Construct: ORF TRCN0000491414
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021346.2_s317c1
- DNA Barcode:
- CCAACCCCGCAGAATAAGCCCCGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CHRNA1 (1134)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491414
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1134 | CHRNA1 | cholinergic receptor nicoti... | NM_000079.4 | 57.6% | 55.7% | (many diffs) |
| 2 | human | 1134 | CHRNA1 | cholinergic receptor nicoti... | NM_001039523.3 | 54.6% | 52.9% | (many diffs) |
| 3 | human | 1134 | CHRNA1 | cholinergic receptor nicoti... | XM_017003257.1 | 54.1% | 52.8% | (many diffs) |
| 4 | human | 1134 | CHRNA1 | cholinergic receptor nicoti... | XM_017003256.1 | 51.4% | 50.2% | (many diffs) |
| 5 | mouse | 11435 | Chrna1 | cholinergic receptor, nicot... | NM_007389.5 | 51.7% | 52.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 879
- ORF length:
- 807
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagccc tggcctctcc tcctgctctt tagcctttgc tcagctggcc 121 tcgtcctggg ctccgaacat gagacccgtc tggtggcaaa gctatttaaa gactacagca 181 gcgtggtgcg gccagtggaa gaccaccgcc aggtcgtgga ggtcaccgtg ggcctgcagc 241 tgatacagct catcaatgtg gatgaagtaa atcagatcgt gacaaccaat gtgcgtctga 301 aacagcaatg ggtggattac aacctaaaat ggaatccaga tgactatggc ggtgtgaaaa 361 aaattcacat tccttcagaa aagatctggc gcccagacct tgttctctat aacaatgcag 421 atggtgactt tgctattgtc aagttcacca aagtgctcct gcagtacact ggccacatca 481 cgtggacacc tccagccatc tttaaaagct actgtgagat catcgtcacc cactttccct 541 ttgatgaaca gaactgcagc atgaagctgg gcacctggac cTACGACGGC TCTGTCGTGG 601 CCATCAACCC GGAAAGCGAC CAGCCAGACC TGAGCAACTT CATGGAGAGC GGGGAGTGGG 661 TGATCAAGGA GTCCCGGGGC TGGAAGCACT CCGTGACCTA TTCCTGCTGC CCCGACACCC 721 CCTACCTGGA CATCACCTAC CACTTCGTCA TGCAGCGCCT GCCCCTCTAC TTCATCGTCA 781 ACGTCATCAT CCCCTGCCTG CTCTTCTCCT TCTTAACTGG CCTGGTATTC TACCTGCCCA 841 CAGACTCAGG TGGGTGTGGT TGCCATGACT GCTGCTGCTA GAACCCAGCT TTCTTGTACA 901 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 961 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1021 AGGACGACCA ACCCCGCAGA ATAAGCCCCG AACGCGTTAA GTCgacaatc aacctctgga 1081 ttacaaaatt tgtgaaagat t