Construct: ORF TRCN0000491380
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019732.2_s317c1
- DNA Barcode:
- CCATGCGCGCTTCGTAACGGTACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IPPK (64768)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491380
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64768 | IPPK | inositol-pentakisphosphate ... | NM_022755.6 | 99.8% | 99.5% | 1126C>T;1473_1474insG |
| 2 | human | 64768 | IPPK | inositol-pentakisphosphate ... | XM_017015041.1 | 80.7% | 75.4% | 1126C>T;1169_1244del;1329_1330ins221 |
| 3 | human | 64768 | IPPK | inositol-pentakisphosphate ... | XR_001746365.1 | 46.7% | (many diffs) | |
| 4 | mouse | 75678 | Ippk | inositol 1,3,4,5,6-pentakis... | NM_199056.3 | 87.4% | 90.6% | (many diffs) |
| 5 | mouse | 75678 | Ippk | inositol 1,3,4,5,6-pentakis... | NM_001276399.1 | 78.2% | 79.1% | (many diffs) |
| 6 | mouse | 75678 | Ippk | inositol 1,3,4,5,6-pentakis... | XM_017315628.1 | 57.9% | 56.6% | (many diffs) |
| 7 | mouse | 75678 | Ippk | inositol 1,3,4,5,6-pentakis... | NR_075085.1 | 42.6% | (many diffs) | |
| 8 | mouse | 75678 | Ippk | inositol 1,3,4,5,6-pentakis... | XR_001780827.1 | 42.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1548
- ORF length:
- 1476
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaagag gggaagatgg acgagaatga atgggggtac cacggagagg 121 gcaataagag cctggtggtg gcccacgcgc agcgctgcgt cgtgctgcgg tttctgaagt 181 ttcctccaaa taggaagaag acctcggaag agatatttca acacctgcag aacatagtgg 241 actttgggaa aaatgtcatg aaggagtttt tgggggagaa ctatgttcat tatggggagg 301 tcgttcagct acctttagag tttgtgaaac agctttgttt aaagatacaa tctgaaagac 361 cagagtctcg ctgtgacaag gacctggata ctctcagtgg ttacgctatg tgccttccta 421 atttaaccag actccaaacc taccgctttg cagagcaccg gccgattctg tgtgtagaga 481 ttaagccaaa atgtgggttt attcctttct cgagtgatgt cacgcatgag atgaagcata 541 aggtctgtcg atactgcatg caccagcacc tcaaggtagc aactgggaag tggaagcaga 601 tcagcaaata ctgtcccctt gatctctact caggaaacaa acagagaatg cactttgcct 661 tgaagagttt gctgcaggag gcacagaaca acttgaagat atttaagaat ggtgagctga 721 tttacggctg caaagatgcc cggagccccg tggctgactg gagcgagctt gcacaccacc 781 tgaagccgtt cttcttccct tccaacggcc tggccagtgg gccccactgc acaagggctg 841 tgatcaggga gctggtgcac gtgatcacac gggtgctgct gagtggctcg gacaagggcc 901 gggcaggcac cctgagtccg gggctcgggc ctcagggccc gcgagtctgc gaagccagcc 961 ctttcagtag gagccttcgc tgccaaggaa aaaacacccc agagcgctcg gggttaccga 1021 agggctgtct tctgtacaaa accctccagg tgcagatgtt ggacctgctg gacatcgaag 1081 gcctctaccc tctgtacaac cgggttgagc gatacctgga agagtttccc gaggagagaa 1141 aaaccttaca aatagatggg ccttatgatg aagcatttta ccagaagctg cttgactttt 1201 ccactgagga tgacgggaca gtggccTTCG CGCTAACGAA GGTGCAGCAG TACCGCGTCG 1261 CCATGACTGC CAAGGACTGC TCCATCATGA TTGCACTGTC TCCCTGTCTG CAGGATGCCA 1321 GCTCTGATCA AAGGCCTGTC GTCCCTTCAT CGAGGTCCAG GTTTGCCTTT TCCGTGTCTG 1381 TGCTGGACCT TGACCTCAAG CCCTACGAGA GCATTCCCCA TCAGTATAAA CTGGACGGCA 1441 AGATCGTCAA CTATTATTCA AAGACTGTAC GTGCCAAAGA CAACGCCGTG ATGTCGACTC 1501 GGTTCAAGGA AAGCGAAGAT TGCACATTAG TTCTCCACAA GGTCGACCCA GCTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA CCATGCGCGC TTCGTAACGG TACAACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt