Construct: ORF TRCN0000491448
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002003.1_s317c1
- Derived from:
- ccsbBroadEn_07358
- DNA Barcode:
- CCATTGCGAACCTGAGTTGGGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJA3 (9093)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491448
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9093 | DNAJA3 | DnaJ heat shock protein fam... | NM_005147.6 | 99.7% | 99.7% | 207T>C;223A>T;1083T>A |
| 2 | human | 9093 | DNAJA3 | DnaJ heat shock protein fam... | NM_001135110.3 | 93.6% | 93.3% | (many diffs) |
| 3 | human | 9093 | DNAJA3 | DnaJ heat shock protein fam... | NM_001286516.1 | 61.8% | 55.8% | (many diffs) |
| 4 | mouse | 83945 | Dnaja3 | DnaJ heat shock protein fam... | NM_023646.4 | 86.9% | 87.5% | (many diffs) |
| 5 | mouse | 83945 | Dnaja3 | DnaJ heat shock protein fam... | NM_001135112.1 | 81.2% | 81.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1506
- ORF length:
- 1440
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcgcggtgc tccacacgct ggttgctggt ggttgtgggg accccgcggc 121 tgccggctat atcgggtaga ggggcccggc cgcccaggga gggcgtggtg ggggcatggc 181 tgagccgcaa gctgagcgtc cccgcctttg cgtcttccct gacctcttgc ggcccccgag 241 cgctgctgac attgagacct ggtgtcagcc tcacaggaac aaaacattac cctttcattt 301 gtactgcctc cttccacacg agtgcccctt tggccaaaga agattattat cagatattag 361 gagtgcctcg aaatgccagc cagaaagaga tcaagaaagc ctattatcag cttgccaaga 421 agtatcaccc tgacacaaat aaggatgatc ccaaagccaa ggagaagttc tcccagctgg 481 cagaagccta tgaggttttg agtgatgagg tgaagaggaa gcagtacgat gcctacggct 541 ctgcaggctt cgatcctggg gccagcggct cccagcatag ctactggaag ggaggcccca 601 ctgtggaccc cgaggagctg ttcaggaaga tctttggcga gttctcatcc tcttcatttg 661 gagatttcca gaccgtgttt gatcagcctc aggaatactt catggagttg acattcaatc 721 aagctgcaaa gggggtcaac aaggagttca ccgtgaacat catggacacg tgtgagcgct 781 gcaacggcaa ggggaacgag cccggcacca aggtgcagca ttgccactac tgtggcggct 841 ccggcatgga aaccatcaac acaggccctt ttgtgatgcg ttccacgtgt aggagatgtg 901 gtggccgcgg ctccatcatc atatcgccct gtgtggtctg caggggagca ggacaagcca 961 agcagaaaaa gcgagtgatg atccctgtgc ctgcaggagt cgaggatggc cagaccgtga 1021 ggatgcctgt gggaaaaagg gaaattttca ttacgttcag ggtgcagaaa agccctgtgt 1081 tccggaggga cggcgcagac atccactccg acctctttat ttctatagct caggctcttc 1141 ttgggggaac agccagagcc cagggcctgt acgagacgat caacgtgacg atcccccctg 1201 ggacTCAGAC AGACCAGAAG ATTCGGATGG GTGGGAAAGG CATCCCCCGG ATTAACAGCT 1261 ACGGCTACGG AGACCACTAC ATCCACATCA AGATACGAGT TCCAAAGAGG CTAACGAGCC 1321 GGCAGCAGAG CCTGATCCTG AGCTACGCCG AGGACGAGAC AGATGTGGAG GGGACGGTGA 1381 ACGGCGTCAC CCTCACCAGC TCTGGTGGCA GCACCATGGA TAGCTCCGCA GGAAGCAAGG 1441 CTAGGCGTGA GGCTGGGGAG GACGAGGAGG GATTCCTTTC CAAACTTAAG AAAATGTTTA 1501 CCTCATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1561 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1621 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCATTGCGA ACCTGAGTTG GGCCCACGCG 1681 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt