Construct: ORF TRCN0000491444
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010617.2_s317c1
- Derived from:
- ccsbBroadEn_06767
- DNA Barcode:
- CGAATCGACCCGTTCATGTGCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRG2 (5553)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491444
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5553 | PRG2 | proteoglycan 2, pro eosinop... | NM_001302926.2 | 99.5% | 99.5% | 13T>C;549A>G;616C>T |
| 2 | human | 5553 | PRG2 | proteoglycan 2, pro eosinop... | NM_001302927.2 | 99.5% | 99.5% | 13T>C;549A>G;616C>T |
| 3 | human | 5553 | PRG2 | proteoglycan 2, pro eosinop... | NM_002728.6 | 99.5% | 99.5% | 13T>C;549A>G;616C>T |
| 4 | human | 5553 | PRG2 | proteoglycan 2, pro eosinop... | NM_001243245.3 | 94.5% | 94.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 732
- ORF length:
- 666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa actcccccta cttctggctc ttctatttgg ggcagtttct gctcttcatc 121 taaggtctga gacttccacc tttgagaccc ctttgggtgc taagacgctg cctgaggatg 181 aggagacacc agagcaggag atggaggaga ccccttgcag ggagctggag gaagaggagg 241 agtggggctc tggaagtgaa gatgcctcca agaaagatgg ggctgttgag tctatctcag 301 tgccagatat ggtggacaaa aaccttacgt gtcctgagga agaggacaca gtaaaagtgg 361 tgggcatccc tgGGTGCCAG ACCTGCCGCT ACCTCCTGGT GAGAAGTCTT CAGACGTTTA 421 GTCAAGCTTG GTTTACTTGC CGGAGGTGCT ACAGGGGCAA CCTGGTTTCC ATCCACAACT 481 TCAATATTAA TTATCGAATC CAGTGTTCTG TCAGCGCGCT CAACCAGGGT CAAGTCTGGA 541 TTGGAGGCAG GATCACAGGC TCGGGTCGCT GCAGACGCTT TCAGTGGGTT GACGGCAGCC 601 GCTGGAACTT TGCGTACTGG GCTGCTCACC AGCCCTGGTC CCGCGGTGGT CACTGCGTGG 661 CCCTGTGTAC CCGAGGAGGC TACTGGCGTC GAGCCCACTG CCTCAGAAGA CTTCCTTTCA 721 TCTGTTCCTA CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 781 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 841 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGA ATCGACCCGT TCATGTGCAT 901 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t