Construct: ORF TRCN0000491440
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020700.2_s317c1
- DNA Barcode:
- CAGCCTCTGCTGTACTATCGTAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STKLD1 (169436)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491440
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 169436 | STKLD1 | serine/threonine kinase lik... | NM_153710.5 | 99.9% | 100% | 1371T>C |
| 2 | human | 169436 | STKLD1 | serine/threonine kinase lik... | NR_103997.2 | 39.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2112
- ORF length:
- 2040
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcttggg ccagggtcca atcgcaggcg ccccacgcag ggggagcgag 121 gcccagggtc ccccggagag cccatggaga agtaccaggt tttgtaccag ctgaatcctg 181 gggccttggg ggtgaacctg gtggtggagg aaatggaaac caaagtcaag catgtgataa 241 agcaggtgga atgcatggat gaccattacg ccagtcaggc cctggaggag ctgatgccac 301 tgctgaagct gcggcacgcc cacatctctg tgtaccagga gctgttcatc acgtggaatg 361 gggagatctc ttctctgtac ctctgcctgg tgatggagtt caatgagctc agcttccagg 421 aggtcattga ggataagagg aaggcaaaga aaatcattga ctctgagtgg atgcagaatg 481 tgctgggcca ggtgctggac gcgctggaat acctgcacca tttggacatc atccacagga 541 atctcaaacc ctccaacatc atcctcatca gcagtgacca ctgcaaactg caggacctga 601 gttccaatgt gctaatgaca gacaaagcca aatggaatat tcgtgcggag gaagacccct 661 ttcgtaagtc ctggatggcc cctgaagccc tcaacttctc cttcagccag aaatcagaca 721 tctggtccct gggctgcatc attctggaca tgaccagctg ctccttcatg gatggcacag 781 aagccatgca tctgcggaag tccctccgcc agagcccagg cagcctgaag gccgtcctga 841 agacaatgga ggagaagcag atcccggatg tggaaacctt caggaatctt ctgcccttga 901 tgctccagat cgacccctcg gatcgaataa cgataaagga cgtggtgcac atcaccttct 961 tgagaggctc cttcaagtcc tcgtgcgtct ctctgaccct gcaccggcag atggtgcctg 1021 cgtccatcac cgacatgctg ttagaaggca acgtggccag cattttagag gtcatgcaga 1081 aattctctgg ctggcccgaa gtccagctca gggccatgaa gaggcttctg aaaatgcctg 1141 cagatcagct aggtctgccg tggcccccgg agctggtgga ggtggtggtc acgaccatgg 1201 agctacatga cagggtcctc gatgtccagc tgtgtgcctg ctccctgctg ctgcacctcc 1261 tgggccaagc gctggtgcac cacccggaag ccaaggctcc ctgcaaccaa gccatcacct 1321 ccaccctgct gagtgctctt cagagccacc ccgaggagga gccacttctt gtcatggtct 1381 acagcctgct agccatcacc acaacccagg agtcagagtc actgtcagag gagctgcaga 1441 acgctgggct gctggagcac atcctggagc acctcaacag ctccctcgaa agcagggacg 1501 tctgcgccag cggcctgggc ctgctctggg ccctcctgct ggacggtatc attgtgaaca 1561 aggccccctt ggagaaggtc ccggacctca tcagccaggt gttggccacc taccctgcgg 1621 atggggaaat ggcagaagcc agctgcggag tcttctggct gctgtccctg ctgggctgca 1681 tcaaggagca gcagtttgaa caagtggtgg cgctgctcct gcaaagcatc cggcTGTGCC 1741 AGGACAGAGC CCTGCTGGTG AACAATGCCT ACCGGGGACT GGCCAGCCTG GTGAAGGTGT 1801 CAGAGCTGGC GGCCTTCAAG GTGGTGGTGC AGGAGGAGGG CGGCAGTGGC CTCAGCCTCA 1861 TCAAGGAGAC CTACCAGCTC CACAGGGACG ACCCGGAGGT GGTGGAGAAC GTGGGCATGC 1921 TGCTGGTCCA CCTGGCTTCC TATGAGGAGA TCCTGCCGGA GCTGGTGTCC AGTAGTATGA 1981 AGGCCCTGCT CCAGGAGATC AAGGAGCGCT TCACCTCCAG CCTGGTGAGT GACAGCAGCG 2041 CCTTCAGCAA ACCAGGCCTC CCTCCAGGTG GAAGCCCCCA GCTGGGGTGC ACCACGTCTG 2101 GGGGACTGGA ATAGAACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2161 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2221 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CAGCCTCTGC TGTACTATCG 2281 TAAGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt