Construct: ORF TRCN0000491495
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021727.2_s317c1
- DNA Barcode:
- GGCGCTTACCCTTACAACAATAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DDR2 (4921)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491495
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4921 | DDR2 | discoidin domain receptor t... | NM_001014796.3 | 49.1% | 49.1% | 1_1305del |
| 2 | human | 4921 | DDR2 | discoidin domain receptor t... | NM_001354982.2 | 49.1% | 49.1% | 1_1305del |
| 3 | human | 4921 | DDR2 | discoidin domain receptor t... | NM_001354983.2 | 49.1% | 49.1% | 1_1305del |
| 4 | human | 4921 | DDR2 | discoidin domain receptor t... | NM_006182.4 | 49.1% | 49.1% | 1_1305del |
| 5 | human | 4921 | DDR2 | discoidin domain receptor t... | XM_011509587.2 | 49.1% | 49.1% | 1_1305del |
| 6 | mouse | 18214 | Ddr2 | discoidin domain receptor f... | NM_022563.2 | 44% | 46.9% | (many diffs) |
| 7 | mouse | 18214 | Ddr2 | discoidin domain receptor f... | XM_006496696.3 | 44% | 46.9% | (many diffs) |
| 8 | mouse | 18214 | Ddr2 | discoidin domain receptor f... | XM_006496697.3 | 44% | 46.9% | (many diffs) |
| 9 | mouse | 18214 | Ddr2 | discoidin domain receptor f... | XM_006496698.3 | 44% | 46.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1335
- ORF length:
- 1260
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctctcg gaggatgctg gatgatgaaa tgacagtcag cctttccctg ccaagtgatt 121 ctagcatgtt caacaataac cgctcctcat cacctagtga acaagggtcc aactcgactt 181 acgatcgcat ctttcccctt cgccctgact accaggagcc atccaggctg atacgaaaac 241 tcccagaatt tgctccaggg gaggaggagt caggctgcag cggtgttgtg aagccagtcc 301 agcccagtgg ccctgagggg gtgccccact atgcagaggc tgacatagtg aacctccaag 361 gagtgacagg aggcaacaca tactcagtgc ctgccgtcac catggacctg ctctcaggaa 421 aagatgtggc tgtggaggag ttccccagga aactcctaac tttcaaagag aagctgggag 481 aaggacagtt tggggaggtt catctctgtg aagtggaggg aatggaaaaa ttcaaagaca 541 aagattttgc cctagatgtc agtgccaacc agcctgtcct ggtggctgtg aaaatgctcc 601 gagcagatgc caacaagaat gccaggaatg attttcttaa ggagataaag atcatgtctc 661 ggctcaagga cccaaacatc atccatctat tagctgtgtg tatcactgat gaccctctct 721 gtatgatcac tgaatacatg gagaatggag atctcaatca gtttctttcc cgccacgagc 781 cccctaattc ttcctccagc gatgtacgca ctgtcagtta caccaatctg aagtttatgg 841 ctacccaaat tgcctctggc atgaagtacc tttcctctct taattttgtt caccgagatc 901 tggccacacg aaactgttta gtgggtaaga actacacaat caagatagct gactttggaa 961 tgagcaggaa cctgtacagt ggtgactatt accggatcca gggccgggca gtgctcccta 1021 tccgctGGAT GTCTTGGGAG AGTATCTTGC TGGGCAAGTT CACTACAGCA AGTGATGTGT 1081 GGGCCTTTGG GGTTACTTTG TGGGAGACTT TCACCTTTTG TCAAGAACAG CCCTATTCCC 1141 AGCTGTCAGA TGAACAGGTT ATTGAGAATA CTGGAGAGTT CTTCCGAGAC CAAGGGAGGC 1201 AGACTTACCT CCCTCAACCA GCCATTTGTC CTGACTCTGT GTATAAGCTG ATGCTCAGCT 1261 GCTGGAGAAG AGATACGAAG AACCGTCCCT CATTCCAAGA AATCCACCTT CTGCTCCTTC 1321 AACAAGGCGA CGAGTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1381 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1441 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGCGCTT ACCCTTACAA 1501 CAATAGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt