Construct: ORF TRCN0000491502
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006649.1_s317c1
- Derived from:
- ccsbBroadEn_12927
- DNA Barcode:
- TCGTACGAGGCGCGCACCTTGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM116 (89894)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491502
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538958.1 | 94.1% | 94.1% | 1_39del |
| 2 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449267.1 | 94.1% | 94.1% | 1_39del |
| 3 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449268.1 | 94.1% | 94.1% | 1_39del |
| 4 | human | 89894 | TMEM116 | transmembrane protein 116 | NM_001294314.1 | 85.3% | 85.3% | 1_108del |
| 5 | human | 89894 | TMEM116 | transmembrane protein 116 | NM_138341.2 | 85.3% | 85.3% | 1_108del |
| 6 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538957.1 | 85.3% | 85.3% | 1_108del |
| 7 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_017020190.2 | 85.3% | 85.3% | 1_108del |
| 8 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_017020192.2 | 85.3% | 85.3% | 1_108del |
| 9 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449265.1 | 85.3% | 85.3% | 1_108del |
| 10 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538953.1 | 76.2% | 76.2% | 1_195del |
| 11 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_017020188.2 | 76.2% | 76.2% | 1_195del |
| 12 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449263.1 | 76.2% | 76.2% | 1_195del |
| 13 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449264.1 | 76.2% | 76.2% | 1_195del |
| 14 | human | 89894 | TMEM116 | transmembrane protein 116 | NM_001193453.1 | 69.2% | 69.2% | 1_279del |
| 15 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538947.2 | 69.2% | 69.2% | 1_279del |
| 16 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538951.2 | 65.3% | 65.3% | 1_333del |
| 17 | human | 89894 | TMEM116 | transmembrane protein 116 | NM_001193531.2 | 62% | 62% | 1_384del |
| 18 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538949.2 | 62% | 62% | 1_384del |
| 19 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449260.1 | 62% | 62% | 1_384del |
| 20 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_006719678.3 | 57.4% | 57.4% | 1_465del |
| 21 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449259.1 | 56.3% | 56.3% | 1_486del |
| 22 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449258.1 | 53.8% | 53.8% | 1_537del |
| 23 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449257.1 | 53% | 53% | 1_555del |
| 24 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449266.1 | 48.6% | 39.1% | 1_279del;624_625ins74;720_721ins112 |
| 25 | human | 89894 | TMEM116 | transmembrane protein 116 | XR_001748914.2 | 47.9% | 1_484del;831_902del;1184_1307del | |
| 26 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538948.3 | 47.5% | 43.3% | (many diffs) |
| 27 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538950.2 | 43.6% | 35.1% | 1_384del;729_730ins74;825_826ins112 |
| 28 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449262.1 | 43.6% | 35.1% | 1_384del;729_730ins74;825_826ins112 |
| 29 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449261.1 | 39.6% | 32% | 1_486del;831_832ins74;927_928ins112 |
| 30 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_017020187.2 | 37% | 33.1% | (many diffs) |
| 31 | human | 89894 | TMEM116 | transmembrane protein 116 | NR_122119.1 | 31.6% | 1_571del;636_637ins139;1060_1404del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 693
- ORF length:
- 627
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac acctgtattc tgtctgggaa atactagtga atgtttccaa aacttcagtc 121 agagccacaa gtgtatcttg atgcactcac caccatcagc catggctgaa cttccacctt 181 ctgccaacac atctgtctgt agcacacttt atttttatgg tatcgccatt ttcctgggca 241 gctttgtact cagcctcctt accattatgg tcttacttat ccgagcccag acattgtata 301 agaagtttgt gaagtcaact ggctttctgg ggagtgaaca gtgggcagtg attcacattg 361 tGGACCAACG GGTGCGCTTC TACCCAGTGG CCTTCTTTTG CTGCTGGGGC CCAGCTGTCA 421 TTCTAATGAT CATAAAGCTG ACTAAGCCAC AGGACACCAA GCTTCACATG GCCCTTTATG 481 TTCTCCAGGC TCTAACGGCA ACATCTCAGG GTCTACTCAA CTGTGGAGTA TATGGCTGGA 541 CGCAGCACAA ATTCCACCAA CTAAAGCAGG AGGCTCGGCG TGATGCAGAT ACCCAGACAC 601 CATTATTATG CTCACAGAAG AGATTCTATA GCAGGGGCTT AAATTCACTG GAATCCACCC 661 TGACTTTTCC TGCCAGTACT TCTACCATTT TTTGCCCAAC TTTCTTGTAC AAAGTGGTTG 721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 841 GTACGAGGCG CGCACCTTGT CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 901 ttgtgaaaga tt