Construct: ORF TRCN0000491481
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017750.1_s317c1
- Derived from:
- ccsbBroadEn_10030
- DNA Barcode:
- TAGCCTAACGACCATTAGCCTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR10A2 (341276)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491481
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 341276 | OR10A2 | olfactory receptor family 1... | NM_001004460.1 | 99.8% | 99.6% | 400G>A |
| 2 | human | 144124 | OR10A5 | olfactory receptor family 1... | NM_178168.1 | 91% | 91.1% | (many diffs) |
| 3 | mouse | 259035 | Olfr714 | olfactory receptor 714 | NM_147033.2 | 83.9% | 85.1% | (many diffs) |
| 4 | mouse | 259036 | Olfr713 | olfactory receptor 713 | NM_147034.1 | 83.2% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 978
- ORF length:
- 909
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcttctct tccctgccta ctgaaataca gtcattactc tttctgacat 121 ttctaaccat ctacctggtc accctgatgg gaaactgcct catcattctg gttaccctag 181 ctgaccccat gctacacagc cccatgtact tcttcctcag aaacttatct ttcctggaga 241 ttggcttcaa cctagtcatt gtgcccaaaa tgctggggac cctgcttgcc caggacacaa 301 ccatctcctt ccttggctgt gccactcaga tgtatttctt cttcttcttt ggagtggctg 361 aatgcttcct cctggctacc atggcatatg accgctatgt ggccatctgc agtcccttgc 421 actacccagt catcatgaac caaaggactc gtgccaaact ggctgctacc tcctggttcc 481 caggctttcc tgtagctact gtgcagacca catggctctt cagttttcca ttctgtggca 541 ccaacaaggt gaaccacttc ttctgtgaca gcccacctgt gctgaggctg gtctgtgcag 601 acacagcact ctttgagatc tacgccatcg tcggaaccat tctggtggtc atgatcccct 661 gcttgctgat cttgtgttcc tatactcaca ttgctgctgc catcctcaag atcccatcag 721 ctaaagggaa gaataaagcc ttttctacat gttcctcaca cctCCTTGTT GTCTCTCTTT 781 TCTATATATC ATTAAGCCTC ACCTACTTCC GGCCTAAATC AAATAATTCA CCTGAGGGCA 841 AGAAGCTGCT ATCATTGTCC TACACTGTTA TGACTCCCAT GTTGAACCCC ATTATCTACA 901 GCCTGAGAAA TAACGAGGTG AAGAATGCCC TCAGCAGGAC GGTCTCTAAG GCCCTAGCCC 961 TCAGAAACTG TATCCCATTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1021 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1081 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATAGCCTA ACGACCATTA 1141 GCCTCAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt