ggttcctcgt aatgatatca 1441 atatttatcc ggaatacatt ctgccaggca tagcccactt agcccaagat gatgctacta 1501 tcgttagact agcctatgct gaaaacatag ctctgctggc agaaacagct ctgagattcc 1561 tggaattagt acagttaaaa aatcttaata tggaaaatga ccccaataat gaagaaatag 1621 atgaggttac acatccaaat ggaaattatg acacagagct ccaagcctta catgaaatgg 1681 tccagcagaa agttgttact ttgctaagtg accctgaaaa tattgtaaaa caaaccttga 1741 tggaaaatgg aataacacgg ctgtgtgtat tctttggacg tcagaaagcc aacgatgttt 1801 tgttgtccca catgattact ttcctaaatg ataagaatga ttggcatcta cgtggagcat 1861 tttttgatag tatagttggt gttgctgcct atgttggctg gcaaagctcc tcaattctca 1921 agcctctgct gcaacaaggt cttagtgatg ctgaggaatt tgtcattgtg aaagctcttt 1981 atgcccttac ttgtatgtgc cagttaggac tgctacaaaa accccatgtt tacgaatttg 2041 ccagtgatat tgcccccttc ctgtgtcatc ccaatttatg gatacgttat ggtgccgtgg 2101 gatttatcac agtggtagct cgtcaaataa gtacagctga tgtctactgt aaactgatgc 2161 cttatcttga cccatatatt acccaaccaa taatacagat tgaaagaaaa cttgttctgc 2221 tcagtgtttt aaaggaacca gtaagtcgtt ctatatttga ttatgctttg aggtctaaag 2281 atattactag cttgttcaga catcttcaca tgcgtcagaa gaaacgaaat ggttctcttc 2341 ccgactgccc tccgccagag gatcctgcca tagcacagct tctgaagaag ttgctctcac 2401 agggaatgac agaggaagag gaagacaaac ttctggcact gaaagacttc atgatgaaat 2461 ctaataaagc aaaggccaat atagtggacc agagccatct tcatgatagt agtcagaaag 2521 gtgtaattga cttggcagct ttaggcataa ctgggagaca agttgatctt gttaaaacca 2581 aacaagaacc agatgacaaa cgggccagaa aacatgtaaa acaagactca aatgtaaatg 2641 aagaatggaa aagcatgttt gggtcactgg acccaccaaa catgccacag gccctaccta 2701 aagggagtga tcaggaggtg attcagactg ggaaacctcc tcgttccgag tcctctgctg 2761 gcatttgtgt ccctttgtca acttcttcac aggttccaga agtgacaact gtccaaaata 2821 aaaaaccagt aataccggtt ttaagtagta caatcttacc atccacctat cagattcgaa 2881 ttacaacttg taaaactgaa cttcagcaac tcatccagca aaagcgggag cagtgcaatg 2941 ctgagagaat agctaagcag atgatggaaa atgctgaatg ggagagtaaa ccaccaccac 3001 ctggatggcg tcctaaaggg ctgttagttg cccatcttca tgagcataaa tctgctgtga 3061 atcgaattag agtctctgat gaacactcac tttttgcaac atgttcaaat gatggcacag 3121 tgaaaatctg gaacagtcaa aagatggagg ggaagaccac cactaccaga tctattctta 3181 catacagccg aattggagga cgagtcaaga cgctcacatt ctgccaaggc tcccactatt 3241 tagccatagc atctgataat ggtgctgtcc agcttcttgg aattgaggct tctaagctgc 3301 ccaagtctcc taaaatccat cctctacaaa gcagaattct agatcagaag gaggacggtt 3361 gtgttgtgga tatgcatcac ttcaactctg gagcacagtc tgttcttgcc tatgccactg 3421 tgaatggctc tctggttggc tgggacctta ggtcttcaag caatgcgtgg actttaaagc 3481 atgatttaaa gtcgggcctc atcacttcct ttgctgtgga catccaccaa tgctggctct 3541 gcattggtac aagcagtggt accatggctt gttgggacat gaggttccag ttgccaattt 3601 caagtcactg tcatccttcc agggctcgaa tcagacgcct ctcaatgcac cctctgtatc 3661 agtcctgggt gattgcagct gttcagggca acaacgaagt gtccatgtgg gacatggaga 3721 ctggtgacag aagatttact ctctgggcca gcagtgcacc accactttcT GAATTACAGC 3781 CTTCTCCTCA TAGCGTCCAT GGTATCTACT GTAGTCCTGC AGATGGAAAT CCTATCCTAC 3841 TAACAGCTGG CTCAGATATG AAAATAAGGT TTTGGGACTT GGCTTACCCA GAAAGGTCCT 3901 ATGTTGTTGC AGGAAGTACT AGTTCCCCAT CTGTGTCCTA CTACAGGAAA ATAATTGAAG 3961 GCACTGAAGT TGTCCAGGAA ATTCAGAATA AGCAGAAAGT AGGACCAAGT GATGACACCC 4021 CTCGAAGGGG CCCAGAGTCC CTGCCCGTGG GACATCATGA CATCATCACT GATGTCGCCA 4081 CATTCCAGAC CACACAGGGC TTCATCGTAA CTGCTTCTAG AGATGGGATT GTGAAGGTGT 4141 GGAAATAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 4201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 4261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACCAAG CTCTTGTTAT CGCAACCTAC 4321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt