Construct: ORF TRCN0000491499
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009155.1_s317c1
- Derived from:
- ccsbBroadEn_14236
- DNA Barcode:
- GCGGTGGAGGTTTGCACTACCTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- WDR19 (57728)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491499
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57728 | WDR19 | WD repeat domain 19 | XR_001741312.1 | 52.2% | (many diffs) | |
| 2 | human | 57728 | WDR19 | WD repeat domain 19 | NM_025132.4 | 32.4% | 31.3% | (many diffs) |
| 3 | human | 57728 | WDR19 | WD repeat domain 19 | XM_011513724.1 | 32.3% | 31.2% | (many diffs) |
| 4 | human | 57728 | WDR19 | WD repeat domain 19 | XM_011513725.2 | 30.7% | 29.6% | (many diffs) |
| 5 | human | 57728 | WDR19 | WD repeat domain 19 | XR_001741307.1 | 28.2% | (many diffs) | |
| 6 | human | 57728 | WDR19 | WD repeat domain 19 | XR_001741306.1 | 28.1% | (many diffs) | |
| 7 | human | 57728 | WDR19 | WD repeat domain 19 | XR_001741309.1 | 21.5% | (many diffs) | |
| 8 | human | 57728 | WDR19 | WD repeat domain 19 | XR_925155.1 | 21.5% | (many diffs) | |
| 9 | human | 57728 | WDR19 | WD repeat domain 19 | XR_001741310.1 | 20.8% | (many diffs) | |
| 10 | human | 57728 | WDR19 | WD repeat domain 19 | XR_001741308.1 | 20.8% | (many diffs) | |
| 11 | human | 57728 | WDR19 | WD repeat domain 19 | NM_001317924.2 | 20.5% | 19.4% | (many diffs) |
| 12 | human | 57728 | WDR19 | WD repeat domain 19 | XM_017008501.1 | 20.5% | 19.4% | (many diffs) |
| 13 | human | 57728 | WDR19 | WD repeat domain 19 | XM_011513726.3 | 20.4% | 19.3% | (many diffs) |
| 14 | human | 57728 | WDR19 | WD repeat domain 19 | XM_011513727.1 | 20.4% | 19.3% | (many diffs) |
| 15 | human | 57728 | WDR19 | WD repeat domain 19 | XR_001741311.2 | 19.7% | (many diffs) | |
| 16 | mouse | 213081 | Wdr19 | WD repeat domain 19 | NM_153391.2 | 28.2% | 26.9% | (many diffs) |
| 17 | mouse | 213081 | Wdr19 | WD repeat domain 19 | XM_011240713.1 | 28.2% | 26.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1365
- ORF length:
- 1299
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcgtattttc tcactgctag aaaagacttg gcttggcgca ccaatacagt 121 ttgcctggca aaaaacatca ggaaactacc ttgcagtaac aggagctgat tatattgtga 181 aaatctttga tcgccatggt caaaaaagaa gtgaaattaa cttacctggt aactgtgttg 241 ccatggattg ggataaagat ggagatgtcc tagcagtgat tgctgagaaa tctagctgca 301 tttatctttg ggatgccaac acaaataaga ccagccagtt agacaatggc atgagggatc 361 aaatgtcttt ccttctttgg tcaaaagttg gaagtttcct ggctgttgga actgttaaag 421 gaaatttgct tatttataat catcagacat ctcgaaagat tcctgtcctt ggaaaacata 481 ctaagagaat cacttgtgga tgttggaatg cagaaaatct gcttgcttta ggtggtgaag 541 ataaaatgat tacagttagt aatcaggaag gtgacacgat aagacagaca caagtgagat 601 cagagcctag caacatgcag tttttcttga tgaagatgga tgaccgaacc tctgctgctg 661 aaagcatgat aagtgtggtg cttggcaaga aaactttgtt ttttttaaat ctgaatgaac 721 cagataaccc agctgatctt gaatttcagc aggactttgg caacattgtc tgctataatt 781 ggtatggtga tggccgcatc atgattggtt tttcatgtgg acattttgtg gtcatttcta 841 ctcatactgg agagcttggt caagagatat ttcaggctcg taaccataaa gataatctaa 901 ccagcattgc agtatcacag actcttaaca aagttgctac atgtggagat aactgtatta 961 aaatccaaga cttggttgac ttaaaagaca tgtatgttat actcaacctG GATGAGGAAA 1021 ATAAAGGATT GGGTACCTTG TCCTGGACTG ATGATGGCCA GTTGCTAGCA CTCTCTACCC 1081 AAAGGGGCTC ACTTCATGTT TTCCTGACCA AGCTTCCCAT ACTTGGGGAT GCCTGCAGCA 1141 CAAGGATTGC CTATCTCACC TCCCTCCTTG AAGTCACCGT AGCCAACCCT GTTGAAGGAG 1201 AGCTACCAAT CACAGTTTCT GTTGATGTGG AACCCAACTT TGTGGCAGTA GGTCTTTATC 1261 ATCTGGCTGT AGGAATGAAT AATCGAGCTT GGTTTTATGT CCTTGGAGAA AATGCTGTGA 1321 AAAAAATTGA AAGATATGGA GTATCTGGGA ACAGTAGCCA GTATTAGCCT TCATGCTGCC 1381 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1441 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1501 ATATATCTTG TGGAAAGGAC GAGCGGTGGA GGTTTGCACT ACCTTTACGC GTTAAGTCga 1561 caatcaacct ctggattaca aaatttgtga aagatt