Construct: ORF TRCN0000491460
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013027.1_s317c1
- Derived from:
- ccsbBroadEn_07459
- DNA Barcode:
- AATACCCGAACTCTTTGTCATTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SOCS5 (9655)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491460
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9655 | SOCS5 | suppressor of cytokine sign... | NM_014011.4 | 99.6% | 99.4% | (many diffs) |
| 2 | human | 9655 | SOCS5 | suppressor of cytokine sign... | NM_144949.3 | 99.6% | 99.4% | (many diffs) |
| 3 | mouse | 56468 | Socs5 | suppressor of cytokine sign... | NM_019654.2 | 87% | 93.8% | (many diffs) |
| 4 | mouse | 56468 | Socs5 | suppressor of cytokine sign... | XM_006524671.3 | 87% | 93.8% | (many diffs) |
| 5 | mouse | 56468 | Socs5 | suppressor of cytokine sign... | XM_006524672.3 | 87% | 93.8% | (many diffs) |
| 6 | mouse | 56468 | Socs5 | suppressor of cytokine sign... | XM_006524674.3 | 87% | 93.8% | (many diffs) |
| 7 | mouse | 56468 | Socs5 | suppressor of cytokine sign... | XM_006524675.3 | 87% | 93.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1674
- ORF length:
- 1608
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga taaagtggga aaaatgtgga ataacttcaa atacaggtgt cagaatctct 121 tcggtcatga gggaggaagc cgtagtgaaa atgtggacat gaactccaac agatgtttgt 181 ctgtcaaaga gaaaaacatc agcataggag actcaactcc tcagcaacaa agcagtccct 241 taagagaaaa tattgcctta caactgggat taagcccttc gaagaattct tcaaggagaa 301 atcaaaattg tgccacagaa atccctcaaa ttgttgaaat aagcatcgaa aaggataatg 361 attcttgtgt tacgccagga acaagacttg cacgaagaga ttcctactct cgacatgctc 421 catggggtgg gaagaaaaaa cattcctgtt ctacaaagac ccagagttca ttggatgctg 481 ataaaaagtt tggtagaact cgaagtggac ttcaaaggag agagaggcgc tacggcgtaa 541 gttctgtaca cgacatggac agtgtttcca gcagaactgt aggaagtcgc tctctaagac 601 agaggttgca ggatactgtg ggcttgtgtt ttcccatgag aacttacagc aagcagtcaa 661 agcctctctt ttccaataaa agaaaaatcc atctctctga attaatgctt gagaaatgcc 721 cttttcctgc tggctcagat ttagcccaaa aatggcattt gattaaacag catacagctc 781 ctgtgagccc acattcaaca ttttttgata catttgatcc atctttggtt tctacagaag 841 atgaagaaga taggcttaga gagagaaggc ggcttagtat tgaagaaggg gttgatcccc 901 ctcccaatgc acaaatacat acatttgaag ctactgcaca ggttaatcca ttatataaac 961 tgggaccaaa attagctcct ggaatgactg aaataagtgg ggacagttct gcaattccac 1021 aagctaattg tgactcggaa gaggatacaa ccaccctgtg tttgcagtca cggaggcaga 1081 agcagcgtca gatatctgga gacagccata cccatgttag cagacaggga gcttggaaag 1141 tccacacaca gattgattac atacactgcc tcgtgcctga tttgcttcaa attacaggga 1201 atccctgtta ctggggagtg atggaccgtt atgaagcaga agcccttctc gaagggaaac 1261 ctgaaggcac gtttttgctc agggactctg cgcaagagga ctacctcttc tctgtgagct 1321 tccgccgcta caacagatcc ctgcatgccc gaattgagca gtggaatcac aactttagtt 1381 tcgacgccca tgacccgtgt gtatttcact cctccactgt aacgggactt ttagaacatt 1441 ataaagatcc cagttcgtgc atgttttttg aaccattgct tactatatca ctaaatatga 1501 ctttcccttt tagcctgcag tatatctgtc gcgcggtaat ctgcaggtgc actacgtatg 1561 atggaattga tgggctccct ctaccctcaa tgttacagga ttttttaaaa gagtatcatt 1621 ataaacaaaa agttagagtt cgctggttgg aacgagaacc agtcaagGAC AGCTTGCCAA 1681 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1741 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1801 TATCTTGTGG AAAGGACGAA ATACCCGAAC TCTTTGTCAT TGAACGCGTT AAGTCgacaa 1861 tcaacctctg gattacaaaa tttgtgaaag att