Construct: ORF TRCN0000491391
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021513.2_s317c1
- DNA Barcode:
- GAAAACGCAGAACTGTAAAGGTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PSAT1 (29968)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491391
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29968 | PSAT1 | phosphoserine aminotransfer... | NM_058179.4 | 100% | 100% | |
| 2 | human | 29968 | PSAT1 | phosphoserine aminotransfer... | NM_021154.5 | 87.5% | 87.5% | 869_870ins138 |
| 3 | mouse | 107272 | Psat1 | phosphoserine aminotransfer... | NM_177420.2 | 86.3% | 92.4% | (many diffs) |
| 4 | mouse | 107272 | Psat1 | phosphoserine aminotransfer... | NM_001205339.1 | 83.9% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1179
- ORF length:
- 1110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggacgccccc aggcaggtgg tcaactttgg gcctggtccc gccaagctgc 121 cgcactcagt gttgttagag atacaaaagg aattattaga ctacaaagga gttggcatta 181 gtgttcttga aatgagtcac aggtcatcag attttgccaa gattattaac aatacagaga 241 atcttgtgcg ggaattgcta gctgttccag acaactataa ggtgattttt ctgcaaggag 301 gtgggtgcgg ccagttcagt gctgtcccct taaacctcat tggcttgaaa gcaggaaggt 361 gtgctgacta tgtggtgaca ggagcttggt cagctaaggc cgcagaagaa gccaagaagt 421 ttgggactat aaatatcgtt caccctaaac ttgggagtta tacaaaaatt ccagatccaa 481 gcacctggaa cctcaaccca gatgcctcct acgtgtatta ttgcgcaaat gagacggtgc 541 atggtgtgga gtttgacttt atacccgatg tcaagggagc agtactggtt tgtgacatgt 601 cctcaaactt cctgtccaag ccagtggatg tttccaagtt tggtgtgatt tttgctggtg 661 cccagaagaa tgttggctct gctggggtca ccgtggtgat tgtccgtgat gacctgctgg 721 ggtttgccct ccgagagtgc ccctcggtcc tggaatacaa ggtgcaggct ggaaacagct 781 ccttgtacaa cacgcctcca tgtttcagca tctacgtcat gggcttggtt ctggagtgga 841 ttaaaaacaa tggaggtgcc gcggccatgg agaagcttag ctccatcaaa tctcaaacaa 901 tttatgagat tattgataat tctcaaggat tctacgtttg tccagtGGAG CCCCAAAATA 961 GAAGCAAGAT GAATATTCCA TTCCGCATTG GCAATGCCAA AGGAGATGAT GCTTTAGAAA 1021 AAAGATTTCT TGATAAAGCT CTTGAACTCA ATATGTTGTC CTTGAAAGGG CATAGGTCTG 1081 TGGGAGGCAT CCGGGCCTCT CTGTATAATG CTGTCACAAT TGAAGACGTT CAGAAGCTGG 1141 CCGCCTTCAT GAAAAAATTT TTGGAGATGC ATCAGCTATA GAACCCAGCT TTCTTGTACA 1201 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1261 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1321 AGGACGAGAA AACGCAGAAC TGTAAAGGTC GACGCGTTAA GTCgacaatc aacctctgga 1381 ttacaaaatt tgtgaaagat t