Construct: ORF TRCN0000491388
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017261.1_s317c1
- Derived from:
- ccsbBroadEn_14611
- DNA Barcode:
- ACATGCTGGCTAGCAGTGCCTTTA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAZ1 (1617)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491388
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1617 | DAZ1 | deleted in azoospermia 1 | NM_004081.5 | 80.3% | 78.3% | (many diffs) |
| 2 | human | 57135 | DAZ4 | deleted in azoospermia 4 | NM_001005375.2 | 76.2% | 56.3% | (many diffs) |
| 3 | human | 1617 | DAZ1 | deleted in azoospermia 1 | XM_011531482.2 | 73.7% | 71.7% | (many diffs) |
| 4 | human | 57135 | DAZ4 | deleted in azoospermia 4 | XM_005262602.1 | 71.4% | 47.2% | (many diffs) |
| 5 | human | 1617 | DAZ1 | deleted in azoospermia 1 | XM_011531483.1 | 68.6% | 49.8% | (many diffs) |
| 6 | human | 57135 | DAZ4 | deleted in azoospermia 4 | XM_011531509.3 | 64.8% | 41.8% | (many diffs) |
| 7 | human | 57055 | DAZ2 | deleted in azoospermia 2 | NM_001005785.2 | 61.4% | 29.6% | (many diffs) |
| 8 | human | 57055 | DAZ2 | deleted in azoospermia 2 | NM_020363.3 | 61.2% | 28.8% | (many diffs) |
| 9 | human | 57054 | DAZ3 | deleted in azoospermia 3 | NM_020364.3 | 57.2% | 33.5% | (many diffs) |
| 10 | human | 57055 | DAZ2 | deleted in azoospermia 2 | NM_001005786.2 | 38.3% | 36.3% | (many diffs) |
| 11 | human | 57135 | DAZ4 | deleted in azoospermia 4 | NM_020420.3 | 37.5% | 35.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1908
- ORF length:
- 1839
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctgctgca aatcctgaga ctccaaactc aaccatctcc agagaggcca 121 gcacccagtc ttcatcagct gcagctagcc aaggctgggt gttaccagaa ggcaaaatcg 181 tgccaaacac tgtttttgtt ggtggaattg atgctaggat ggatgaaact gagattggaa 241 gctgctttgg tagatacggt tcagtgaaag aagtgaagat aatcacgaat cgaactggtg 301 tgtccaaagg ctatggattt gtttcgtttg ttaatgacgt ggatgtccag aagatagtag 361 gatcacagat acatttccat ggtaaaaagc tgaagctggg ccctgcaatc aggaaacaaa 421 agttatgtgc tcgtcatgtg cagccacgtc ctttggtagt taatcctcct cctccaccac 481 agtttcagaa cgtctggcgg aatccaaaca ctgaaaccta cctgcagccc caaatcacgc 541 cgaatcctgt aactcagcac gttcagtctg ctgcaaatcc tgagactcca aactcaacca 601 tctccagaga ggccagcacc cagtcttcat cagctgcagc tagccaaggc tgggtgttac 661 cagaaggcaa aatcgtgcca aacactgttt ttgttggtgg aattgatgct aggatggatg 721 aaactgagat tggaagctgc tttggtagat acggttcagt gaaagaagtg aagataatca 781 cgaatcgaac tggtgtgtcc aaaggctatg gatttgtttc gtttgttaat gacgtggatg 841 tccagaagat agtaggatca cagatacatt tccatggtaa aaagctgaag ctgggccctg 901 caatcaggaa acaaaagtta tgtgctcgtc atgtgcagcc acgtcctttg gtagttaatc 961 ctcctcctcc accacagttt cagaacgtct ggcggaatcc aaacactgaa acctacctgc 1021 agccccaaat cacgccgaat cctgtaactc agcacgttca gtctgctgca aatcctgaga 1081 ctccaaactc aaccatctcc agagaggcca gcacccagtc ttcatcagct gcagctagcc 1141 aaggctgggt gttaccagaa ggcaaaatcg tgccaaacac tgtttttgtt ggtggaattg 1201 atgctaggat ggatgaaact gagattggaa gctgctttgg tagatacggt tcagtgaaag 1261 aagtgaagat aatcacgaat cgaactggtg tgtccaaagg ctatggattt gtttcgtttg 1321 ttaatgacgt ggatgtccag aagatagtag gatcacagat acatttccat ggtaaaaagc 1381 tgaagctggg ccctgcaatc aggaaacaaa agttatgtgc tcgtcatgtg cagccacgtc 1441 ctttggtagt taatcctcct cctccaccac agtttcagaa cgtctggcgg aatccaaaca 1501 ctgaaaccta cctgcagccc caaatcacgc cgaatcctgt aactcagcac gttcaggctt 1561 actcTGCTTA TCCACATTCA CCAGGTCAGG TCATCACTGG ATGTCAGTTG CTTGTATATA 1621 ATTATCAGGA ATATCCTACT TATCCCGATT CAGCATTTCA GGTCACCACT GGATATCAGT 1681 TGCCTGTATA TAATTATCAG CCATTTCCTG CTTATCCAAG ATCACCATTT CAGGTCACTG 1741 CTGGATATCA GTTGCCTGTA TATAATTATC AGGCATTTCC TGCTTATCCA AATTCACCAT 1801 TTCAAGTCGC CACTGGATAT CAGTTCCCTG TATGTGAAAT TTGCAAAATA CTGGTATTAA 1861 AGAACGCTGC AGCTTTTTTA TGTCACTCAA AGGTTAATCG GAGTATCTGC CAACTTTCTT 1921 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 1981 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 2041 TGGAAAGGAC GAACATGCTG GCTAGCAGTG CCTTTAACGC GTTAAGTCga caatcaacct 2101 ctggattaca aaatttgtga aagatt