Construct: ORF TRCN0000491428
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020817.2_s317c1
- DNA Barcode:
- GCAAGATTTCGAATTCACCCCTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADGRF2 (222611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491428
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222611 | ADGRF2 | adhesion G protein-coupled ... | NM_153839.7 | 62.8% | 60.9% | (many diffs) |
| 2 | human | 222611 | ADGRF2 | adhesion G protein-coupled ... | NM_001368115.2 | 58.5% | 58.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1320
- ORF length:
- 1248
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggcacc accatatctg gagataacat tggaaaaaat ttcacttttt 121 cgatgagaat taatgatacc agcaatgaag tcactgggag agtgttgatc agcagagatg 181 aacttcggaa ggtgccttcc ccttctcagg tcatcagcat tgcatttcca actattgggg 241 ctattttgga agccagtctt ttggaaaatg ttactgtaaa tgggcttgtc ctgtctgcca 301 ttttgcccaa ggaacttaaa agaatctcac tgatttttga aaagatcagc aagtcagagg 361 agaggaggac acagtgtgtt ggctggcact ctgtggagaa cagatgggac cagcaggcct 421 gcaaaatgat tcaagaaaac tcccagcaag ctgtttgcaa atgtaggcca agtgaattgt 481 ttacctcttt ctcaattctt atgtcacctc acatcttaga gagtctgatt ctgacttaca 541 tcacatatgt aggcctgggc atttctattt gcagcctgat cctttgcttg tccgttgagg 601 tcctagtctg gagccaagtg acaaagacag agatcaccta tttacgccat gtgtgcattg 661 ttaacattgc agccactttg ctgatggcag atgtgtggtt cattgtggct tcctttctta 721 gtggcccaat aacacaccac aagggatgtg tggcagccac attttttgtt catttctttt 781 acctttctgt atttttctgg atgcttgcca aggcactcct tatcctctat ggaatcatga 841 ttgttttcca taccttgccc aagtcagtcc tggtggcatc tctgttttca gtgggctatg 901 gatgcccttt ggccattgct gccatcactg ttgctgccac tgaacctggc aaaggctatc 961 tacgacctga gatctgctgg ctcaactggg acatgaccaa agccctcctg gccTTCGTGA 1021 TCCCAGCTTT GGCCATCGTG GTAGTAAACC TGATCACAGT CACACTGGTG ATTGTCAAGA 1081 CCCAGCGAGC TGCCATTGGC AATTCCATGT TCCAGGAAGT GAGAGCCATT GTGAGAATCA 1141 GCAAGAACAT CGCCATCCTC ACACCACTTC TGGGACTGAC CTGGGGATTT GGAGTAGCCA 1201 CTGTCATCGA TGACAGATCC CTGGCCTTCC ACATTATCTT CTCCCTGCTC AATGCATTCC 1261 AGGTAAGTCC AGATGCTTCT GACCAAGTGC AAAGTGAGAG AATTCATGAA GATGTTCTGT 1321 AGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1381 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1441 GGCTTTATAT ATCTTGTGGA AAGGACGAGC AAGATTTCGA ATTCACCCCT CCACGCGTTA 1501 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt