Construct: ORF TRCN0000491462
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000400.2_s317c1
- Derived from:
- ccsbBroadEn_13895
- DNA Barcode:
- GTGGTCCAATTAAGTTACATCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LTF (4057)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491462
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4057 | LTF | lactotransferrin | NM_002343.6 | 99.7% | 99.7% | 66_67insAGA;1610T>A;1894T>C |
| 2 | human | 4057 | LTF | lactotransferrin | NM_001321121.2 | 99.4% | 99.4% | (many diffs) |
| 3 | human | 4057 | LTF | lactotransferrin | NM_001321122.1 | 97.8% | 97.7% | (many diffs) |
| 4 | human | 4057 | LTF | lactotransferrin | NM_001199149.2 | 93.5% | 93.5% | 0_1ins135;1478T>A;1762T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2199
- ORF length:
- 2133
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa acttgtcttc ctcgtcctgc tgttcctcgg ggccctcgga ctgtgtctgg 121 ctggccgtag gagaaggagt gttcagtggt gcgccgtatc ccaacccgag gccacaaaat 181 gcttccaatg gcaaaggaat atgagaaaag tgcgtggccc tcctgtcagc tgcataaaga 241 gagactcccc catccagtgt atccaggcca ttgcggaaaa cagggccgat gctgtgaccc 301 ttgatggtgg tttcatatac gaggcaggcc tggcccccta caaactgcga cctgtagcgg 361 cggaagtcta cgggaccgaa agacagccac gaactcacta ttatgccgtg gctgtggtga 421 agaagggcgg cagctttcag ctgaacgaac tgcaaggtct gaagtcctgc cacacaggcc 481 ttcgcaggac cgctggatgg aatgtcccta tagggacact tcgtccattc ttgaattgga 541 cgggtccacc tgagcccatt gaggcagctg tggccaggtt cttctcagcc agctgtgttc 601 ccggtgcaga taaaggacag ttccccaacc tgtgtcgcct gtgtgcgggg acaggggaaa 661 acaaatgtgc cttctcctcc caggaaccgt acttcagcta ctctggtgcc ttcaagtgtc 721 tgagagacgg ggctggagac gtggctttta tcagagagag cacagtgttt gaggacctgt 781 cagacgaggc tgaaagggac gagtatgagt tactctgccc agacaacact cggaagccag 841 tggacaagtt caaagactgc catctggccc gggtcccttc tcatgccgtt gtggcacgaa 901 gtgtgaatgg caaggaggat gccatctgga atcttctccg ccaggcacag gaaaagtttg 961 gaaaggacaa gtcaccgaaa ttccagctct ttggctcccc tagtgggcag aaagatctgc 1021 tgttcaagga ctctgccatt gggttttcga gggtgccccc gaggatagat tctgggctgt 1081 accttggctc cggctacttc actgccatcc agaacttgag gaaaagtgag gaggaagtgg 1141 ctgcccggcg tgcgcgggtc gtgtggtgtg cggtgggcga gcaggagctg cgcaagtgta 1201 accagtggag tggcttgagc gaaggcagcg tgacctgctc ctcggcctcc accacagagg 1261 actgcatcgc cctggtgctg aaaggagaag ctgatgccat gagtttggat ggaggatatg 1321 tgtacactgc aggcaaatgt ggtttggtgc ctgtcctggc agagaactac aaatcccaac 1381 aaagcagtga ccctgatcct aactgtgtgg atagacctgt ggaaggatat cttgctgtgg 1441 cggtggttag gagatcagac actagcctta cctggaactc tgtgaaaggc aagaagtcct 1501 gccacaccgc cgtggacagg actgcaggct ggaatatccc catgggcctg ctcttcaacc 1561 agacgggctc ctgcaaattt gatgaatatt tcagtcaaag ctgtgcccct gggtctgacc 1621 cgagatctaa tctctgtgct ctgtgtattg gcgacgagca gggtgagaat aagtgcgagc 1681 ccaacagcaa cgagagatac tacggctaca ctggggcttt ccggtgcctg gctgagaatg 1741 ctggagacgt tgcatttgtg aaagatgtca ctgtcttgca gaacactgat ggaaataaca 1801 atgaggcatg ggctaaggat ttGAAGCTGG CAGACTTTGC GCTGCTGTGC CTCGATGGCA 1861 AACGGAAGCC TGTGACTGAG GCTAGAAGCT GCCATCTTGC CATGGCCCCG AATCATGCCG 1921 TGGTGTCTCG GATGGATAAG GTGGAACGCC TGAAACAGGT GCTGCTCCAC CAACAGGCTA 1981 AATTTGGGAG AAATGGATCT GACTGCCCGG ACAAGTTTTG CTTATTCCAG TCTGAAACCA 2041 AAAACCTTCT GTTCAATGAC AACACTGAGT GTCTGGCCAG ACTCCATGGC AAAACAACAT 2101 ATGAAAAATA TTTGGGACCA CAGTATGTCG CAGGCATTAC TAATCTGAAA AAGTGCTCAA 2161 CCTCCCCCCT CCTGGAAGCC TGTGAATTCC TCAGGAAGTA CCTAACTTTC TTGTACAAAG 2221 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2281 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2341 ACGAGTGGTC CAATTAAGTT ACATCTTAAC GCGTTAAGTC gacaatcaac ctctggatta 2401 caaaatttgt gaaagatt